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Molecular co-localization of multiple drugs in a nanoscopic delivery vehicle for potential synergistic remediation of multi-drug resistant bacteria

Anti-microbial resistant infection is predicted to be alarming in upcoming years. In the present study, we proposed co-localization of two model drugs viz., rifampicin and benzothiazole used in anti-tuberculosis and anti-fungal agents respectively in a nanoscopic cationic micelle (cetyl triethyl amm...

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Autores principales: Banerjee, Amrita, Mukherjee, Dipanjan, Bera, Arpan, Ghosh, Ria, Mondal, Susmita, Mukhopadhyay, Subhadipta, Das, Ranjan, Altass, Hatem M., Natto, Sameer. S. A., Moussa, Ziad, Ahmed, Saleh A., Chattopadhyay, Arpita, Pal, Samir Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9640573/
https://www.ncbi.nlm.nih.gov/pubmed/36344591
http://dx.doi.org/10.1038/s41598-022-22759-z
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author Banerjee, Amrita
Mukherjee, Dipanjan
Bera, Arpan
Ghosh, Ria
Mondal, Susmita
Mukhopadhyay, Subhadipta
Das, Ranjan
Altass, Hatem M.
Natto, Sameer. S. A.
Moussa, Ziad
Ahmed, Saleh A.
Chattopadhyay, Arpita
Pal, Samir Kumar
author_facet Banerjee, Amrita
Mukherjee, Dipanjan
Bera, Arpan
Ghosh, Ria
Mondal, Susmita
Mukhopadhyay, Subhadipta
Das, Ranjan
Altass, Hatem M.
Natto, Sameer. S. A.
Moussa, Ziad
Ahmed, Saleh A.
Chattopadhyay, Arpita
Pal, Samir Kumar
author_sort Banerjee, Amrita
collection PubMed
description Anti-microbial resistant infection is predicted to be alarming in upcoming years. In the present study, we proposed co-localization of two model drugs viz., rifampicin and benzothiazole used in anti-tuberculosis and anti-fungal agents respectively in a nanoscopic cationic micelle (cetyl triethyl ammonium bromide) with hydrodynamic diameter of 2.69 nm. Sterilization effect of the co-localized micellar formulation against a model multi-drug resistant bacterial strain viz., Methicillin resistant Staphylococcus aureus was also investigated. 99.88% decrease of bacterial growth in terms of colony forming unit was observed using the developed formulation. While Dynamic Light Scattering and Forsters Resonance Energy Transfer between benzothiazole and rifampicin show co-localization of the drugs in the nanoscopic micellar environment, analysis of time-resolved fluorescence decays by Infelta-Tachiya model and the probability distribution of the donor–acceptor distance fluctuations for 5 μM,10 μM and 15 μM acceptor concentrations confirm efficacy of the co-localization. Energy transfer efficiency and the donor acceptor distance are found to be 46% and 20.9 Å respectively. We have also used a detailed computational biology framework to rationalize the sterilization effect of our indigenous formulation. It has to be noted that the drugs used in our studies are not being used for their conventional indication. Rather the co-localization of the drugs in the micellar environment shows a completely different indication of their use in the remediation of multi-drug resistant bacteria revealing the re-purposing of the drugs for potential use in hospital-born multi-drug resistant bacterial infection.
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spelling pubmed-96405732022-11-15 Molecular co-localization of multiple drugs in a nanoscopic delivery vehicle for potential synergistic remediation of multi-drug resistant bacteria Banerjee, Amrita Mukherjee, Dipanjan Bera, Arpan Ghosh, Ria Mondal, Susmita Mukhopadhyay, Subhadipta Das, Ranjan Altass, Hatem M. Natto, Sameer. S. A. Moussa, Ziad Ahmed, Saleh A. Chattopadhyay, Arpita Pal, Samir Kumar Sci Rep Article Anti-microbial resistant infection is predicted to be alarming in upcoming years. In the present study, we proposed co-localization of two model drugs viz., rifampicin and benzothiazole used in anti-tuberculosis and anti-fungal agents respectively in a nanoscopic cationic micelle (cetyl triethyl ammonium bromide) with hydrodynamic diameter of 2.69 nm. Sterilization effect of the co-localized micellar formulation against a model multi-drug resistant bacterial strain viz., Methicillin resistant Staphylococcus aureus was also investigated. 99.88% decrease of bacterial growth in terms of colony forming unit was observed using the developed formulation. While Dynamic Light Scattering and Forsters Resonance Energy Transfer between benzothiazole and rifampicin show co-localization of the drugs in the nanoscopic micellar environment, analysis of time-resolved fluorescence decays by Infelta-Tachiya model and the probability distribution of the donor–acceptor distance fluctuations for 5 μM,10 μM and 15 μM acceptor concentrations confirm efficacy of the co-localization. Energy transfer efficiency and the donor acceptor distance are found to be 46% and 20.9 Å respectively. We have also used a detailed computational biology framework to rationalize the sterilization effect of our indigenous formulation. It has to be noted that the drugs used in our studies are not being used for their conventional indication. Rather the co-localization of the drugs in the micellar environment shows a completely different indication of their use in the remediation of multi-drug resistant bacteria revealing the re-purposing of the drugs for potential use in hospital-born multi-drug resistant bacterial infection. Nature Publishing Group UK 2022-11-07 /pmc/articles/PMC9640573/ /pubmed/36344591 http://dx.doi.org/10.1038/s41598-022-22759-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Banerjee, Amrita
Mukherjee, Dipanjan
Bera, Arpan
Ghosh, Ria
Mondal, Susmita
Mukhopadhyay, Subhadipta
Das, Ranjan
Altass, Hatem M.
Natto, Sameer. S. A.
Moussa, Ziad
Ahmed, Saleh A.
Chattopadhyay, Arpita
Pal, Samir Kumar
Molecular co-localization of multiple drugs in a nanoscopic delivery vehicle for potential synergistic remediation of multi-drug resistant bacteria
title Molecular co-localization of multiple drugs in a nanoscopic delivery vehicle for potential synergistic remediation of multi-drug resistant bacteria
title_full Molecular co-localization of multiple drugs in a nanoscopic delivery vehicle for potential synergistic remediation of multi-drug resistant bacteria
title_fullStr Molecular co-localization of multiple drugs in a nanoscopic delivery vehicle for potential synergistic remediation of multi-drug resistant bacteria
title_full_unstemmed Molecular co-localization of multiple drugs in a nanoscopic delivery vehicle for potential synergistic remediation of multi-drug resistant bacteria
title_short Molecular co-localization of multiple drugs in a nanoscopic delivery vehicle for potential synergistic remediation of multi-drug resistant bacteria
title_sort molecular co-localization of multiple drugs in a nanoscopic delivery vehicle for potential synergistic remediation of multi-drug resistant bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9640573/
https://www.ncbi.nlm.nih.gov/pubmed/36344591
http://dx.doi.org/10.1038/s41598-022-22759-z
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