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Metabolite profiling of tubers of an early- and a late-maturing potato line and their grafts

INTRODUCTION: Earliness of tuberisation and the quality of potato tubers are important traits in potato breeding. The qualitative traits rely on the metabolite profile of tubers, which are storage organs and net importers of assimilates. Thus, the quality of tubers largely depends on the metabolites...

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Autores principales: Villányi, Vanda, Gondor, Orsolya Kinga, Bánfalvi, Zsófia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9637070/
https://www.ncbi.nlm.nih.gov/pubmed/36334159
http://dx.doi.org/10.1007/s11306-022-01950-3
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author Villányi, Vanda
Gondor, Orsolya Kinga
Bánfalvi, Zsófia
author_facet Villányi, Vanda
Gondor, Orsolya Kinga
Bánfalvi, Zsófia
author_sort Villányi, Vanda
collection PubMed
description INTRODUCTION: Earliness of tuberisation and the quality of potato tubers are important traits in potato breeding. The qualitative traits rely on the metabolite profile of tubers, which are storage organs and net importers of assimilates. Thus, the quality of tubers largely depends on the metabolites transported from leaves to developing tubers. OBJECTIVES: To test the influence of canopy on the quality of tubers by metabolite profiling of tubers of an early- and a late-maturing potato line and their grafts. METHODS: Potatoes were grown under greenhouse conditions, grafted and the tubers harvested at the end of the scions’ vegetation period. Metabolite profiling of freshly harvested tubers was performed using gas chromatography coupled with mass spectrometry. Statistical analyses were applied to determine the significant differences between the different tubers. RESULTS: 99 metabolites were identified and an additional 181 peaks detected in chromatograms, out of which 186 were polar and 94 non-polar compounds. The concentrations of 113 metabolites were significantly different in the tubers from the early-maturing CE3130 and the late-maturing CE3027 line. Hetero-grafting resulted in considerable changes in the metabolite content of tubers. Especially, the effect of CE3027 on the metabolite composition of tubers formed on CE3130 rootstocks was readily apparent. Nevertheless, many compounds were present at similar levels in the tubers of hetero-grafted plants as was found in the tubers of their scion counterparts. CONCLUSION: Hetero-grafting resulted in many compounds at similar concentrations in rootstock tubers as in scion tubers suggesting that these are transported from the source leaves to tubers. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11306-022-01950-3.
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spelling pubmed-96370702022-11-07 Metabolite profiling of tubers of an early- and a late-maturing potato line and their grafts Villányi, Vanda Gondor, Orsolya Kinga Bánfalvi, Zsófia Metabolomics Original Article INTRODUCTION: Earliness of tuberisation and the quality of potato tubers are important traits in potato breeding. The qualitative traits rely on the metabolite profile of tubers, which are storage organs and net importers of assimilates. Thus, the quality of tubers largely depends on the metabolites transported from leaves to developing tubers. OBJECTIVES: To test the influence of canopy on the quality of tubers by metabolite profiling of tubers of an early- and a late-maturing potato line and their grafts. METHODS: Potatoes were grown under greenhouse conditions, grafted and the tubers harvested at the end of the scions’ vegetation period. Metabolite profiling of freshly harvested tubers was performed using gas chromatography coupled with mass spectrometry. Statistical analyses were applied to determine the significant differences between the different tubers. RESULTS: 99 metabolites were identified and an additional 181 peaks detected in chromatograms, out of which 186 were polar and 94 non-polar compounds. The concentrations of 113 metabolites were significantly different in the tubers from the early-maturing CE3130 and the late-maturing CE3027 line. Hetero-grafting resulted in considerable changes in the metabolite content of tubers. Especially, the effect of CE3027 on the metabolite composition of tubers formed on CE3130 rootstocks was readily apparent. Nevertheless, many compounds were present at similar levels in the tubers of hetero-grafted plants as was found in the tubers of their scion counterparts. CONCLUSION: Hetero-grafting resulted in many compounds at similar concentrations in rootstock tubers as in scion tubers suggesting that these are transported from the source leaves to tubers. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11306-022-01950-3. Springer US 2022-11-05 2022 /pmc/articles/PMC9637070/ /pubmed/36334159 http://dx.doi.org/10.1007/s11306-022-01950-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Villányi, Vanda
Gondor, Orsolya Kinga
Bánfalvi, Zsófia
Metabolite profiling of tubers of an early- and a late-maturing potato line and their grafts
title Metabolite profiling of tubers of an early- and a late-maturing potato line and their grafts
title_full Metabolite profiling of tubers of an early- and a late-maturing potato line and their grafts
title_fullStr Metabolite profiling of tubers of an early- and a late-maturing potato line and their grafts
title_full_unstemmed Metabolite profiling of tubers of an early- and a late-maturing potato line and their grafts
title_short Metabolite profiling of tubers of an early- and a late-maturing potato line and their grafts
title_sort metabolite profiling of tubers of an early- and a late-maturing potato line and their grafts
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9637070/
https://www.ncbi.nlm.nih.gov/pubmed/36334159
http://dx.doi.org/10.1007/s11306-022-01950-3
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