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Functionalized graphene grids with various charges for single-particle cryo-EM
A major hurdle for single particle cryo-EM in structural determination lies in the specimen preparation impaired by the air-water interface (AWI) and preferential particle-orientation problems. In this work, we develop functionalized graphene grids with various charges via a dediazoniation reaction...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9640669/ https://www.ncbi.nlm.nih.gov/pubmed/36344519 http://dx.doi.org/10.1038/s41467-022-34579-w |
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author | Lu, Ye Liu, Nan Liu, Yongbo Zheng, Liming Yang, Junhao Wang, Jia Jia, Xia Zi, Qinru Peng, Hailin Rao, Yu Wang, Hong-Wei |
author_facet | Lu, Ye Liu, Nan Liu, Yongbo Zheng, Liming Yang, Junhao Wang, Jia Jia, Xia Zi, Qinru Peng, Hailin Rao, Yu Wang, Hong-Wei |
author_sort | Lu, Ye |
collection | PubMed |
description | A major hurdle for single particle cryo-EM in structural determination lies in the specimen preparation impaired by the air-water interface (AWI) and preferential particle-orientation problems. In this work, we develop functionalized graphene grids with various charges via a dediazoniation reaction for cryo-EM specimen preparation. The graphene grids are paraffin-assistant fabricated, which appear with less contaminations compared with those produced by polymer transfer method. By applying onto three different types of macromolecules, we demonstrate that the high-yield charged graphene grids bring macromolecules away from the AWI and enable adjustable particle-orientation distribution for more robust single particle cryo-EM structural determination. |
format | Online Article Text |
id | pubmed-9640669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-96406692022-11-15 Functionalized graphene grids with various charges for single-particle cryo-EM Lu, Ye Liu, Nan Liu, Yongbo Zheng, Liming Yang, Junhao Wang, Jia Jia, Xia Zi, Qinru Peng, Hailin Rao, Yu Wang, Hong-Wei Nat Commun Article A major hurdle for single particle cryo-EM in structural determination lies in the specimen preparation impaired by the air-water interface (AWI) and preferential particle-orientation problems. In this work, we develop functionalized graphene grids with various charges via a dediazoniation reaction for cryo-EM specimen preparation. The graphene grids are paraffin-assistant fabricated, which appear with less contaminations compared with those produced by polymer transfer method. By applying onto three different types of macromolecules, we demonstrate that the high-yield charged graphene grids bring macromolecules away from the AWI and enable adjustable particle-orientation distribution for more robust single particle cryo-EM structural determination. Nature Publishing Group UK 2022-11-07 /pmc/articles/PMC9640669/ /pubmed/36344519 http://dx.doi.org/10.1038/s41467-022-34579-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Lu, Ye Liu, Nan Liu, Yongbo Zheng, Liming Yang, Junhao Wang, Jia Jia, Xia Zi, Qinru Peng, Hailin Rao, Yu Wang, Hong-Wei Functionalized graphene grids with various charges for single-particle cryo-EM |
title | Functionalized graphene grids with various charges for single-particle cryo-EM |
title_full | Functionalized graphene grids with various charges for single-particle cryo-EM |
title_fullStr | Functionalized graphene grids with various charges for single-particle cryo-EM |
title_full_unstemmed | Functionalized graphene grids with various charges for single-particle cryo-EM |
title_short | Functionalized graphene grids with various charges for single-particle cryo-EM |
title_sort | functionalized graphene grids with various charges for single-particle cryo-em |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9640669/ https://www.ncbi.nlm.nih.gov/pubmed/36344519 http://dx.doi.org/10.1038/s41467-022-34579-w |
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