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Serum 25-hydroxycholesterol levels are increased in patients with coronavirus disease 2019()

BACKGROUND: 25-hydroxycholesterol (25HC), produced by cholesterol 25-hydroxylase (CH25H) in macrophages, has been reported to inhibit the replication of viral pathogens such as severe acute respiratory syndrome coronavirus-2. Also, CH25H expression in macrophages is robustly induced by interferons (...

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Detalles Bibliográficos
Autores principales: Asano, Takumi, Wakabayashi, Tetsuji, Kondo, Yasuyuki, Okada, Kenta, Yamamuro, Daisuke, Koga, Yukiko, Oka, Kiyonori, Sakurai, Momoe, Sawayama, Nagisa, Takahashi, Manabu, Okazaki, Hiroaki, Ebihara, Ken, Minami, Kensuke, Morisawa, Yuji, Hatakeyama, Shuji, Matsumura, Masami, Ishibashi, Shun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Lipid Association. Published by Elsevier Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9637049/
https://www.ncbi.nlm.nih.gov/pubmed/36522261
http://dx.doi.org/10.1016/j.jacl.2022.10.012
Descripción
Sumario:BACKGROUND: 25-hydroxycholesterol (25HC), produced by cholesterol 25-hydroxylase (CH25H) in macrophages, has been reported to inhibit the replication of viral pathogens such as severe acute respiratory syndrome coronavirus-2. Also, CH25H expression in macrophages is robustly induced by interferons (IFNs). OBJECTIVE: To better understand the serum level increase of 25HC in coronavirus disease 2019 (COVID-19) and how it relates to the clinical picture. METHODS: We measured the serum levels of 25HC and five other oxysterols in 17 hospitalized COVID-19 patients. RESULTS: On admission, 25HC and 27-hydroxycholesterol (27HC) serum levels were elevated; however, 7-ketocholesterol (7KC) levels were lower in patients with COVID-19 than in the healthy controls. There was no significant correlation between 25HC serum levels and disease severity markers, such as interferon-gamma (IFN-γ) and interleukin 6. Dexamethasone effectively suppressed cholesterol 25-hydroxylase (CH25H) mRNA expression in RAW 264.7 cells, a murine leukemia macrophage cell line, with or without lipopolysaccharide or IFNs; therefore, it might mitigate the increasing effects of COVID-19 on the serum levels of 25HC. CONCLUSIONS: Our results highlighted that 25HC could be used as a unique biomarker in severe COVID-19 and a potential therapeutic candidate for detecting the severity of COVID-19 and other infectious diseases.