Using a zebrafish model to understand adherent-invasive Escherichia coli infection

A zebrafish model was developed to study AIEC colonization, invasion, and inflammation. This model can also be used to study the beneficial effects of a probiotic on AIEC infection of adult zebrafish. Bacteria are grown in vitro and then fish are infected with AIEC by immersion. Subsequently, coloni...

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Autores principales: Nag, Dhrubajyoti, Farr, Dustin, Raychaudhuri, Saumya, Withey, Jeffrey H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9640341/
https://www.ncbi.nlm.nih.gov/pubmed/36386878
http://dx.doi.org/10.1016/j.xpro.2022.101810
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author Nag, Dhrubajyoti
Farr, Dustin
Raychaudhuri, Saumya
Withey, Jeffrey H.
author_facet Nag, Dhrubajyoti
Farr, Dustin
Raychaudhuri, Saumya
Withey, Jeffrey H.
author_sort Nag, Dhrubajyoti
collection PubMed
description A zebrafish model was developed to study AIEC colonization, invasion, and inflammation. This model can also be used to study the beneficial effects of a probiotic on AIEC infection of adult zebrafish. Bacteria are grown in vitro and then fish are infected with AIEC by immersion. Subsequently, colonization and inflammation can be assessed. Exposing fish to probiotic at different time points relative to AIEC can determine beneficial effects of probiotics as prophylactics or therapeutics against AIEC. For complete details on the use and execution of this protocol, please refer to Nag et al. (2022).
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spelling pubmed-96403412022-11-15 Using a zebrafish model to understand adherent-invasive Escherichia coli infection Nag, Dhrubajyoti Farr, Dustin Raychaudhuri, Saumya Withey, Jeffrey H. STAR Protoc Protocol A zebrafish model was developed to study AIEC colonization, invasion, and inflammation. This model can also be used to study the beneficial effects of a probiotic on AIEC infection of adult zebrafish. Bacteria are grown in vitro and then fish are infected with AIEC by immersion. Subsequently, colonization and inflammation can be assessed. Exposing fish to probiotic at different time points relative to AIEC can determine beneficial effects of probiotics as prophylactics or therapeutics against AIEC. For complete details on the use and execution of this protocol, please refer to Nag et al. (2022). Elsevier 2022-11-02 /pmc/articles/PMC9640341/ /pubmed/36386878 http://dx.doi.org/10.1016/j.xpro.2022.101810 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Nag, Dhrubajyoti
Farr, Dustin
Raychaudhuri, Saumya
Withey, Jeffrey H.
Using a zebrafish model to understand adherent-invasive Escherichia coli infection
title Using a zebrafish model to understand adherent-invasive Escherichia coli infection
title_full Using a zebrafish model to understand adherent-invasive Escherichia coli infection
title_fullStr Using a zebrafish model to understand adherent-invasive Escherichia coli infection
title_full_unstemmed Using a zebrafish model to understand adherent-invasive Escherichia coli infection
title_short Using a zebrafish model to understand adherent-invasive Escherichia coli infection
title_sort using a zebrafish model to understand adherent-invasive escherichia coli infection
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9640341/
https://www.ncbi.nlm.nih.gov/pubmed/36386878
http://dx.doi.org/10.1016/j.xpro.2022.101810
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