Cargando…

Aging hampers neutrophil extracellular traps (NETs) efficacy

BACKGROUND: NETosis is a neutrophil-mediated defense mechanism during which DNA and enzymes are extruded forming a network (NETs) trapping and killing different pathogens. NETosis is reduced in both mice and humans during aging. AIMS: We explored the difference in the efficacy of NETs released in el...

Descripción completa

Detalles Bibliográficos
Autores principales: Sabbatini, Maurizio, Bona, Elisa, Novello, Giorgia, Migliario, Mario, Renò, Filippo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9637667/
https://www.ncbi.nlm.nih.gov/pubmed/35920993
http://dx.doi.org/10.1007/s40520-022-02201-0
_version_ 1784825237967208448
author Sabbatini, Maurizio
Bona, Elisa
Novello, Giorgia
Migliario, Mario
Renò, Filippo
author_facet Sabbatini, Maurizio
Bona, Elisa
Novello, Giorgia
Migliario, Mario
Renò, Filippo
author_sort Sabbatini, Maurizio
collection PubMed
description BACKGROUND: NETosis is a neutrophil-mediated defense mechanism during which DNA and enzymes are extruded forming a network (NETs) trapping and killing different pathogens. NETosis is reduced in both mice and humans during aging. AIMS: We explored the difference in the efficacy of NETs released in elderly (> 65 years) versus adults (20–50 years) subjects in inhibiting Staphylococcus aureus growth and activating the growth of keratinocytes. METHODS: Neutrophil granulocytes, obtained from venous blood both in healthy elderly and adult subjects, were stimulated by LPS (0–250 µg/ml) to induce the formation of NET. NETs were quantified by SYBR Green staining and growth inhibition of S. aureus was evaluated by disk diffusion test. Furthermore, NETs (0–500 ng/ml) were added to immortalized human keratinocytes (HaCaT cells), and their proliferation was evaluated by MTT assay after 24 h. Finally, the DNA size of NETs was evaluated by flow cytometry after SYBR Green staining. RESULTS: Greater production of NETs was observed in elderly subjects than in adults, but these NETs showed reduced bactericidal capacity and HaCaT cells’ proliferation stimulation. The activities of the NETs are related to the size of the extruded DNA threads, and when NETs size was analyzed, DNA from elderly showed a higher size compared to that obtained by adults. DISCUSSION: Unexpected results showed aging-related NETs structural modification resulting in both a lower antimicrobial activity and keratinocyte proliferation stimulation compared to NETs obtained from adults. CONCLUSIONS: The NETs DNA size observed in elderly subjects has not been previously reported and could be part of other pathogenic mechanisms observed in aging.
format Online
Article
Text
id pubmed-9637667
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Springer International Publishing
record_format MEDLINE/PubMed
spelling pubmed-96376672022-11-08 Aging hampers neutrophil extracellular traps (NETs) efficacy Sabbatini, Maurizio Bona, Elisa Novello, Giorgia Migliario, Mario Renò, Filippo Aging Clin Exp Res Original Article BACKGROUND: NETosis is a neutrophil-mediated defense mechanism during which DNA and enzymes are extruded forming a network (NETs) trapping and killing different pathogens. NETosis is reduced in both mice and humans during aging. AIMS: We explored the difference in the efficacy of NETs released in elderly (> 65 years) versus adults (20–50 years) subjects in inhibiting Staphylococcus aureus growth and activating the growth of keratinocytes. METHODS: Neutrophil granulocytes, obtained from venous blood both in healthy elderly and adult subjects, were stimulated by LPS (0–250 µg/ml) to induce the formation of NET. NETs were quantified by SYBR Green staining and growth inhibition of S. aureus was evaluated by disk diffusion test. Furthermore, NETs (0–500 ng/ml) were added to immortalized human keratinocytes (HaCaT cells), and their proliferation was evaluated by MTT assay after 24 h. Finally, the DNA size of NETs was evaluated by flow cytometry after SYBR Green staining. RESULTS: Greater production of NETs was observed in elderly subjects than in adults, but these NETs showed reduced bactericidal capacity and HaCaT cells’ proliferation stimulation. The activities of the NETs are related to the size of the extruded DNA threads, and when NETs size was analyzed, DNA from elderly showed a higher size compared to that obtained by adults. DISCUSSION: Unexpected results showed aging-related NETs structural modification resulting in both a lower antimicrobial activity and keratinocyte proliferation stimulation compared to NETs obtained from adults. CONCLUSIONS: The NETs DNA size observed in elderly subjects has not been previously reported and could be part of other pathogenic mechanisms observed in aging. Springer International Publishing 2022-08-03 2022 /pmc/articles/PMC9637667/ /pubmed/35920993 http://dx.doi.org/10.1007/s40520-022-02201-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Sabbatini, Maurizio
Bona, Elisa
Novello, Giorgia
Migliario, Mario
Renò, Filippo
Aging hampers neutrophil extracellular traps (NETs) efficacy
title Aging hampers neutrophil extracellular traps (NETs) efficacy
title_full Aging hampers neutrophil extracellular traps (NETs) efficacy
title_fullStr Aging hampers neutrophil extracellular traps (NETs) efficacy
title_full_unstemmed Aging hampers neutrophil extracellular traps (NETs) efficacy
title_short Aging hampers neutrophil extracellular traps (NETs) efficacy
title_sort aging hampers neutrophil extracellular traps (nets) efficacy
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9637667/
https://www.ncbi.nlm.nih.gov/pubmed/35920993
http://dx.doi.org/10.1007/s40520-022-02201-0
work_keys_str_mv AT sabbatinimaurizio aginghampersneutrophilextracellulartrapsnetsefficacy
AT bonaelisa aginghampersneutrophilextracellulartrapsnetsefficacy
AT novellogiorgia aginghampersneutrophilextracellulartrapsnetsefficacy
AT migliariomario aginghampersneutrophilextracellulartrapsnetsefficacy
AT renofilippo aginghampersneutrophilextracellulartrapsnetsefficacy