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Transcriptional and biochemical analyses of Planomicrobium strain AX6 from Qinghai-Tibetan Plateau, China, reveal hydrogen peroxide scavenging potential

BACKGROUND: The bacterial mechanisms responsible for hydrogen peroxide (H(2)O(2)) scavenging have been well-reported, yet little is known about how bacteria isolated from cold-environments respond to H(2)O(2) stress. Therefore, we investigated the transcriptional profiling of the Planomicrobium stra...

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Autores principales: Bahadur, Ali, Li, Ting, Sajjad, Wasim, Nasir, Fahad, Zia, Muhammad Amir, Wu, Minghui, Zhang, Gaosen, Liu, Guangxiu, Chen, Tuo, Zhang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636757/
https://www.ncbi.nlm.nih.gov/pubmed/36335290
http://dx.doi.org/10.1186/s12866-022-02677-w
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author Bahadur, Ali
Li, Ting
Sajjad, Wasim
Nasir, Fahad
Zia, Muhammad Amir
Wu, Minghui
Zhang, Gaosen
Liu, Guangxiu
Chen, Tuo
Zhang, Wei
author_facet Bahadur, Ali
Li, Ting
Sajjad, Wasim
Nasir, Fahad
Zia, Muhammad Amir
Wu, Minghui
Zhang, Gaosen
Liu, Guangxiu
Chen, Tuo
Zhang, Wei
author_sort Bahadur, Ali
collection PubMed
description BACKGROUND: The bacterial mechanisms responsible for hydrogen peroxide (H(2)O(2)) scavenging have been well-reported, yet little is known about how bacteria isolated from cold-environments respond to H(2)O(2) stress. Therefore, we investigated the transcriptional profiling of the Planomicrobium strain AX6 strain isolated from the cold-desert ecosystem in the Qaidam Basin, Qinghai-Tibet Plateau, China, in response to H(2)O(2) stress aiming to uncover the molecular mechanisms associated with H(2)O(2) scavenging potential. METHODS: We investigated the H(2)O(2)-scavenging potential of the bacterial Planomicrobium strain AX6 isolated from the cold-desert ecosystem in the Qaidam Basin, Qinghai-Tibet Plateau, China. Furthermore, we used high-throughput RNA-sequencing to unravel the molecular aspects associated with the H(2)O(2) scavenging potential of the Planomicrobium strain AX6 isolate. RESULTS: In total, 3,427 differentially expressed genes (DEGs) were identified in Planomicrobium strain AX6 isolate in response to 4 h of H(2)O(2) (1.5 mM) exposure. Besides, Kyoto Encyclopedia of Genes and Genomes pathway and Gene Ontology analyses revealed the down- and/or up-regulated pathways following H(2)O(2) treatment. Our study not only identified the H(2)O(2) scavenging capability of the strain nevertheless also a range of mechanisms to cope with the toxic effect of H(2)O(2) through genes involved in oxidative stress response. Compared to control, several genes coding for antioxidant proteins, including glutathione peroxidase (GSH-Px), Coproporphyrinogen III oxidase, and superoxide dismutase (SOD), were relatively up-regulated in Planomicrobium strain AX6, when exposed to H(2)O(2). CONCLUSIONS: Overall, the results suggest that the up-regulated genes responsible for antioxidant defense pathways serve as essential regulatory mechanisms for removing H(2)O(2) in Planomicrobium strain AX6. The DEGs identified here could provide a competitive advantage for the existence of Planomicrobium strain AX6 in H(2)O(2)-polluted environments. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-022-02677-w.
