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Hedgehog signaling can enhance glycolytic ATP production in the Drosophila wing disc
Adenosine triphosphate (ATP) production and utilization is critically important for animal development. How these processes are regulated in space and time during tissue growth remains largely unclear. We used a FRET‐based sensor to dynamically monitor ATP levels across a growing tissue, using the D...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9638854/ https://www.ncbi.nlm.nih.gov/pubmed/36134875 http://dx.doi.org/10.15252/embr.202154025 |
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author | Nellas, Ioannis Iyer, K Venkatesan Iglesias‐Artola, Juan M Pippel, Martin Nadler, André Eaton, Suzanne Dye, Natalie A |
author_facet | Nellas, Ioannis Iyer, K Venkatesan Iglesias‐Artola, Juan M Pippel, Martin Nadler, André Eaton, Suzanne Dye, Natalie A |
author_sort | Nellas, Ioannis |
collection | PubMed |
description | Adenosine triphosphate (ATP) production and utilization is critically important for animal development. How these processes are regulated in space and time during tissue growth remains largely unclear. We used a FRET‐based sensor to dynamically monitor ATP levels across a growing tissue, using the Drosophila larval wing disc. Although steady‐state levels of ATP are spatially uniform across the wing pouch, inhibiting oxidative phosphorylation reveals spatial differences in metabolic behavior, whereby signaling centers at compartment boundaries produce more ATP from glycolysis than the rest of the tissue. Genetic perturbations indicate that the conserved Hedgehog signaling pathway can enhance ATP production by glycolysis. Collectively, our work suggests the existence of a homeostatic feedback loop between Hh signaling and glycolysis, advancing our understanding of the connection between conserved developmental patterning genes and ATP production during animal tissue development. |
format | Online Article Text |
id | pubmed-9638854 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96388542022-11-14 Hedgehog signaling can enhance glycolytic ATP production in the Drosophila wing disc Nellas, Ioannis Iyer, K Venkatesan Iglesias‐Artola, Juan M Pippel, Martin Nadler, André Eaton, Suzanne Dye, Natalie A EMBO Rep Reports Adenosine triphosphate (ATP) production and utilization is critically important for animal development. How these processes are regulated in space and time during tissue growth remains largely unclear. We used a FRET‐based sensor to dynamically monitor ATP levels across a growing tissue, using the Drosophila larval wing disc. Although steady‐state levels of ATP are spatially uniform across the wing pouch, inhibiting oxidative phosphorylation reveals spatial differences in metabolic behavior, whereby signaling centers at compartment boundaries produce more ATP from glycolysis than the rest of the tissue. Genetic perturbations indicate that the conserved Hedgehog signaling pathway can enhance ATP production by glycolysis. Collectively, our work suggests the existence of a homeostatic feedback loop between Hh signaling and glycolysis, advancing our understanding of the connection between conserved developmental patterning genes and ATP production during animal tissue development. John Wiley and Sons Inc. 2022-09-22 /pmc/articles/PMC9638854/ /pubmed/36134875 http://dx.doi.org/10.15252/embr.202154025 Text en © 2022 The Authors. Published under the terms of the CC BY 4.0 license. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Reports Nellas, Ioannis Iyer, K Venkatesan Iglesias‐Artola, Juan M Pippel, Martin Nadler, André Eaton, Suzanne Dye, Natalie A Hedgehog signaling can enhance glycolytic ATP production in the Drosophila wing disc |
title | Hedgehog signaling can enhance glycolytic ATP production in the Drosophila wing disc |
title_full | Hedgehog signaling can enhance glycolytic ATP production in the Drosophila wing disc |
title_fullStr | Hedgehog signaling can enhance glycolytic ATP production in the Drosophila wing disc |
title_full_unstemmed | Hedgehog signaling can enhance glycolytic ATP production in the Drosophila wing disc |
title_short | Hedgehog signaling can enhance glycolytic ATP production in the Drosophila wing disc |
title_sort | hedgehog signaling can enhance glycolytic atp production in the drosophila wing disc |
topic | Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9638854/ https://www.ncbi.nlm.nih.gov/pubmed/36134875 http://dx.doi.org/10.15252/embr.202154025 |
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