A mouse pancreatic organoid model to compare PD-L1 blocking antibodies

Immune checkpoint inhibitors (ICIs) have changed the therapeutic landscape for cancer patients, but diabetes, a rare, severe immune-related endocrinopathy, is linked to ICI therapy. It is unclear whether glycosylation of ICIs may play a role in the development of this adverse event and how the physi...

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Autores principales: Li, Guangyuan, Ghosh, Susmita, Park, JuMe, Shin, Hyunsu, Garige, Mamatha, Reaman, Gregory, Sourbier, Carole
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9639566/
https://www.ncbi.nlm.nih.gov/pubmed/36334035
http://dx.doi.org/10.1080/19420862.2022.2139886
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author Li, Guangyuan
Ghosh, Susmita
Park, JuMe
Shin, Hyunsu
Garige, Mamatha
Reaman, Gregory
Sourbier, Carole
author_facet Li, Guangyuan
Ghosh, Susmita
Park, JuMe
Shin, Hyunsu
Garige, Mamatha
Reaman, Gregory
Sourbier, Carole
author_sort Li, Guangyuan
collection PubMed
description Immune checkpoint inhibitors (ICIs) have changed the therapeutic landscape for cancer patients, but diabetes, a rare, severe immune-related endocrinopathy, is linked to ICI therapy. It is unclear whether glycosylation of ICIs may play a role in the development of this adverse event and how the physiological effects of different ICIs on pancreatic cells should be evaluated. We used a mouse pancreatic organoid model to compare three PD-L1 blocking antibodies in the presence or absence of IFNγ using a metabolic bioanalyzer. Modulation of ICI glycosylation altered its metabolic effects on mouse pancreatic organoids, suggesting that this model could be used to monitor and compare ICIs and to study the mechanisms underlying the development of IC-mediated diabetes.
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spelling pubmed-96395662023-02-07 A mouse pancreatic organoid model to compare PD-L1 blocking antibodies Li, Guangyuan Ghosh, Susmita Park, JuMe Shin, Hyunsu Garige, Mamatha Reaman, Gregory Sourbier, Carole MAbs Rapid Communication Immune checkpoint inhibitors (ICIs) have changed the therapeutic landscape for cancer patients, but diabetes, a rare, severe immune-related endocrinopathy, is linked to ICI therapy. It is unclear whether glycosylation of ICIs may play a role in the development of this adverse event and how the physiological effects of different ICIs on pancreatic cells should be evaluated. We used a mouse pancreatic organoid model to compare three PD-L1 blocking antibodies in the presence or absence of IFNγ using a metabolic bioanalyzer. Modulation of ICI glycosylation altered its metabolic effects on mouse pancreatic organoids, suggesting that this model could be used to monitor and compare ICIs and to study the mechanisms underlying the development of IC-mediated diabetes. Taylor & Francis 2022-11-05 /pmc/articles/PMC9639566/ /pubmed/36334035 http://dx.doi.org/10.1080/19420862.2022.2139886 Text en This work was authored as part of the Contributor’s official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 USC 105, no copyright protection is available for such works under US Law. https://creativecommons.org/publicdomain/mark/1.0/This is an Open Access article that has been identified as being free of known restrictions under copyright law, including all related and neighboring rights (https://creativecommons.org/publicdomain/mark/1.0/). You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission.
spellingShingle Rapid Communication
Li, Guangyuan
Ghosh, Susmita
Park, JuMe
Shin, Hyunsu
Garige, Mamatha
Reaman, Gregory
Sourbier, Carole
A mouse pancreatic organoid model to compare PD-L1 blocking antibodies
title A mouse pancreatic organoid model to compare PD-L1 blocking antibodies
title_full A mouse pancreatic organoid model to compare PD-L1 blocking antibodies
title_fullStr A mouse pancreatic organoid model to compare PD-L1 blocking antibodies
title_full_unstemmed A mouse pancreatic organoid model to compare PD-L1 blocking antibodies
title_short A mouse pancreatic organoid model to compare PD-L1 blocking antibodies
title_sort mouse pancreatic organoid model to compare pd-l1 blocking antibodies
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9639566/
https://www.ncbi.nlm.nih.gov/pubmed/36334035
http://dx.doi.org/10.1080/19420862.2022.2139886
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