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Single-cell mechanistic studies of radiation-mediated bystander effects

Ionizing radiation (IR) has been widely used in the diagnosis and treatment of clinical diseases, with radiation therapy (RT) being particularly rapid, but it can induce “bystander effects” that lead to biological responses in non-target cells after their neighboring cells have been irradiated. To h...

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Autores principales: Han, Xueqiong, Chen, Yixuan, Zhang, Nan, Huang, Chengyu, He, Guangyao, Li, Ting, Wei, Mengxin, Song, Qiong, Mo, Shaowen, Lv, Yufeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9640915/
https://www.ncbi.nlm.nih.gov/pubmed/36389749
http://dx.doi.org/10.3389/fimmu.2022.849341
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author Han, Xueqiong
Chen, Yixuan
Zhang, Nan
Huang, Chengyu
He, Guangyao
Li, Ting
Wei, Mengxin
Song, Qiong
Mo, Shaowen
Lv, Yufeng
author_facet Han, Xueqiong
Chen, Yixuan
Zhang, Nan
Huang, Chengyu
He, Guangyao
Li, Ting
Wei, Mengxin
Song, Qiong
Mo, Shaowen
Lv, Yufeng
author_sort Han, Xueqiong
collection PubMed
description Ionizing radiation (IR) has been widely used in the diagnosis and treatment of clinical diseases, with radiation therapy (RT) being particularly rapid, but it can induce “bystander effects” that lead to biological responses in non-target cells after their neighboring cells have been irradiated. To help clarify how radiotherapy induces these effects, To help clarify how radiotherapy induces these effects, we analyzed single-cell RNA sequencing data from irradiated intestinal tissues on day 1 (T1 state), day 3 (T3 state), day 7 (T7 state), and day 14 (T14 state) after irradiation, as well as from healthy intestinal tissues (T0 state), to reveal the cellular level, molecular level, and involvement of different time irradiated mouse intestinal tissues in biological signaling pathways. In addition, changes in immune cell subpopulations and myeloid cell subpopulations after different radiation times were further explored, and gene regulatory networks (GRNs) of these cell subpopulations were constructed. Cellular communication between radiation-specific immune cells was explored by cell-to-cell communication events. The results suggest that radiotherapy trigger changes in immune cell subsets, which then reprogram the immune ecosystem and mediate systemic bystander effects. These radiation-specific immune cells participate in a wide range of cell-to-cell communication events. In particular, radiation-specific CD8+T cells appear to be at the core of communication and appear to persist in the body after recovery from radiotherapy, with enrichment analysis showing that radiation-specific CD8+ T cells are associated with ferroptosis. Thus, radiation-specific CD8+ T cells may be involved in cellular ferroptosis-mediated adverse effects caused by RT.
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spelling pubmed-96409152022-11-15 Single-cell mechanistic studies of radiation-mediated bystander effects Han, Xueqiong Chen, Yixuan Zhang, Nan Huang, Chengyu He, Guangyao Li, Ting Wei, Mengxin Song, Qiong Mo, Shaowen Lv, Yufeng Front Immunol Immunology Ionizing radiation (IR) has been widely used in the diagnosis and treatment of clinical diseases, with radiation therapy (RT) being particularly rapid, but it can induce “bystander effects” that lead to biological responses in non-target cells after their neighboring cells have been irradiated. To help clarify how radiotherapy induces these effects, To help clarify how radiotherapy induces these effects, we analyzed single-cell RNA sequencing data from irradiated intestinal tissues on day 1 (T1 state), day 3 (T3 state), day 7 (T7 state), and day 14 (T14 state) after irradiation, as well as from healthy intestinal tissues (T0 state), to reveal the cellular level, molecular level, and involvement of different time irradiated mouse intestinal tissues in biological signaling pathways. In addition, changes in immune cell subpopulations and myeloid cell subpopulations after different radiation times were further explored, and gene regulatory networks (GRNs) of these cell subpopulations were constructed. Cellular communication between radiation-specific immune cells was explored by cell-to-cell communication events. The results suggest that radiotherapy trigger changes in immune cell subsets, which then reprogram the immune ecosystem and mediate systemic bystander effects. These radiation-specific immune cells participate in a wide range of cell-to-cell communication events. In particular, radiation-specific CD8+T cells appear to be at the core of communication and appear to persist in the body after recovery from radiotherapy, with enrichment analysis showing that radiation-specific CD8+ T cells are associated with ferroptosis. Thus, radiation-specific CD8+ T cells may be involved in cellular ferroptosis-mediated adverse effects caused by RT. Frontiers Media S.A. 2022-10-25 /pmc/articles/PMC9640915/ /pubmed/36389749 http://dx.doi.org/10.3389/fimmu.2022.849341 Text en Copyright © 2022 Han, Chen, Zhang, Huang, He, Li, Wei, Song, Mo and Lv https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Han, Xueqiong
Chen, Yixuan
Zhang, Nan
Huang, Chengyu
He, Guangyao
Li, Ting
Wei, Mengxin
Song, Qiong
Mo, Shaowen
Lv, Yufeng
Single-cell mechanistic studies of radiation-mediated bystander effects
title Single-cell mechanistic studies of radiation-mediated bystander effects
title_full Single-cell mechanistic studies of radiation-mediated bystander effects
title_fullStr Single-cell mechanistic studies of radiation-mediated bystander effects
title_full_unstemmed Single-cell mechanistic studies of radiation-mediated bystander effects
title_short Single-cell mechanistic studies of radiation-mediated bystander effects
title_sort single-cell mechanistic studies of radiation-mediated bystander effects
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9640915/
https://www.ncbi.nlm.nih.gov/pubmed/36389749
http://dx.doi.org/10.3389/fimmu.2022.849341
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