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Metagenomic and proteomic analysis of bacterial retting community and proteome profile in the degumming process of kenaf bast
BACKGROUND: Data on the microbial community and functional proteins associated with degumming in kenaf remains scant. Here, we analyzed the microbial communities associated with kenaf (Hibiscus cannabinus) bast fibers during retting to identify potential candidate degumming bacteria. Retting liquids...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636830/ https://www.ncbi.nlm.nih.gov/pubmed/36333799 http://dx.doi.org/10.1186/s12870-022-03890-5 |
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author | Xu, Huan Zhang, Lixia Feng, Xiangyuan Yang, Qi Zheng, Ke Duan, Shengwen Cheng, Lifeng |
author_facet | Xu, Huan Zhang, Lixia Feng, Xiangyuan Yang, Qi Zheng, Ke Duan, Shengwen Cheng, Lifeng |
author_sort | Xu, Huan |
collection | PubMed |
description | BACKGROUND: Data on the microbial community and functional proteins associated with degumming in kenaf remains scant. Here, we analyzed the microbial communities associated with kenaf (Hibiscus cannabinus) bast fibers during retting to identify potential candidate degumming bacteria. Retting liquids were collected and analyzed at 0 days, 10 days, and 34 days and then evaluated the yield and quality of kenaf fiber at the different retting times. Besides, the microbial communities were characterized using metagenomic and proteomic analysis by LC–MS/MS technology. RESULTS: The data showed that increase in the retting time significantly improves the softness, dispersion, and fiber whiteness of the kenaf fiber. The relative abundance of Acinetobacter increased from 2.88% at the baseline to 6.64% at the 34th retting. On the other hand, some members of Clostridium were reduced from 3% at the baseline to 2% at the 34th retting. Analysis of carbohydrate active enzymes showed constant changes in the utilization of carbohydrates. Besides, benzoquinone reductase, cellobiose dehydrogenase, glucose 1-oxidase, aryl alcohol oxidase and alcohol oxidase were the top five most abundant enzymes in the retting liquids. This present results demonstrated that the expressions of B7GYR8, Q6RYW5 and Q6FFK2 proteins were suppressed in Acinetobacter with the retting time. On the contrary, P05149 was upregulated with the retting time. In Clostridium, P37698, P52040 and P54937 proteins were upregulated with the retting time. CONCLUSION: In addition, bacteria Acinetobacter and Clostridium might be playing important roles in the kenaf degumming process. Similarly, up-regulation of P37698, P52040 and P54937 proteins is an important manifestation and mediates important roles in the degumming process. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03890-5. |
format | Online Article Text |
id | pubmed-9636830 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-96368302022-11-06 Metagenomic and proteomic analysis of bacterial retting community and proteome profile in the degumming process of kenaf bast Xu, Huan Zhang, Lixia Feng, Xiangyuan Yang, Qi Zheng, Ke Duan, Shengwen Cheng, Lifeng BMC Plant Biol Research BACKGROUND: Data on the microbial community and functional proteins associated with degumming in kenaf remains scant. Here, we analyzed the microbial communities associated with kenaf (Hibiscus cannabinus) bast fibers during retting to identify potential candidate degumming bacteria. Retting liquids were collected and analyzed at 0 days, 10 days, and 34 days and then evaluated the yield and quality of kenaf fiber at the different retting times. Besides, the microbial communities were characterized using metagenomic and proteomic analysis by LC–MS/MS technology. RESULTS: The data showed that increase in the retting time significantly improves the softness, dispersion, and fiber whiteness of the kenaf fiber. The relative abundance of Acinetobacter increased from 2.88% at the baseline to 6.64% at the 34th retting. On the other hand, some members of Clostridium were reduced from 3% at the baseline to 2% at the 34th retting. Analysis of carbohydrate active enzymes showed constant changes in the utilization of carbohydrates. Besides, benzoquinone reductase, cellobiose dehydrogenase, glucose 1-oxidase, aryl alcohol oxidase and alcohol oxidase were the top five most abundant enzymes in the retting liquids. This present results demonstrated that the expressions of B7GYR8, Q6RYW5 and Q6FFK2 proteins were suppressed in Acinetobacter with the retting time. On the contrary, P05149 was upregulated with the retting time. In Clostridium, P37698, P52040 and P54937 proteins were upregulated with the retting time. CONCLUSION: In addition, bacteria Acinetobacter and Clostridium might be playing important roles in the kenaf degumming process. Similarly, up-regulation of P37698, P52040 and P54937 proteins is an important manifestation and mediates important roles in the degumming process. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03890-5. BioMed Central 2022-11-05 /pmc/articles/PMC9636830/ /pubmed/36333799 http://dx.doi.org/10.1186/s12870-022-03890-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Xu, Huan Zhang, Lixia Feng, Xiangyuan Yang, Qi Zheng, Ke Duan, Shengwen Cheng, Lifeng Metagenomic and proteomic analysis of bacterial retting community and proteome profile in the degumming process of kenaf bast |
title | Metagenomic and proteomic analysis of bacterial retting community and proteome profile in the degumming process of kenaf bast |
title_full | Metagenomic and proteomic analysis of bacterial retting community and proteome profile in the degumming process of kenaf bast |
title_fullStr | Metagenomic and proteomic analysis of bacterial retting community and proteome profile in the degumming process of kenaf bast |
title_full_unstemmed | Metagenomic and proteomic analysis of bacterial retting community and proteome profile in the degumming process of kenaf bast |
title_short | Metagenomic and proteomic analysis of bacterial retting community and proteome profile in the degumming process of kenaf bast |
title_sort | metagenomic and proteomic analysis of bacterial retting community and proteome profile in the degumming process of kenaf bast |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636830/ https://www.ncbi.nlm.nih.gov/pubmed/36333799 http://dx.doi.org/10.1186/s12870-022-03890-5 |
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