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Dissection of murine collecting lymphatic vessels for imaging, single-cell analysis, and tissue culture of lymphatic muscle cells

In vivo and in vitro systems have the potential to provide a framework to study the organization, gene expression, and functionality of lymphatic and blood vessel smooth muscle cells in physiology and disease settings. A series of procedures are described here, including the surgical isolation of mo...

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Detalles Bibliográficos
Autores principales: Arroyo-Ataz, Guillermo, Jones, Dennis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9637822/
https://www.ncbi.nlm.nih.gov/pubmed/36353362
http://dx.doi.org/10.1016/j.xpro.2022.101800
Descripción
Sumario:In vivo and in vitro systems have the potential to provide a framework to study the organization, gene expression, and functionality of lymphatic and blood vessel smooth muscle cells in physiology and disease settings. A series of procedures are described here, including the surgical isolation of mouse collecting lymphatic vessels and blood vessels, whole-mount immunofluorescence staining of muscle cells on the vessels, and the enzymatic digestion and culture of α- smooth muscle actin+ cells from the vessels. For complete details on the use and execution of this protocol, please refer to Jones et al. (2018).