NEFL is overexpressed and it modulates invasion and migration in neuroendocrine-like PC3-ML2 prostate cancer cells

Prostate cancer clinical outcomes are varied, from non-aggressive asymptomatic to lethal aggressive neuroendocrine forms which represent a critical challenge in the management of the disease. The neurofilament light ( NEFL ) is proposed to be a tumor suppressor gene. Studies have shown that expressi...

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Detalles Bibliográficos
Autores principales: Burch, Tanya C, Mackay, Stephen, Hitefield, Naomi L, Roberts, Autumn B, Oduor, Ian O, Nyalwidhe, Julius O
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636496/
https://www.ncbi.nlm.nih.gov/pubmed/36345474
http://dx.doi.org/10.17912/micropub.biology.000658
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author Burch, Tanya C
Mackay, Stephen
Hitefield, Naomi L
Roberts, Autumn B
Oduor, Ian O
Nyalwidhe, Julius O
author_facet Burch, Tanya C
Mackay, Stephen
Hitefield, Naomi L
Roberts, Autumn B
Oduor, Ian O
Nyalwidhe, Julius O
author_sort Burch, Tanya C
collection PubMed
description Prostate cancer clinical outcomes are varied, from non-aggressive asymptomatic to lethal aggressive neuroendocrine forms which represent a critical challenge in the management of the disease. The neurofilament light ( NEFL ) is proposed to be a tumor suppressor gene. Studies have shown that expression of the gene is decreased in various cancers. We have used quantitative RT-PCR, immunoblotting, methylation specific PCR, siRNA knockdown followed by migration/invasion assays to determine associations between NEFL expression and disease phenotype in a panel of prostate cells. We demonstrate that NEFL is overexpressed and it modulates invasion and migration in PC3-ML2 prostate cancers cells which have an aggressive neuroendocrine-like phenotype.
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spelling pubmed-96364962022-11-06 NEFL is overexpressed and it modulates invasion and migration in neuroendocrine-like PC3-ML2 prostate cancer cells Burch, Tanya C Mackay, Stephen Hitefield, Naomi L Roberts, Autumn B Oduor, Ian O Nyalwidhe, Julius O MicroPubl Biol New Finding Prostate cancer clinical outcomes are varied, from non-aggressive asymptomatic to lethal aggressive neuroendocrine forms which represent a critical challenge in the management of the disease. The neurofilament light ( NEFL ) is proposed to be a tumor suppressor gene. Studies have shown that expression of the gene is decreased in various cancers. We have used quantitative RT-PCR, immunoblotting, methylation specific PCR, siRNA knockdown followed by migration/invasion assays to determine associations between NEFL expression and disease phenotype in a panel of prostate cells. We demonstrate that NEFL is overexpressed and it modulates invasion and migration in PC3-ML2 prostate cancers cells which have an aggressive neuroendocrine-like phenotype. Caltech Library 2022-10-22 /pmc/articles/PMC9636496/ /pubmed/36345474 http://dx.doi.org/10.17912/micropub.biology.000658 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Burch, Tanya C
Mackay, Stephen
Hitefield, Naomi L
Roberts, Autumn B
Oduor, Ian O
Nyalwidhe, Julius O
NEFL is overexpressed and it modulates invasion and migration in neuroendocrine-like PC3-ML2 prostate cancer cells
title NEFL is overexpressed and it modulates invasion and migration in neuroendocrine-like PC3-ML2 prostate cancer cells
title_full NEFL is overexpressed and it modulates invasion and migration in neuroendocrine-like PC3-ML2 prostate cancer cells
title_fullStr NEFL is overexpressed and it modulates invasion and migration in neuroendocrine-like PC3-ML2 prostate cancer cells
title_full_unstemmed NEFL is overexpressed and it modulates invasion and migration in neuroendocrine-like PC3-ML2 prostate cancer cells
title_short NEFL is overexpressed and it modulates invasion and migration in neuroendocrine-like PC3-ML2 prostate cancer cells
title_sort nefl is overexpressed and it modulates invasion and migration in neuroendocrine-like pc3-ml2 prostate cancer cells
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636496/
https://www.ncbi.nlm.nih.gov/pubmed/36345474
http://dx.doi.org/10.17912/micropub.biology.000658
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