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Context-dependent differences in the functional responses of Lactobacillaceae strains to fermentable sugars

Lactobacillaceae are Gram-positive rods, facultative anaerobes, and belong to the lactic acid bacteria (LAB) that frequently serve as probiotics. We systematically compared five LAB strains for the effects of different carbohydrates on their free-living and biofilm lifestyles. We found that fermenta...

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Autores principales: Suissa, Ronit, Oved, Rela, Maan, Harsh, Hadad, Uzi, Gilhar, Omri, Meijler, Michael M., Koren, Omry, Kolodkin-Gal, Ilana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9637956/
https://www.ncbi.nlm.nih.gov/pubmed/36353463
http://dx.doi.org/10.3389/fmicb.2022.949932
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author Suissa, Ronit
Oved, Rela
Maan, Harsh
Hadad, Uzi
Gilhar, Omri
Meijler, Michael M.
Koren, Omry
Kolodkin-Gal, Ilana
author_facet Suissa, Ronit
Oved, Rela
Maan, Harsh
Hadad, Uzi
Gilhar, Omri
Meijler, Michael M.
Koren, Omry
Kolodkin-Gal, Ilana
author_sort Suissa, Ronit
collection PubMed
description Lactobacillaceae are Gram-positive rods, facultative anaerobes, and belong to the lactic acid bacteria (LAB) that frequently serve as probiotics. We systematically compared five LAB strains for the effects of different carbohydrates on their free-living and biofilm lifestyles. We found that fermentable sugars triggered an altered carrying capacity with strain specificity during planktonic growth. In addition, heterogeneous response to fermentable sugar was manifested in microbial aggregation (measured by imaging flow cytometry), colony development, and attachment to mucin. The acid production capacities of the strains were compatible and could not account for heterogeneity in their differential carrying capacity in liquid and on a solid medium. Among tested LAB strains, L. paracasei, and L. rhamnosus GG survived self-imposed acid stress while L. acidophilus was extremely sensitive to its own glucose utilization acidic products. The addition of a buffering system during growth on a solid medium significantly improved the survival of most tested probiotic strains during fermentation, but the formation of biofilms and aggregation capacity were responsive to the carbohydrate provided rather than to the acidity. We suggest that the optimal performance of the beneficial microbiota members belonging to Lactobacillaceae varies as a function of the growth model and the dependency on a buffering system.
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spelling pubmed-96379562022-11-08 Context-dependent differences in the functional responses of Lactobacillaceae strains to fermentable sugars Suissa, Ronit Oved, Rela Maan, Harsh Hadad, Uzi Gilhar, Omri Meijler, Michael M. Koren, Omry Kolodkin-Gal, Ilana Front Microbiol Microbiology Lactobacillaceae are Gram-positive rods, facultative anaerobes, and belong to the lactic acid bacteria (LAB) that frequently serve as probiotics. We systematically compared five LAB strains for the effects of different carbohydrates on their free-living and biofilm lifestyles. We found that fermentable sugars triggered an altered carrying capacity with strain specificity during planktonic growth. In addition, heterogeneous response to fermentable sugar was manifested in microbial aggregation (measured by imaging flow cytometry), colony development, and attachment to mucin. The acid production capacities of the strains were compatible and could not account for heterogeneity in their differential carrying capacity in liquid and on a solid medium. Among tested LAB strains, L. paracasei, and L. rhamnosus GG survived self-imposed acid stress while L. acidophilus was extremely sensitive to its own glucose utilization acidic products. The addition of a buffering system during growth on a solid medium significantly improved the survival of most tested probiotic strains during fermentation, but the formation of biofilms and aggregation capacity were responsive to the carbohydrate provided rather than to the acidity. We suggest that the optimal performance of the beneficial microbiota members belonging to Lactobacillaceae varies as a function of the growth model and the dependency on a buffering system. Frontiers Media S.A. 2022-10-24 /pmc/articles/PMC9637956/ /pubmed/36353463 http://dx.doi.org/10.3389/fmicb.2022.949932 Text en Copyright © 2022 Suissa, Oved, Maan, Hadad, Gilhar, Meijler, Koren and Kolodkin-Gal. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Suissa, Ronit
Oved, Rela
Maan, Harsh
Hadad, Uzi
Gilhar, Omri
Meijler, Michael M.
Koren, Omry
Kolodkin-Gal, Ilana
Context-dependent differences in the functional responses of Lactobacillaceae strains to fermentable sugars
title Context-dependent differences in the functional responses of Lactobacillaceae strains to fermentable sugars
title_full Context-dependent differences in the functional responses of Lactobacillaceae strains to fermentable sugars
title_fullStr Context-dependent differences in the functional responses of Lactobacillaceae strains to fermentable sugars
title_full_unstemmed Context-dependent differences in the functional responses of Lactobacillaceae strains to fermentable sugars
title_short Context-dependent differences in the functional responses of Lactobacillaceae strains to fermentable sugars
title_sort context-dependent differences in the functional responses of lactobacillaceae strains to fermentable sugars
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9637956/
https://www.ncbi.nlm.nih.gov/pubmed/36353463
http://dx.doi.org/10.3389/fmicb.2022.949932
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