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Identification of phosphorylation site on PARP1 mediating its cytosolic translocation in virus-infected HeLa cells

Poly (ADP-ribose) polymerase 1 (PARP1) localization is controlled by its phosphorylation state. Here, we describe a protocol to monitor PARP1 subcellular localization in HSV-1-infected HeLa cells using immunofluorescence microscopy and cytoplasmic/nuclear fractionation. We detail steps to identify p...

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Detalles Bibliográficos
Autores principales:	Wang, Fei, Ma, Ming Tong, Xu, Junfang, Liu, Haipeng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9640338/ https://www.ncbi.nlm.nih.gov/pubmed/36386864 http://dx.doi.org/10.1016/j.xpro.2022.101808

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