Cargando…
Uncover diagnostic immunity/hypoxia/ferroptosis/epithelial mesenchymal transformation-related CCR5, CD86, CD8A, ITGAM, and PTPRC in kidney transplantation patients with allograft rejection
The aim of this study was to identify predictive immunity/hypoxia/ferroptosis/epithelial mesenchymal transformation (EMT)-related biomarkers, pathways and new drugs in allograft rejection in kidney transplant patients. First, gene expression data were downloaded followed by identification of differe...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9639483/ https://www.ncbi.nlm.nih.gov/pubmed/36330810 http://dx.doi.org/10.1080/0886022X.2022.2141648 |
_version_ | 1784825651034849280 |
---|---|
author | He, Long Wang, Boqian Wang, Xueyi Liu, Yuewen Song, Xing Zhang, Yijian Li, Xin Yang, Hongwei |
author_facet | He, Long Wang, Boqian Wang, Xueyi Liu, Yuewen Song, Xing Zhang, Yijian Li, Xin Yang, Hongwei |
author_sort | He, Long |
collection | PubMed |
description | The aim of this study was to identify predictive immunity/hypoxia/ferroptosis/epithelial mesenchymal transformation (EMT)-related biomarkers, pathways and new drugs in allograft rejection in kidney transplant patients. First, gene expression data were downloaded followed by identification of differentially expressed genes (DEGs), weighted gene co-expression network analysis (WGCNA) and protein–protein interaction (PPI) analysis. Second, diagnostic model was construction based on key genes, followed by correlation analysis between immune/hypoxia/ferroptosis/EMT and key diagnostic genes. Finally, drug prediction of diagnostic key genes was carried out. Five diagnostic genes were further identified, including CCR5, CD86, CD8A, ITGAM, and PTPRC, which were positively correlated with allograft rejection after the kidney transplant. Highly infiltrated immune cells, highly expression of hypoxia-related genes and activated status of EMT were significantly positively correlated with five diagnostic genes. Interestingly, suppressors of ferroptosis (SOFs) and drivers of ferroptosis (DOFs) showed a complex regulatory relationship between ferroptosis and five diagnostic genes. CD86, CCR5, and ITGAM were respectively drug target of ABATACEPT, MARAVIROC, and CLARITHROMYCIN. PTPRC was drug target of both PREDNISONE and EPOETIN BETA. In conclusion, the study could be useful in understanding changes in the microenvironment within transplantation, which may promote or sustain the development of allograft rejection after kidney transplantation. |
format | Online Article Text |
id | pubmed-9639483 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-96394832022-11-08 Uncover diagnostic immunity/hypoxia/ferroptosis/epithelial mesenchymal transformation-related CCR5, CD86, CD8A, ITGAM, and PTPRC in kidney transplantation patients with allograft rejection He, Long Wang, Boqian Wang, Xueyi Liu, Yuewen Song, Xing Zhang, Yijian Li, Xin Yang, Hongwei Ren Fail Clinical Study The aim of this study was to identify predictive immunity/hypoxia/ferroptosis/epithelial mesenchymal transformation (EMT)-related biomarkers, pathways and new drugs in allograft rejection in kidney transplant patients. First, gene expression data were downloaded followed by identification of differentially expressed genes (DEGs), weighted gene co-expression network analysis (WGCNA) and protein–protein interaction (PPI) analysis. Second, diagnostic model was construction based on key genes, followed by correlation analysis between immune/hypoxia/ferroptosis/EMT and key diagnostic genes. Finally, drug prediction of diagnostic key genes was carried out. Five diagnostic genes were further identified, including CCR5, CD86, CD8A, ITGAM, and PTPRC, which were positively correlated with allograft rejection after the kidney transplant. Highly infiltrated immune cells, highly expression of hypoxia-related genes and activated status of EMT were