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Marker assisted improvement for leaf rust and moisture deficit stress tolerance in wheat variety HD3086

There is a significant yield reduction in the wheat crop as a result of different biotic and abiotic stresses, and changing climate, among them moisture deficit stress and leaf rust are the major ones affecting wheat worldwide. HD3086 is a high-yielding wheat variety that has been released for comme...

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Autores principales: Sunilkumar, V.P., Krishna, Hari, Devate, Narayana Bhat, Manjunath, Karthik Kumar, Chauhan, Divya, Singh, Shweta, Sinha, Nivedita, Singh, Jang Bahadur, Prakasha, T. L., Pal, Dharam, Sivasamy, M., Jain, Neelu, Singh, G. P., Singh, P. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9638138/
https://www.ncbi.nlm.nih.gov/pubmed/36352858
http://dx.doi.org/10.3389/fpls.2022.1035016
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author Sunilkumar, V.P.
Krishna, Hari
Devate, Narayana Bhat
Manjunath, Karthik Kumar
Chauhan, Divya
Singh, Shweta
Sinha, Nivedita
Singh, Jang Bahadur
Prakasha, T. L.
Pal, Dharam
Sivasamy, M.
Jain, Neelu
Singh, G. P.
Singh, P. K.
author_facet Sunilkumar, V.P.
Krishna, Hari
Devate, Narayana Bhat
Manjunath, Karthik Kumar
Chauhan, Divya
Singh, Shweta
Sinha, Nivedita
Singh, Jang Bahadur
Prakasha, T. L.
Pal, Dharam
Sivasamy, M.
Jain, Neelu
Singh, G. P.
Singh, P. K.
author_sort Sunilkumar, V.P.
collection PubMed
description There is a significant yield reduction in the wheat crop as a result of different biotic and abiotic stresses, and changing climate, among them moisture deficit stress and leaf rust are the major ones affecting wheat worldwide. HD3086 is a high-yielding wheat variety that has been released for commercial cultivation under timely sown irrigated conditions in the Indo-Gangetic plains of India. Variety HD3086 provides a good, stable yield, and it is the choice of millions of farmers in India. It becomes susceptible to the most prevalent pathotypes 77-5 and 77-9 of Puccinia triticina (causing leaf rust) in the production environment and its potential yield cannot be realized under moisture deficit stress. The present study demonstrates the use of a marker-assisted back cross breeding approach to the successful transfer of leaf rust resistance gene Lr24 and QTLs linked to moisture deficit stress tolerance in the background of HD3086. The genotype HI1500 was used as a donor parent that possesses leaf rust-resistant gene Lr24, which confers resistance against the major pathotypes found in the production environment. It possesses inbuilt tolerance under abiotic stresses with superior quality traits. Foreground selection for gene Lr24 and moisture deficit stress tolerance QTLs linked to Canopy temperature (CT), Normal Differential Vegetation Index (NDVI) and Thousand Kernel Weight (TKW) in different generations of the backcrossing and selection. In BC2F2, foreground selection was carried out to identify homozygous lines based on the linked markers and were advanced following pedigree based phenotypic selection. The selected lines were evaluated against P. triticina pathotypes 77-5 and 77-9 under controlled conditions. Recurrent parent recovery of the selected lines ranged from 78-94%. The identified lines were evaluated for their tolerance to moisture stress under field conditions and their resistance to rust under artificial epiphytotic conditions for two years. In BC2F5 generation, eight positive lines for marker alleles were selected which showed resistance to leaf rust and recorded an improvement in component traits of moisture deficit stress tolerance such as CT, NDVI, TKW and yield compared to the recurrent parent HD3086. The derived line is named HD3471 and is nominated for national trials for testing and further release for commercial cultivation.