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spelling pubmed-96367572022-11-06 Transcriptional and biochemical analyses of Planomicrobium strain AX6 from Qinghai-Tibetan Plateau, China, reveal hydrogen peroxide scavenging potential Bahadur, Ali Li, Ting Sajjad, Wasim Nasir, Fahad Zia, Muhammad Amir Wu, Minghui Zhang, Gaosen Liu, Guangxiu Chen, Tuo Zhang, Wei BMC Microbiol Research BACKGROUND: The bacterial mechanisms responsible for hydrogen peroxide (H(2)O(2)) scavenging have been well-reported, yet little is known about how bacteria isolated from cold-environments respond to H(2)O(2) stress. Therefore, we investigated the transcriptional profiling of the Planomicrobium strain AX6 strain isolated from the cold-desert ecosystem in the Qaidam Basin, Qinghai-Tibet Plateau, China, in response to H(2)O(2) stress aiming to uncover the molecular mechanisms associated with H(2)O(2) scavenging potential. METHODS: We investigated the H(2)O(2)-scavenging potential of the bacterial Planomicrobium strain AX6 isolated from the cold-desert ecosystem in the Qaidam Basin, Qinghai-Tibet Plateau, China. Furthermore, we used high-throughput RNA-sequencing to unravel the molecular aspects associated with the H(2)O(2) scavenging potential of the Planomicrobium strain AX6 isolate. RESULTS: In total, 3,427 differentially expressed genes (DEGs) were identified in Planomicrobium strain AX6 isolate in response to 4 h of H(2)O(2) (1.5 mM) exposure. Besides, Kyoto Encyclopedia of Genes and Genomes pathway and Gene Ontology analyses revealed the down- and/or up-regulated pathways following H(2)O(2) treatment. Our study not only identified the H(2)O(2) scavenging capability of the strain nevertheless also a range of mechanisms to cope with the toxic effect of H(2)O(2) through genes involved in oxidative stress response. Compared to control, several genes coding for antioxidant proteins, including glutathione peroxidase (GSH-Px), Coproporphyrinogen III oxidase, and superoxide dismutase (SOD), were relatively up-regulated in Planomicrobium strain AX6, when exposed to H(2)O(2). CONCLUSIONS: Overall, the results suggest that the up-regulated genes responsible for antioxidant defense pathways serve as essential regulatory mechanisms for removing H(2)O(2) in Planomicrobium strain AX6. The DEGs identified here could provide a competitive advantage for the existence of Planomicrobium strain AX6 in H(2)O(2)-polluted environments. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-022-02677-w. BioMed Central 2022-11-05 /pmc/articles/PMC9636757/ /pubmed/36335290 http://dx.doi.org/10.1186/s12866-022-02677-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Bahadur, Ali
Li, Ting
Sajjad, Wasim
Nasir, Fahad
Zia, Muhammad Amir
Wu, Minghui
Zhang, Gaosen
Liu, Guangxiu
Chen, Tuo
Zhang, Wei
Transcriptional and biochemical analyses of Planomicrobium strain AX6 from Qinghai-Tibetan Plateau, China, reveal hydrogen peroxide scavenging potential
title Transcriptional and biochemical analyses of Planomicrobium strain AX6 from Qinghai-Tibetan Plateau, China, reveal hydrogen peroxide scavenging potential
title_full Transcriptional and biochemical analyses of Planomicrobium strain AX6 from Qinghai-Tibetan Plateau, China, reveal hydrogen peroxide scavenging potential
title_fullStr Transcriptional and biochemical analyses of Planomicrobium strain AX6 from Qinghai-Tibetan Plateau, China, reveal hydrogen peroxide scavenging potential
title_full_unstemmed Transcriptional and biochemical analyses of Planomicrobium strain AX6 from Qinghai-Tibetan Plateau, China, reveal hydrogen peroxide scavenging potential
title_short Transcriptional and biochemical analyses of Planomicrobium strain AX6 from Qinghai-Tibetan Plateau, China, reveal hydrogen peroxide scavenging potential
title_sort transcriptional and biochemical analyses of planomicrobium strain ax6 from qinghai-tibetan plateau, china, reveal hydrogen peroxide scavenging potential
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636757/
https://www.ncbi.nlm.nih.gov/pubmed/36335290
http://dx.doi.org/10.1186/s12866-022-02677-w
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