significantly positively correlated with five diagnostic genes. Interestingly, suppressors of ferroptosis (SOFs) and drivers of ferroptosis (DOFs) showed a complex regulatory relationship between ferroptosis and five diagnostic genes. CD86, CCR5, and ITGAM were respectively drug target of ABATACEPT, MARAVIROC, and CLARITHROMYCIN. PTPRC was drug target of both PREDNISONE and EPOETIN BETA. In conclusion, the study could be useful in understanding changes in the microenvironment within transplantation, which may promote or sustain the development of allograft rejection after kidney transplantation. Taylor & Francis 2022-11-04 /pmc/articles/PMC9639483/ /pubmed/36330810 http://dx.doi.org/10.1080/0886022X.2022.2141648 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Clinical Study He, Long Wang, Boqian Wang, Xueyi Liu, Yuewen Song, Xing Zhang, Yijian Li, Xin Yang, Hongwei Uncover diagnostic immunity/hypoxia/ferroptosis/epithelial mesenchymal transformation-related CCR5, CD86, CD8A, ITGAM, and PTPRC in kidney transplantation patients with allograft rejection |
title | Uncover diagnostic immunity/hypoxia/ferroptosis/epithelial mesenchymal transformation-related CCR5, CD86, CD8A, ITGAM, and PTPRC in kidney transplantation patients with allograft rejection |
title_full | Uncover diagnostic immunity/hypoxia/ferroptosis/epithelial mesenchymal transformation-related CCR5, CD86, CD8A, ITGAM, and PTPRC in kidney transplantation patients with allograft rejection |
title_fullStr | Uncover diagnostic immunity/hypoxia/ferroptosis/epithelial mesenchymal transformation-related CCR5, CD86, CD8A, ITGAM, and PTPRC in kidney transplantation patients with allograft rejection |
title_full_unstemmed | Uncover diagnostic immunity/hypoxia/ferroptosis/epithelial mesenchymal transformation-related CCR5, CD86, CD8A, ITGAM, and PTPRC in kidney transplantation patients with allograft rejection |
title_short | Uncover diagnostic immunity/hypoxia/ferroptosis/epithelial mesenchymal transformation-related CCR5, CD86, CD8A, ITGAM, and PTPRC in kidney transplantation patients with allograft rejection |
title_sort | uncover diagnostic immunity/hypoxia/ferroptosis/epithelial mesenchymal transformation-related ccr5, cd86, cd8a, itgam, and ptprc in kidney transplantation patients with allograft rejection |
topic | Clinical Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9639483/ https://www.ncbi.nlm.nih.gov/pubmed/36330810 http://dx.doi.org/10.1080/0886022X.2022.2141648 |
work_keys_str_mv | AT helong uncoverdiagnosticimmunityhypoxiaferroptosisepithelialmesenchymaltransformationrelatedccr5cd86cd8aitgamandptprcinkidneytransplantationpatientswithallograftrejection AT wangboqian uncoverdiagnosticimmunityhypoxiaferroptosisepithelialmesenchymaltransformationrelatedccr5cd86cd8aitgamandptprcinkidneytransplantationpatientswithallograftrejection AT wangxueyi uncoverdiagnosticimmunityhypoxiaferroptosisepithelialmesenchymaltransformationrelatedccr5cd86cd8aitgamandptprcinkidneytransplantationpatientswithallograftrejection AT liuyuewen uncoverdiagnosticimmunityhypoxiaferroptosisepithelialmesenchymaltransformationrelatedccr5cd86cd8aitgamandptprcinkidneytransplantationpatientswithallograftrejection AT songxing uncoverdiagnosticimmunityhypoxiaferroptosisepithelialmesenchymaltransformationrelatedccr5cd86cd8aitgamandptprcinkidneytransplantationpatientswithallograftrejection AT zhangyijian uncoverdiagnosticimmunityhypoxiaferroptosisepithelialmesenchymaltransformationrelatedccr5cd86cd8aitgamandptprcinkidneytransplantationpatientswithallograftrejection AT lixin uncoverdiagnosticimmunityhypoxiaferroptosisepithelialmesenchymaltransformationrelatedccr5cd86cd8aitgamandptprcinkidneytransplantationpatientswithallograftrejection AT yanghongwei uncoverdiagnosticimmunityhypoxiaferroptosisepithelialmesenchymaltransformationrelatedccr5cd86cd8aitgamandptprcinkidneytransplantationpatientswithallograftrejection |