Cargando…

Pyrroloquinoline Quinone Administration Alleviates Allergic Airway Inflammation in Mice by Regulating the JAK-STAT Signaling Pathway

The current asthma therapies are inadequate for many patients with severe asthma. Pyrroloquinoline quinone (PQQ) is a naturally-occurring redox cofactor and nutrient that can exert a multitude of physiological effects, including anti-inflammatory and antioxidative effects. We sought to explore the e...

Descripción completa

Detalles Bibliográficos
Autores principales: Min, Zhihui, Zhou, Jiebai, Mao, Ruolin, Cui, Bo, Cheng, Yunfeng, Chen, Zhihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9637035/
https://www.ncbi.nlm.nih.gov/pubmed/36345503
http://dx.doi.org/10.1155/2022/1267841
_version_ 1784825086037983232
author Min, Zhihui
Zhou, Jiebai
Mao, Ruolin
Cui, Bo
Cheng, Yunfeng
Chen, Zhihong
author_facet Min, Zhihui
Zhou, Jiebai
Mao, Ruolin
Cui, Bo
Cheng, Yunfeng
Chen, Zhihong
author_sort Min, Zhihui
collection PubMed
description The current asthma therapies are inadequate for many patients with severe asthma. Pyrroloquinoline quinone (PQQ) is a naturally-occurring redox cofactor and nutrient that can exert a multitude of physiological effects, including anti-inflammatory and antioxidative effects. We sought to explore the effects of PQQ on allergic airway inflammation and reveal the underlying mechanisms. In vitro, the effects of PQQ on the secretion of epithelial-derived cytokines by house dust mite- (HDM-) incubated 16-HBE cells and on the differentiation potential of CD4+ T cells were investigated. In vivo, PQQ was administered to mice with ovalbumin- (OVA-) induced asthma, and lung pathology and inflammatory cell infiltration were assessed. The changes in T cell subsets and signal transducers and activators of transcription (STATs) were evaluated by flow cytometry. Pretreatment with PQQ significantly decreased HDM-stimulated thymic stromal lymphopoietin (TSLP) production in a dose-dependent manner in 16-HBE cells and inhibited Th2 cell differentiation in vitro. Treatment with PQQ significantly reduced bronchoalveolar lavage fluid (BALF) inflammatory cell counts in the OVA-induced mouse model. PQQ administration also changed the secretion of IFN-γ and IL-4 as well as the percentages of Th1, Th2, Th17, and Treg cells in the peripheral blood and lung tissues, along with inhibition the phosphorylation of STAT1, STAT3, and STAT6 while promoting that of STAT4 in allergic airway inflammation model mice. PQQ can alleviate allergic airway inflammation in mice by improving the immune microenvironment and regulating the JAK-STAT signaling pathway. Our findings suggest that PQQ has great potential as a novel therapeutic agent for inflammatory diseases, including asthma.
format Online
Article
Text
id pubmed-9637035
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-96370352022-11-06 Pyrroloquinoline Quinone Administration Alleviates Allergic Airway Inflammation in Mice by Regulating the JAK-STAT Signaling Pathway Min, Zhihui Zhou, Jiebai Mao, Ruolin Cui, Bo Cheng, Yunfeng Chen, Zhihong Mediators Inflamm Research Article The current asthma therapies are inadequate for many patients with severe asthma. Pyrroloquinoline quinone (PQQ) is a naturally-occurring redox cofactor and nutrient that can exert a multitude of physiological effects, including anti-inflammatory and antioxidative effects. We sought to explore the effects of PQQ on allergic airway inflammation and reveal the underlying mechanisms. In vitro, the effects of PQQ on the secretion of epithelial-derived cytokines by house dust mite- (HDM-) incubated 16-HBE cells and on the differentiation potential of CD4+ T cells were investigated. In vivo, PQQ was administered to mice with ovalbumin- (OVA-) induced asthma, and lung pathology and inflammatory cell infiltration were assessed. The changes in T cell subsets and signal transducers and activators of transcription (STATs) were evaluated by flow cytometry. Pretreatment with PQQ significantly decreased HDM-stimulated thymic stromal lymphopoietin (TSLP) production in a dose-dependent manner in 16-HBE cells and inhibited Th2 cell differentiation in vitro. Treatment with PQQ significantly reduced bronchoalveolar lavage fluid (BALF) inflammatory cell counts in the OVA-induced mouse model. PQQ administration also changed the secretion of IFN-γ and IL-4 as well as the percentages of Th1, Th2, Th17, and Treg cells in the peripheral blood and lung tissues, along with inhibition the phosphorylation of STAT1, STAT3, and STAT6 while promoting that of STAT4 in allergic airway inflammation model mice. PQQ can alleviate allergic airway inflammation in mice by improving the immune microenvironment and regulating the JAK-STAT signaling pathway. Our findings suggest that PQQ has great potential as a novel therapeutic agent for inflammatory diseases, including asthma. Hindawi 2022-10-29 /pmc/articles/PMC9637035/ /pubmed/36345503 http://dx.doi.org/10.1155/2022/1267841 Text en Copyright © 2022 Zhihui Min et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Min, Zhihui
Zhou, Jiebai
Mao, Ruolin
Cui, Bo
Cheng, Yunfeng
Chen, Zhihong
Pyrroloquinoline Quinone Administration Alleviates Allergic Airway Inflammation in Mice by Regulating the JAK-STAT Signaling Pathway
title Pyrroloquinoline Quinone Administration Alleviates Allergic Airway Inflammation in Mice by Regulating the JAK-STAT Signaling Pathway
title_full Pyrroloquinoline Quinone Administration Alleviates Allergic Airway Inflammation in Mice by Regulating the JAK-STAT Signaling Pathway
title_fullStr Pyrroloquinoline Quinone Administration Alleviates Allergic Airway Inflammation in Mice by Regulating the JAK-STAT Signaling Pathway
title_full_unstemmed Pyrroloquinoline Quinone Administration Alleviates Allergic Airway Inflammation in Mice by Regulating the JAK-STAT Signaling Pathway
title_short Pyrroloquinoline Quinone Administration Alleviates Allergic Airway Inflammation in Mice by Regulating the JAK-STAT Signaling Pathway
title_sort pyrroloquinoline quinone administration alleviates allergic airway inflammation in mice by regulating the jak-stat signaling pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9637035/
https://www.ncbi.nlm.nih.gov/pubmed/36345503
http://dx.doi.org/10.1155/2022/1267841
work_keys_str_mv AT minzhihui pyrroloquinolinequinoneadministrationalleviatesallergicairwayinflammationinmicebyregulatingthejakstatsignalingpathway
AT zhoujiebai pyrroloquinolinequinoneadministrationalleviatesallergicairwayinflammationinmicebyregulatingthejakstatsignalingpathway
AT maoruolin pyrroloquinolinequinoneadministrationalleviatesallergicairwayinflammationinmicebyregulatingthejakstatsignalingpathway
AT cuibo pyrroloquinolinequinoneadministrationalleviatesallergicairwayinflammationinmicebyregulatingthejakstatsignalingpathway
AT chengyunfeng pyrroloquinolinequinoneadministrationalleviatesallergicairwayinflammationinmicebyregulatingthejakstatsignalingpathway
AT chenzhihong pyrroloquinolinequinoneadministrationalleviatesallergicairwayinflammationinmicebyregulatingthejakstatsignalingpathway