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Gut microbiome-depleting antibiotic regimens are not tolerated by all mouse strains: learn from (our) bitter experience

Why the gut microbiome is critical for the success of checkpoint inhibitor cancer therapy is a question that remains unanswered, but progress has slowed. We argue that this lack of advancement is due to an unappreciated biological detail. Here, we show that the antibiotic cocktail used in seminal pu...

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Autores principales: Almonte, Andrew A, Cavic, George, Neeman, Teresa, Enders, Anselm, Fahrer, Aude M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9639134/
https://www.ncbi.nlm.nih.gov/pubmed/36323432
http://dx.doi.org/10.1136/jitc-2022-005575
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author Almonte, Andrew A
Cavic, George
Neeman, Teresa
Enders, Anselm
Fahrer, Aude M
author_facet Almonte, Andrew A
Cavic, George
Neeman, Teresa
Enders, Anselm
Fahrer, Aude M
author_sort Almonte, Andrew A
collection PubMed
description Why the gut microbiome is critical for the success of checkpoint inhibitor cancer therapy is a question that remains unanswered, but progress has slowed. We argue that this lack of advancement is due to an unappreciated biological detail. Here, we show that the antibiotic cocktail used in seminal publications—all of which have used the C57BL/6 mouse strain—are bitter and not tolerated by other common mouse strains (ie, BALB/c and DBA/2). We write to alert readers of this important biological limitation that must be considered when planning cancer experiments investigating the gut microbiota, to prevent the unnecessary dehydration of experimental animals, and to save our colleagues valuable experimental time and resources.
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spelling pubmed-96391342022-11-08 Gut microbiome-depleting antibiotic regimens are not tolerated by all mouse strains: learn from (our) bitter experience Almonte, Andrew A Cavic, George Neeman, Teresa Enders, Anselm Fahrer, Aude M J Immunother Cancer Commentary Why the gut microbiome is critical for the success of checkpoint inhibitor cancer therapy is a question that remains unanswered, but progress has slowed. We argue that this lack of advancement is due to an unappreciated biological detail. Here, we show that the antibiotic cocktail used in seminal publications—all of which have used the C57BL/6 mouse strain—are bitter and not tolerated by other common mouse strains (ie, BALB/c and DBA/2). We write to alert readers of this important biological limitation that must be considered when planning cancer experiments investigating the gut microbiota, to prevent the unnecessary dehydration of experimental animals, and to save our colleagues valuable experimental time and resources. BMJ Publishing Group 2022-11-02 /pmc/articles/PMC9639134/ /pubmed/36323432 http://dx.doi.org/10.1136/jitc-2022-005575 Text en © Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) .
spellingShingle Commentary
Almonte, Andrew A
Cavic, George
Neeman, Teresa
Enders, Anselm
Fahrer, Aude M
Gut microbiome-depleting antibiotic regimens are not tolerated by all mouse strains: learn from (our) bitter experience
title Gut microbiome-depleting antibiotic regimens are not tolerated by all mouse strains: learn from (our) bitter experience
title_full Gut microbiome-depleting antibiotic regimens are not tolerated by all mouse strains: learn from (our) bitter experience
title_fullStr Gut microbiome-depleting antibiotic regimens are not tolerated by all mouse strains: learn from (our) bitter experience
title_full_unstemmed Gut microbiome-depleting antibiotic regimens are not tolerated by all mouse strains: learn from (our) bitter experience
title_short Gut microbiome-depleting antibiotic regimens are not tolerated by all mouse strains: learn from (our) bitter experience
title_sort gut microbiome-depleting antibiotic regimens are not tolerated by all mouse strains: learn from (our) bitter experience
topic Commentary
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9639134/
https://www.ncbi.nlm.nih.gov/pubmed/36323432
http://dx.doi.org/10.1136/jitc-2022-005575
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