Cargando…

Alterations in hepatic mitotic and cell cycle transcriptional networks during the metabolic switch in broiler chicks

During embryonic life, chicks mainly derive energy from hepatic oxidation of yolk lipids. After hatch, chicks must rely on carbohydrate-rich feed to obtain energy. This requires an abrupt and intensive switch of metabolic processes, particularly in the liver. We recently identified a number of trans...

Descripción completa

Detalles Bibliográficos
Autores principales: Hicks, Julie A., Pike, Brandon E., Liu, Hsiao-Ching
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9639855/
https://www.ncbi.nlm.nih.gov/pubmed/36353371
http://dx.doi.org/10.3389/fphys.2022.1020870
_version_ 1784825727689949184
author Hicks, Julie A.
Pike, Brandon E.
Liu, Hsiao-Ching
author_facet Hicks, Julie A.
Pike, Brandon E.
Liu, Hsiao-Ching
author_sort Hicks, Julie A.
collection PubMed
description During embryonic life, chicks mainly derive energy from hepatic oxidation of yolk lipids. After hatch, chicks must rely on carbohydrate-rich feed to obtain energy. This requires an abrupt and intensive switch of metabolic processes, particularly in the liver. We recently identified a number of transcriptional and post-transcriptional regulatory networks that work concordantly to tune metabolic processes during the metabolic switch. Here, we used delayed feeding post-hatch (48 h) to impede the metabolic switch in broilers. We used RNA-seq to identify hepatic transcriptome differences between late stage embryos (E18) and two-day-old chicks (D2), which were either fed-from-hatch (FED) or not fed (DLY). Between FED and E18, 2,430 genes were differentially expressed (fold-change≥ 2; FDR p-value 0.05), of these 1,237 were downregulated in FED birds and 1,193 were upregulated. Between DLY and E18, 1979 genes were differentially expressed, of these 1,043 were downregulated and 936 were upregulated in DLY birds. Between DLY and FED, 880 genes were differentially expressed, of these 543 were downregulated and 337 were upregulated in DLY birds. We found that in addition to disturbances in a number of metabolic pathways, unfed chicks had a widespread suppression of gene networks associated with cell proliferation, cell cycle progression and mitosis. Expression patterns suggest that hepatocytes of delayed-fed birds have abnormal mitosis and increased polyploidization. This suggests that post-hatch feed consumption maintains the rate and integrity of liver growth immediately, which in turn, likely helps facilitate the appropriate programming of hepatic metabolic networks.
format Online
Article
Text
id pubmed-9639855
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-96398552022-11-08 Alterations in hepatic mitotic and cell cycle transcriptional networks during the metabolic switch in broiler chicks Hicks, Julie A. Pike, Brandon E. Liu, Hsiao-Ching Front Physiol Physiology During embryonic life, chicks mainly derive energy from hepatic oxidation of yolk lipids. After hatch, chicks must rely on carbohydrate-rich feed to obtain energy. This requires an abrupt and intensive switch of metabolic processes, particularly in the liver. We recently identified a number of transcriptional and post-transcriptional regulatory networks that work concordantly to tune metabolic processes during the metabolic switch. Here, we used delayed feeding post-hatch (48 h) to impede the metabolic switch in broilers. We used RNA-seq to identify hepatic transcriptome differences between late stage embryos (E18) and two-day-old chicks (D2), which were either fed-from-hatch (FED) or not fed (DLY). Between FED and E18, 2,430 genes were differentially expressed (fold-change≥ 2; FDR p-value 0.05), of these 1,237 were downregulated in FED birds and 1,193 were upregulated. Between DLY and E18, 1979 genes were differentially expressed, of these 1,043 were downregulated and 936 were upregulated in DLY birds. Between DLY and FED, 880 genes were differentially expressed, of these 543 were downregulated and 337 were upregulated in DLY birds. We found that in addition to disturbances in a number of metabolic pathways, unfed chicks had a widespread suppression of gene networks associated with cell proliferation, cell cycle progression and mitosis. Expression patterns suggest that hepatocytes of delayed-fed birds have abnormal mitosis and increased polyploidization. This suggests that post-hatch feed consumption maintains the rate and integrity of liver growth immediately, which in turn, likely helps facilitate the appropriate programming of hepatic metabolic networks. Frontiers Media S.A. 2022-10-24 /pmc/articles/PMC9639855/ /pubmed/36353371 http://dx.doi.org/10.3389/fphys.2022.1020870 Text en Copyright © 2022 Hicks, Pike and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Hicks, Julie A.
Pike, Brandon E.
Liu, Hsiao-Ching
Alterations in hepatic mitotic and cell cycle transcriptional networks during the metabolic switch in broiler chicks
title Alterations in hepatic mitotic and cell cycle transcriptional networks during the metabolic switch in broiler chicks
title_full Alterations in hepatic mitotic and cell cycle transcriptional networks during the metabolic switch in broiler chicks
title_fullStr Alterations in hepatic mitotic and cell cycle transcriptional networks during the metabolic switch in broiler chicks
title_full_unstemmed Alterations in hepatic mitotic and cell cycle transcriptional networks during the metabolic switch in broiler chicks
title_short Alterations in hepatic mitotic and cell cycle transcriptional networks during the metabolic switch in broiler chicks
title_sort alterations in hepatic mitotic and cell cycle transcriptional networks during the metabolic switch in broiler chicks
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9639855/
https://www.ncbi.nlm.nih.gov/pubmed/36353371
http://dx.doi.org/10.3389/fphys.2022.1020870
work_keys_str_mv AT hicksjuliea alterationsinhepaticmitoticandcellcycletranscriptionalnetworksduringthemetabolicswitchinbroilerchicks
AT pikebrandone alterationsinhepaticmitoticandcellcycletranscriptionalnetworksduringthemetabolicswitchinbroilerchicks
AT liuhsiaoching alterationsinhepaticmitoticandcellcycletranscriptionalnetworksduringthemetabolicswitchinbroilerchicks