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spelling pubmed-96381382022-11-08 Marker assisted improvement for leaf rust and moisture deficit stress tolerance in wheat variety HD3086 Sunilkumar, V.P. Krishna, Hari Devate, Narayana Bhat Manjunath, Karthik Kumar Chauhan, Divya Singh, Shweta Sinha, Nivedita Singh, Jang Bahadur Prakasha, T. L. Pal, Dharam Sivasamy, M. Jain, Neelu Singh, G. P. Singh, P. K. Front Plant Sci Plant Science There is a significant yield reduction in the wheat crop as a result of different biotic and abiotic stresses, and changing climate, among them moisture deficit stress and leaf rust are the major ones affecting wheat worldwide. HD3086 is a high-yielding wheat variety that has been released for commercial cultivation under timely sown irrigated conditions in the Indo-Gangetic plains of India. Variety HD3086 provides a good, stable yield, and it is the choice of millions of farmers in India. It becomes susceptible to the most prevalent pathotypes 77-5 and 77-9 of Puccinia triticina (causing leaf rust) in the production environment and its potential yield cannot be realized under moisture deficit stress. The present study demonstrates the use of a marker-assisted back cross breeding approach to the successful transfer of leaf rust resistance gene Lr24 and QTLs linked to moisture deficit stress tolerance in the background of HD3086. The genotype HI1500 was used as a donor parent that possesses leaf rust-resistant gene Lr24, which confers resistance against the major pathotypes found in the production environment. It possesses inbuilt tolerance under abiotic stresses with superior quality traits. Foreground selection for gene Lr24 and moisture deficit stress tolerance QTLs linked to Canopy temperature (CT), Normal Differential Vegetation Index (NDVI) and Thousand Kernel Weight (TKW) in different generations of the backcrossing and selection. In BC2F2, foreground selection was carried out to identify homozygous lines based on the linked markers and were advanced following pedigree based phenotypic selection. The selected lines were evaluated against P. triticina pathotypes 77-5 and 77-9 under controlled conditions. Recurrent parent recovery of the selected lines ranged from 78-94%. The identified lines were evaluated for their tolerance to moisture stress under field conditions and their resistance to rust under artificial epiphytotic conditions for two years. In BC2F5 generation, eight positive lines for marker alleles were selected which showed resistance to leaf rust and recorded an improvement in component traits of moisture deficit stress tolerance such as CT, NDVI, TKW and yield compared to the recurrent parent HD3086. The derived line is named HD3471 and is nominated for national trials for testing and further release for commercial cultivation. Frontiers Media S.A. 2022-10-24 /pmc/articles/PMC9638138/ /pubmed/36352858 http://dx.doi.org/10.3389/fpls.2022.1035016 Text en Copyright © 2022 Sunilkumar, Krishna, Devate, Manjunath, Chauhan, Singh, Sinha, Singh, Prakasha, Pal, Sivasamy, Jain, Singh and Singh https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Sunilkumar, V.P.
Krishna, Hari
Devate, Narayana Bhat
Manjunath, Karthik Kumar
Chauhan, Divya
Singh, Shweta
Sinha, Nivedita
Singh, Jang Bahadur
Prakasha, T. L.
Pal, Dharam
Sivasamy, M.
Jain, Neelu
Singh, G. P.
Singh, P. K.
Marker assisted improvement for leaf rust and moisture deficit stress tolerance in wheat variety HD3086
title Marker assisted improvement for leaf rust and moisture deficit stress tolerance in wheat variety HD3086
title_full Marker assisted improvement for leaf rust and moisture deficit stress tolerance in wheat variety HD3086
title_fullStr Marker assisted improvement for leaf rust and moisture deficit stress tolerance in wheat variety HD3086
title_full_unstemmed Marker assisted improvement for leaf rust and moisture deficit stress tolerance in wheat variety HD3086
title_short Marker assisted improvement for leaf rust and moisture deficit stress tolerance in wheat variety HD3086
title_sort marker assisted improvement for leaf rust and moisture deficit stress tolerance in wheat variety hd3086
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9638138/
https://www.ncbi.nlm.nih.gov/pubmed/36352858
http://dx.doi.org/10.3389/fpls.2022.1035016
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