Cargando…
Comparative and pangenomic analysis of the genus Streptomyces
Streptomycetes are highly metabolically gifted bacteria with the abilities to produce bioproducts that have profound economic and societal importance. These bioproducts are produced by metabolic pathways including those for the biosynthesis of secondary metabolites and catabolism of plant biomass co...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9640686/ https://www.ncbi.nlm.nih.gov/pubmed/36344558 http://dx.doi.org/10.1038/s41598-022-21731-1 |
_version_ | 1784825913647562752 |
---|---|
author | Otani, Hiroshi Udwary, Daniel W. Mouncey, Nigel J. |
author_facet | Otani, Hiroshi Udwary, Daniel W. Mouncey, Nigel J. |
author_sort | Otani, Hiroshi |
collection | PubMed |
description | Streptomycetes are highly metabolically gifted bacteria with the abilities to produce bioproducts that have profound economic and societal importance. These bioproducts are produced by metabolic pathways including those for the biosynthesis of secondary metabolites and catabolism of plant biomass constituents. Advancements in genome sequencing technologies have revealed a wealth of untapped metabolic potential from Streptomyces genomes. Here, we report the largest Streptomyces pangenome generated by using 205 complete genomes. Metabolic potentials of the pangenome and individual genomes were analyzed, revealing degrees of conservation of individual metabolic pathways and strains potentially suitable for metabolic engineering. Of them, Streptomyces bingchenggensis was identified as a potent degrader of plant biomass. Polyketide, non-ribosomal peptide, and gamma-butyrolactone biosynthetic enzymes are primarily strain specific while ectoine and some terpene biosynthetic pathways are highly conserved. A large number of transcription factors associated with secondary metabolism are strain-specific while those controlling basic biological processes are highly conserved. Although the majority of genes involved in morphological development are highly conserved, there are strain-specific varieties which may contribute to fine tuning the timing of cellular differentiation. Overall, these results provide insights into the metabolic potential, regulation and physiology of streptomycetes, which will facilitate further exploitation of these important bacteria. |
format | Online Article Text |
id | pubmed-9640686 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-96406862022-11-15 Comparative and pangenomic analysis of the genus Streptomyces Otani, Hiroshi Udwary, Daniel W. Mouncey, Nigel J. Sci Rep Article Streptomycetes are highly metabolically gifted bacteria with the abilities to produce bioproducts that have profound economic and societal importance. These bioproducts are produced by metabolic pathways including those for the biosynthesis of secondary metabolites and catabolism of plant biomass constituents. Advancements in genome sequencing technologies have revealed a wealth of untapped metabolic potential from Streptomyces genomes. Here, we report the largest Streptomyces pangenome generated by using 205 complete genomes. Metabolic potentials of the pangenome and individual genomes were analyzed, revealing degrees of conservation of individual metabolic pathways and strains potentially suitable for metabolic engineering. Of them, Streptomyces bingchenggensis was identified as a potent degrader of plant biomass. Polyketide, non-ribosomal peptide, and gamma-butyrolactone biosynthetic enzymes are primarily strain specific while ectoine and some terpene biosynthetic pathways are highly conserved. A large number of transcription factors associated with secondary metabolism are strain-specific while those controlling basic biological processes are highly conserved. Although the majority of genes involved in morphological development are highly conserved, there are strain-specific varieties which may contribute to fine tuning the timing of cellular differentiation. Overall, these results provide insights into the metabolic potential, regulation and physiology of streptomycetes, which will facilitate further exploitation of these important bacteria. Nature Publishing Group UK 2022-11-07 /pmc/articles/PMC9640686/ /pubmed/36344558 http://dx.doi.org/10.1038/s41598-022-21731-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Otani, Hiroshi Udwary, Daniel W. Mouncey, Nigel J. Comparative and pangenomic analysis of the genus Streptomyces |
title | Comparative and pangenomic analysis of the genus Streptomyces |
title_full | Comparative and pangenomic analysis of the genus Streptomyces |
title_fullStr | Comparative and pangenomic analysis of the genus Streptomyces |
title_full_unstemmed | Comparative and pangenomic analysis of the genus Streptomyces |
title_short | Comparative and pangenomic analysis of the genus Streptomyces |
title_sort | comparative and pangenomic analysis of the genus streptomyces |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9640686/ https://www.ncbi.nlm.nih.gov/pubmed/36344558 http://dx.doi.org/10.1038/s41598-022-21731-1 |
work_keys_str_mv | AT otanihiroshi comparativeandpangenomicanalysisofthegenusstreptomyces AT udwarydanielw comparativeandpangenomicanalysisofthegenusstreptomyces AT mounceynigelj comparativeandpangenomicanalysisofthegenusstreptomyces |