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Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae)

The current work is aimed at isolating and identifying new Entomopathogenic bacterium (EPB) strains associated with Steinernema feltiae and assessing the EPB’s biocontrol potential on Aphis punicae and Aphis illinoisensis adults in the laboratory. From S. feltiae, five bacterial isolates were isolat...

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Autores principales: Baazeem, Alaa, Alotaibi, Saqer S., Khalaf, Luaay Kahtan, Kumar, Uttam, Zaynab, Madiha, Alharthi, Sarah, Darwish, Hadeer, Alghamdi, Akram, Jat, Suresh Kumar, Al-Barty, Amal, Albogami, Bander, Noureldeen, Ahmed, Ravindran, Balasubramani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9640465/
https://www.ncbi.nlm.nih.gov/pubmed/36386662
http://dx.doi.org/10.3389/fmicb.2022.961349
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author Baazeem, Alaa
Alotaibi, Saqer S.
Khalaf, Luaay Kahtan
Kumar, Uttam
Zaynab, Madiha
Alharthi, Sarah
Darwish, Hadeer
Alghamdi, Akram
Jat, Suresh Kumar
Al-Barty, Amal
Albogami, Bander
Noureldeen, Ahmed
Ravindran, Balasubramani
author_facet Baazeem, Alaa
Alotaibi, Saqer S.
Khalaf, Luaay Kahtan
Kumar, Uttam
Zaynab, Madiha
Alharthi, Sarah
Darwish, Hadeer
Alghamdi, Akram
Jat, Suresh Kumar
Al-Barty, Amal
Albogami, Bander
Noureldeen, Ahmed
Ravindran, Balasubramani
author_sort Baazeem, Alaa
collection PubMed
description The current work is aimed at isolating and identifying new Entomopathogenic bacterium (EPB) strains associated with Steinernema feltiae and assessing the EPB’s biocontrol potential on Aphis punicae and Aphis illinoisensis adults in the laboratory. From S. feltiae, five bacterial isolates were isolated and molecularly characterized. Lysinibacillus xylanilyticus strain TU-2, Lysinibacillus xylanilyticus strain BN-13, Serratia liquefaciens strain TU-6, Stenotrophomonas tumulicola strain T5916-2-1b, and Pseudochrobactrum saccharolyticum strain CCUG are the strains. Pathogenicity tests demonstrated that bacterial cells were more toxic against the two aphid species than bacterial cell-free supernatants. S. tumulicola strain T5916-2-1b cells and filtrate were reported to have the strongest potential to kill A. punicae and A. illinoisensis individuals within 6 h after treatment, with 100% mortality of both insects 24 and 48 h after treatment. Based on the results of the study, it looked like endogenous Steinernema-associated EPB could be used directly as a biocontrol agent for A. punicae and A. illinoisensis.
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spelling pubmed-96404652022-11-15 Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae) Baazeem, Alaa Alotaibi, Saqer S. Khalaf, Luaay Kahtan Kumar, Uttam Zaynab, Madiha Alharthi, Sarah Darwish, Hadeer Alghamdi, Akram Jat, Suresh Kumar Al-Barty, Amal Albogami, Bander Noureldeen, Ahmed Ravindran, Balasubramani Front Microbiol Microbiology The current work is aimed at isolating and identifying new Entomopathogenic bacterium (EPB) strains associated with Steinernema feltiae and assessing the EPB’s biocontrol potential on Aphis punicae and Aphis illinoisensis adults in the laboratory. From S. feltiae, five bacterial isolates were isolated and molecularly characterized. Lysinibacillus xylanilyticus strain TU-2, Lysinibacillus xylanilyticus strain BN-13, Serratia liquefaciens strain TU-6, Stenotrophomonas tumulicola strain T5916-2-1b, and Pseudochrobactrum saccharolyticum strain CCUG are the strains. Pathogenicity tests demonstrated that bacterial cells were more toxic against the two aphid species than bacterial cell-free supernatants. S. tumulicola strain T5916-2-1b cells and filtrate were reported to have the strongest potential to kill A. punicae and A. illinoisensis individuals within 6 h after treatment, with 100% mortality of both insects 24 and 48 h after treatment. Based on the results of the study, it looked like endogenous Steinernema-associated EPB could be used directly as a biocontrol agent for A. punicae and A. illinoisensis. Frontiers Media S.A. 2022-10-25 /pmc/articles/PMC9640465/ /pubmed/36386662 http://dx.doi.org/10.3389/fmicb.2022.961349 Text en Copyright © 2022 Baazeem, Alotaibi, Khalaf, Kumar, Zaynab, Alharthi, Darwish, Alghamdi, Jat, Al-Barty, Albogami, Noureldeen and Ravindran. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Baazeem, Alaa
Alotaibi, Saqer S.
Khalaf, Luaay Kahtan
Kumar, Uttam
Zaynab, Madiha
Alharthi, Sarah
Darwish, Hadeer
Alghamdi, Akram
Jat, Suresh Kumar
Al-Barty, Amal
Albogami, Bander
Noureldeen, Ahmed
Ravindran, Balasubramani
Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae)
title Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae)
title_full Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae)
title_fullStr Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae)
title_full_unstemmed Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae)
title_short Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae)
title_sort identification and environment-friendly biocontrol potential of five different bacteria against aphis punicae and aphis illinoisensis (hemiptera: aphididae)
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9640465/
https://www.ncbi.nlm.nih.gov/pubmed/36386662
http://dx.doi.org/10.3389/fmicb.2022.961349
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