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Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata

Plasmodesmata (PD) pores connect neighbouring plant cells and enable direct transport across the cell wall. Understanding the molecular composition of these structures is essential to address their formation and later dynamic regulation. Here we provide a biochemical characterisation of the cell wal...

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Autores principales: Paterlini, A., Sechet, J., Immel, F., Grison, M. S., Pilard, S., Pelloux, J., Mouille, G., Bayer, E. M., Voxeur, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9640925/
https://www.ncbi.nlm.nih.gov/pubmed/36388604
http://dx.doi.org/10.3389/fpls.2022.1020506
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author Paterlini, A.
Sechet, J.
Immel, F.
Grison, M. S.
Pilard, S.
Pelloux, J.
Mouille, G.
Bayer, E. M.
Voxeur, A.
author_facet Paterlini, A.
Sechet, J.
Immel, F.
Grison, M. S.
Pilard, S.
Pelloux, J.
Mouille, G.
Bayer, E. M.
Voxeur, A.
author_sort Paterlini, A.
collection PubMed
description Plasmodesmata (PD) pores connect neighbouring plant cells and enable direct transport across the cell wall. Understanding the molecular composition of these structures is essential to address their formation and later dynamic regulation. Here we provide a biochemical characterisation of the cell wall co-purified with primary PD of Arabidopsis thaliana cell cultures. To achieve this result we combined subcellular fractionation, polysaccharide analyses and enzymatic fingerprinting approaches. Relative to the rest of the cell wall, specific patterns were observed in the PD fraction. Most xyloglucans, although possibly not abundant as a group, were fucosylated. Homogalacturonans displayed short methylated stretches while rhamnogalacturonan I species were remarkably abundant. Full rhamnogalacturonan II forms, highly methyl-acetylated, were also present. We additionally showed that these domains, compared to the broad wall, are less affected by wall modifying activities during a time interval of days. Overall, the protocol and the data presented here open new opportunities for the study of wall polysaccharides associated with PD.
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spelling pubmed-96409252022-11-15 Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata Paterlini, A. Sechet, J. Immel, F. Grison, M. S. Pilard, S. Pelloux, J. Mouille, G. Bayer, E. M. Voxeur, A. Front Plant Sci Plant Science Plasmodesmata (PD) pores connect neighbouring plant cells and enable direct transport across the cell wall. Understanding the molecular composition of these structures is essential to address their formation and later dynamic regulation. Here we provide a biochemical characterisation of the cell wall co-purified with primary PD of Arabidopsis thaliana cell cultures. To achieve this result we combined subcellular fractionation, polysaccharide analyses and enzymatic fingerprinting approaches. Relative to the rest of the cell wall, specific patterns were observed in the PD fraction. Most xyloglucans, although possibly not abundant as a group, were fucosylated. Homogalacturonans displayed short methylated stretches while rhamnogalacturonan I species were remarkably abundant. Full rhamnogalacturonan II forms, highly methyl-acetylated, were also present. We additionally showed that these domains, compared to the broad wall, are less affected by wall modifying activities during a time interval of days. Overall, the protocol and the data presented here open new opportunities for the study of wall polysaccharides associated with PD. Frontiers Media S.A. 2022-10-25 /pmc/articles/PMC9640925/ /pubmed/36388604 http://dx.doi.org/10.3389/fpls.2022.1020506 Text en Copyright © 2022 Paterlini, Sechet, Immel, Grison, Pilard, Pelloux, Mouille, Bayer and Voxeur https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Paterlini, A.
Sechet, J.
Immel, F.
Grison, M. S.
Pilard, S.
Pelloux, J.
Mouille, G.
Bayer, E. M.
Voxeur, A.
Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata
title Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata
title_full Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata
title_fullStr Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata
title_full_unstemmed Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata
title_short Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata
title_sort enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9640925/
https://www.ncbi.nlm.nih.gov/pubmed/36388604
http://dx.doi.org/10.3389/fpls.2022.1020506
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