Cargando…
Transcription Factor Hb9 Is Expressed in Glial Cell Lineages in the Developing Mouse Spinal Cord
Hb9 (Mnx1) is a transcription factor described as a spinal cord motor neuron (MN)-specific marker and critical factor for the postmitotic specification of these cells. To date, expression of Hb9 in other cell types has not been reported. We performed a fate-mapping approach to examine distributions...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society for Neuroscience
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636997/ https://www.ncbi.nlm.nih.gov/pubmed/36265906 http://dx.doi.org/10.1523/ENEURO.0214-22.2022 |
_version_ | 1784825079318708224 |
---|---|
author | Letchuman, Sunjay Tucker, Ashley Miranda, Diego Adkins, Robert L. Aceves, Miriam Dietz, Valerie Jagrit, Vipin Leonards, Amy Lee, Young il Dulin, Jennifer N. |
author_facet | Letchuman, Sunjay Tucker, Ashley Miranda, Diego Adkins, Robert L. Aceves, Miriam Dietz, Valerie Jagrit, Vipin Leonards, Amy Lee, Young il Dulin, Jennifer N. |
author_sort | Letchuman, Sunjay |
collection | PubMed |
description | Hb9 (Mnx1) is a transcription factor described as a spinal cord motor neuron (MN)-specific marker and critical factor for the postmitotic specification of these cells. To date, expression of Hb9 in other cell types has not been reported. We performed a fate-mapping approach to examine distributions of Hb9-expressing cells and their progeny (“Hb9-lineage cells”) within the embryonic and adult spinal cord of Hb9(cre);Ai14 mice. We found that Hb9-lineage cells are distributed in a gradient of increasing abundance throughout the rostrocaudal spinal cord axis during embryonic and postnatal stages. Furthermore, although the majority of Hb9-lineage cells at cervical spinal cord levels are MNs, at more caudal levels, Hb9-lineage cells include small-diameter dorsal horn neurons, astrocytes, and oligodendrocytes. In the peripheral nervous system, we observed a similar phenomenon with more abundant Hb9-lineage Schwann cells in muscles of the lower body versus upper body muscles. We cultured spinal cord progenitors in vitro and found that gliogenesis was increased by treatment with the caudalizing factor FGF-8B, while glial tdTomato expression was increased by treatment with both FGF-8B and GDF-11. Together, these observations suggest that early and transient expression of Hb9 in spinal cord neural progenitors may be induced by caudalizing factors such as FGF and GDF signaling. Furthermore, our work raises the possibility that early Hb9 expression may influence the development of spinal cord macroglia and Schwann cells, especially at caudal regions. Together, these findings highlight the importance of using caution when designing experiments using Hb9(cre) mice to perform spinal cord MN-specific manipulations. |
format | Online Article Text |
id | pubmed-9636997 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Society for Neuroscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-96369972022-11-07 Transcription Factor Hb9 Is Expressed in Glial Cell Lineages in the Developing Mouse Spinal Cord Letchuman, Sunjay Tucker, Ashley Miranda, Diego Adkins, Robert L. Aceves, Miriam Dietz, Valerie Jagrit, Vipin Leonards, Amy Lee, Young il Dulin, Jennifer N. eNeuro Research Article: New Research Hb9 (Mnx1) is a transcription factor described as a spinal cord motor neuron (MN)-specific marker and critical factor for the postmitotic specification of these cells. To date, expression of Hb9 in other cell types has not been reported. We performed a fate-mapping approach to examine distributions of Hb9-expressing cells and their progeny (“Hb9-lineage cells”) within the embryonic and adult spinal cord of Hb9(cre);Ai14 mice. We found that Hb9-lineage cells are distributed in a gradient of increasing abundance throughout the rostrocaudal spinal cord axis during embryonic and postnatal stages. Furthermore, although the majority of Hb9-lineage cells at cervical spinal cord levels are MNs, at more caudal levels, Hb9-lineage cells include small-diameter dorsal horn neurons, astrocytes, and oligodendrocytes. In the peripheral nervous system, we observed a similar phenomenon with more abundant Hb9-lineage Schwann cells in muscles of the lower body versus upper body muscles. We cultured spinal cord progenitors in vitro and found that gliogenesis was increased by treatment with the caudalizing factor FGF-8B, while glial tdTomato expression was increased by treatment with both FGF-8B and GDF-11. Together, these observations suggest that early and transient expression of Hb9 in spinal cord neural progenitors may be induced by caudalizing factors such as FGF and GDF signaling. Furthermore, our work raises the possibility that early Hb9 expression may influence the development of spinal cord macroglia and Schwann cells, especially at caudal regions. Together, these findings highlight the importance of using caution when designing experiments using Hb9(cre) mice to perform spinal cord MN-specific manipulations. Society for Neuroscience 2022-11-02 /pmc/articles/PMC9636997/ /pubmed/36265906 http://dx.doi.org/10.1523/ENEURO.0214-22.2022 Text en Copyright © 2022 Letchuman et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article: New Research Letchuman, Sunjay Tucker, Ashley Miranda, Diego Adkins, Robert L. Aceves, Miriam Dietz, Valerie Jagrit, Vipin Leonards, Amy Lee, Young il Dulin, Jennifer N. Transcription Factor Hb9 Is Expressed in Glial Cell Lineages in the Developing Mouse Spinal Cord |
title | Transcription Factor Hb9 Is Expressed in Glial Cell Lineages in the Developing Mouse Spinal Cord |
title_full | Transcription Factor Hb9 Is Expressed in Glial Cell Lineages in the Developing Mouse Spinal Cord |
title_fullStr | Transcription Factor Hb9 Is Expressed in Glial Cell Lineages in the Developing Mouse Spinal Cord |
title_full_unstemmed | Transcription Factor Hb9 Is Expressed in Glial Cell Lineages in the Developing Mouse Spinal Cord |
title_short | Transcription Factor Hb9 Is Expressed in Glial Cell Lineages in the Developing Mouse Spinal Cord |
title_sort | transcription factor hb9 is expressed in glial cell lineages in the developing mouse spinal cord |
topic | Research Article: New Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636997/ https://www.ncbi.nlm.nih.gov/pubmed/36265906 http://dx.doi.org/10.1523/ENEURO.0214-22.2022 |
work_keys_str_mv | AT letchumansunjay transcriptionfactorhb9isexpressedinglialcelllineagesinthedevelopingmousespinalcord AT tuckerashley transcriptionfactorhb9isexpressedinglialcelllineagesinthedevelopingmousespinalcord AT mirandadiego transcriptionfactorhb9isexpressedinglialcelllineagesinthedevelopingmousespinalcord AT adkinsrobertl transcriptionfactorhb9isexpressedinglialcelllineagesinthedevelopingmousespinalcord AT acevesmiriam transcriptionfactorhb9isexpressedinglialcelllineagesinthedevelopingmousespinalcord AT dietzvalerie transcriptionfactorhb9isexpressedinglialcelllineagesinthedevelopingmousespinalcord AT jagritvipin transcriptionfactorhb9isexpressedinglialcelllineagesinthedevelopingmousespinalcord AT leonardsamy transcriptionfactorhb9isexpressedinglialcelllineagesinthedevelopingmousespinalcord AT leeyoungil transcriptionfactorhb9isexpressedinglialcelllineagesinthedevelopingmousespinalcord AT dulinjennifern transcriptionfactorhb9isexpressedinglialcelllineagesinthedevelopingmousespinalcord |