Cargando…
The Effect of Gracilaria Corticata and Scenedesmus Acuminates Extract Mixture on the Healing of Wounds Contaminated with Staphylococcus in the Rat Model
INTRODUCTION: Wound healing processes are dependent on the severity of the trauma, invasion of opportunistic microorganisms, and inflammatory, immunological, and metabolic responses. We tried to show the ability of algae to inhibit wound infection, which can lead to proper wound healing. METHODS: Ei...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shahid Beheshti University of Medical Sciences
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9637266/ https://www.ncbi.nlm.nih.gov/pubmed/36381975 http://dx.doi.org/10.22037/aaem.v10i1.1686 |
_version_ | 1784825146864828416 |
---|---|
author | Akasheh, Hooman Jahandideh, Alireza Khajerahimi, Amireghbal Kakoolaki, Shapour Hesaraki, Saeed |
author_facet | Akasheh, Hooman Jahandideh, Alireza Khajerahimi, Amireghbal Kakoolaki, Shapour Hesaraki, Saeed |
author_sort | Akasheh, Hooman |
collection | PubMed |
description | INTRODUCTION: Wound healing processes are dependent on the severity of the trauma, invasion of opportunistic microorganisms, and inflammatory, immunological, and metabolic responses. We tried to show the ability of algae to inhibit wound infection, which can lead to proper wound healing. METHODS: Eighty rats were housed according to laboratory animal care protocols and divided into four groups at each operating time. Group I consisted of the non-treated animals. Group II was treated with 25% zinc oxide as a choice treatment. In the treated groups 3 and 4, an equal ratio of Gracilaria Corticata and Scenedesmus acuminate marine algae (mixed algae) was applied as 3% and 7% ointment pomade. Percentage of wound closure, number of bacteria in the wound surface, angiogenesis (Vascular endothelial growth factor; VEGF), the number of macrophages, collagen production level and transforming growth factor-beta (TGFβ), epithelialization, and fibrosis were evaluated. RESULTS: Applying mixed algae extract 7% and zinc oxide 25% could result in a mild improvement in wound closure (df: 9, 48; F=5.97; p<0.0001). In addition, mixed algae 3%, mixed algae 7% and zinc oxide could reduce the rate of bacterial growth compared to non-treated animals (df: 3, 16; F=5.74; p=0.0007). However, these improvements do not seem to be clinically significant. Induction of angiogenesis, increase in macrophage infiltration rate, and expression of TGFβ are possible underlying mechanisms of mixed algae in accelerating wound healing process. CONCLUSION: The result showed that the administration of 3% and 7% mixed algae could mildly accelerate the wound healing process in a rat model of pelleted skin wound. However, it seems that its effect is not clinically significant compared to non-treated and zinc oxide treated animals. |
format | Online Article Text |
id | pubmed-9637266 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Shahid Beheshti University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-96372662022-11-14 The Effect of Gracilaria Corticata and Scenedesmus Acuminates Extract Mixture on the Healing of Wounds Contaminated with Staphylococcus in the Rat Model Akasheh, Hooman Jahandideh, Alireza Khajerahimi, Amireghbal Kakoolaki, Shapour Hesaraki, Saeed Arch Acad Emerg Med Original Research Article INTRODUCTION: Wound healing processes are dependent on the severity of the trauma, invasion of opportunistic microorganisms, and inflammatory, immunological, and metabolic responses. We tried to show the ability of algae to inhibit wound infection, which can lead to proper wound healing. METHODS: Eighty rats were housed according to laboratory animal care protocols and divided into four groups at each operating time. Group I consisted of the non-treated animals. Group II was treated with 25% zinc oxide as a choice treatment. In the treated groups 3 and 4, an equal ratio of Gracilaria Corticata and Scenedesmus acuminate marine algae (mixed algae) was applied as 3% and 7% ointment pomade. Percentage of wound closure, number of bacteria in the wound surface, angiogenesis (Vascular endothelial growth factor; VEGF), the number of macrophages, collagen production level and transforming growth factor-beta (TGFβ), epithelialization, and fibrosis were evaluated. RESULTS: Applying mixed algae extract 7% and zinc oxide 25% could result in a mild improvement in wound closure (df: 9, 48; F=5.97; p<0.0001). In addition, mixed algae 3%, mixed algae 7% and zinc oxide could reduce the rate of bacterial growth compared to non-treated animals (df: 3, 16; F=5.74; p=0.0007). However, these improvements do not seem to be clinically significant. Induction of angiogenesis, increase in macrophage infiltration rate, and expression of TGFβ are possible underlying mechanisms of mixed algae in accelerating wound healing process. CONCLUSION: The result showed that the administration of 3% and 7% mixed algae could mildly accelerate the wound healing process in a rat model of pelleted skin wound. However, it seems that its effect is not clinically significant compared to non-treated and zinc oxide treated animals. Shahid Beheshti University of Medical Sciences 2022-08-29 /pmc/articles/PMC9637266/ /pubmed/36381975 http://dx.doi.org/10.22037/aaem.v10i1.1686 Text en https://creativecommons.org/licenses/by-nc/3.0/This open-access article distributed under the terms of the Creative Commons Attribution NonCommercial 3.0 License (CC BY-NC 3.0). (https://creativecommons.org/licenses/by-nc/3.0/) |
spellingShingle | Original Research Article Akasheh, Hooman Jahandideh, Alireza Khajerahimi, Amireghbal Kakoolaki, Shapour Hesaraki, Saeed The Effect of Gracilaria Corticata and Scenedesmus Acuminates Extract Mixture on the Healing of Wounds Contaminated with Staphylococcus in the Rat Model |
title | The Effect of Gracilaria Corticata and Scenedesmus Acuminates Extract Mixture on the Healing of Wounds Contaminated with Staphylococcus in the Rat Model |
title_full | The Effect of Gracilaria Corticata and Scenedesmus Acuminates Extract Mixture on the Healing of Wounds Contaminated with Staphylococcus in the Rat Model |
title_fullStr | The Effect of Gracilaria Corticata and Scenedesmus Acuminates Extract Mixture on the Healing of Wounds Contaminated with Staphylococcus in the Rat Model |
title_full_unstemmed | The Effect of Gracilaria Corticata and Scenedesmus Acuminates Extract Mixture on the Healing of Wounds Contaminated with Staphylococcus in the Rat Model |
title_short | The Effect of Gracilaria Corticata and Scenedesmus Acuminates Extract Mixture on the Healing of Wounds Contaminated with Staphylococcus in the Rat Model |
title_sort | effect of gracilaria corticata and scenedesmus acuminates extract mixture on the healing of wounds contaminated with staphylococcus in the rat model |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9637266/ https://www.ncbi.nlm.nih.gov/pubmed/36381975 http://dx.doi.org/10.22037/aaem.v10i1.1686 |
work_keys_str_mv | AT akashehhooman theeffectofgracilariacorticataandscenedesmusacuminatesextractmixtureonthehealingofwoundscontaminatedwithstaphylococcusintheratmodel AT jahandidehalireza theeffectofgracilariacorticataandscenedesmusacuminatesextractmixtureonthehealingofwoundscontaminatedwithstaphylococcusintheratmodel AT khajerahimiamireghbal theeffectofgracilariacorticataandscenedesmusacuminatesextractmixtureonthehealingofwoundscontaminatedwithstaphylococcusintheratmodel AT kakoolakishapour theeffectofgracilariacorticataandscenedesmusacuminatesextractmixtureonthehealingofwoundscontaminatedwithstaphylococcusintheratmodel AT hesarakisaeed theeffectofgracilariacorticataandscenedesmusacuminatesextractmixtureonthehealingofwoundscontaminatedwithstaphylococcusintheratmodel AT akashehhooman effectofgracilariacorticataandscenedesmusacuminatesextractmixtureonthehealingofwoundscontaminatedwithstaphylococcusintheratmodel AT jahandidehalireza effectofgracilariacorticataandscenedesmusacuminatesextractmixtureonthehealingofwoundscontaminatedwithstaphylococcusintheratmodel AT khajerahimiamireghbal effectofgracilariacorticataandscenedesmusacuminatesextractmixtureonthehealingofwoundscontaminatedwithstaphylococcusintheratmodel AT kakoolakishapour effectofgracilariacorticataandscenedesmusacuminatesextractmixtureonthehealingofwoundscontaminatedwithstaphylococcusintheratmodel AT hesarakisaeed effectofgracilariacorticataandscenedesmusacuminatesextractmixtureonthehealingofwoundscontaminatedwithstaphylococcusintheratmodel |