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Characteristics and biological control functions of Bacillus sp. PM8313 as a host-associated probiotic in red sea bream (Pagrus major) aquaculture
Host-associated probiotics (HAPs) are bacteria originally isolated from rearing water or the host's gastrointestinal tract in order to enhance the host's growth and health. This study investigated the HAP potential of Bacillus sp. PM8313, isolated from wild red sea bream (Pagrus major), th...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9641168/ https://www.ncbi.nlm.nih.gov/pubmed/36381063 http://dx.doi.org/10.1016/j.aninu.2022.08.011 |
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author | Jang, Won Je Lee, Kyung-Bon Jeon, Mi-Hyeon Lee, Su-Jeong Hur, Sang Woo Lee, Seunghan Lee, Bong-Joo Lee, Jong Min Kim, Kang-Woong Lee, Eun-Woo |
author_facet | Jang, Won Je Lee, Kyung-Bon Jeon, Mi-Hyeon Lee, Su-Jeong Hur, Sang Woo Lee, Seunghan Lee, Bong-Joo Lee, Jong Min Kim, Kang-Woong Lee, Eun-Woo |
author_sort | Jang, Won Je |
collection | PubMed |
description | Host-associated probiotics (HAPs) are bacteria originally isolated from rearing water or the host's gastrointestinal tract in order to enhance the host's growth and health. This study investigated the HAP potential of Bacillus sp. PM8313, isolated from wild red sea bream (Pagrus major), through characterization and feeding trials. Results based on in vitro tests showed that PM8313 is safe, confirming its hemolytic, cytotoxic, and antibiotic resistance. In addition, PM8313 showed advantages as a probiotic with high viability in the gastrointestinal model and a high cell adhesion rate. Whole-genome sequencing demonstrated that PM8313 has a 4,615,871 bp single circular chromosome and a guanine–cytosine content of 45.25%. It also showed the absence of genes encoding virulence factors, such as cytotoxin, enterotoxin, hemolysin, sphingomyelinase, and phospholipase. In the feeding trial, a supplemental diet of 1 × 10(8) CFU/g PM8313 positively altered the weight gain, digestive enzyme activity, and intestinal microbiota composition of red sea bream. Analysis of nonspecific immune parameters and immune-related gene expression, and a challenge test showed that PM8313 supplementation increases immunity and pathogenic bacteria resistance. Our findings suggest that PM8313 should be considered for application as a novel HAP to red sea bream aquaculture. |
format | Online Article Text |
id | pubmed-9641168 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-96411682022-11-14 Characteristics and biological control functions of Bacillus sp. PM8313 as a host-associated probiotic in red sea bream (Pagrus major) aquaculture Jang, Won Je Lee, Kyung-Bon Jeon, Mi-Hyeon Lee, Su-Jeong Hur, Sang Woo Lee, Seunghan Lee, Bong-Joo Lee, Jong Min Kim, Kang-Woong Lee, Eun-Woo Anim Nutr Original Research Article Host-associated probiotics (HAPs) are bacteria originally isolated from rearing water or the host's gastrointestinal tract in order to enhance the host's growth and health. This study investigated the HAP potential of Bacillus sp. PM8313, isolated from wild red sea bream (Pagrus major), through characterization and feeding trials. Results based on in vitro tests showed that PM8313 is safe, confirming its hemolytic, cytotoxic, and antibiotic resistance. In addition, PM8313 showed advantages as a probiotic with high viability in the gastrointestinal model and a high cell adhesion rate. Whole-genome sequencing demonstrated that PM8313 has a 4,615,871 bp single circular chromosome and a guanine–cytosine content of 45.25%. It also showed the absence of genes encoding virulence factors, such as cytotoxin, enterotoxin, hemolysin, sphingomyelinase, and phospholipase. In the feeding trial, a supplemental diet of 1 × 10(8) CFU/g PM8313 positively altered the weight gain, digestive enzyme activity, and intestinal microbiota composition of red sea bream. Analysis of nonspecific immune parameters and immune-related gene expression, and a challenge test showed that PM8313 supplementation increases immunity and pathogenic bacteria resistance. Our findings suggest that PM8313 should be considered for application as a novel HAP to red sea bream aquaculture. KeAi Publishing 2022-09-21 /pmc/articles/PMC9641168/ /pubmed/36381063 http://dx.doi.org/10.1016/j.aninu.2022.08.011 Text en © 2022 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Research Article Jang, Won Je Lee, Kyung-Bon Jeon, Mi-Hyeon Lee, Su-Jeong Hur, Sang Woo Lee, Seunghan Lee, Bong-Joo Lee, Jong Min Kim, Kang-Woong Lee, Eun-Woo Characteristics and biological control functions of Bacillus sp. PM8313 as a host-associated probiotic in red sea bream (Pagrus major) aquaculture |
title | Characteristics and biological control functions of Bacillus sp. PM8313 as a host-associated probiotic in red sea bream (Pagrus major) aquaculture |
title_full | Characteristics and biological control functions of Bacillus sp. PM8313 as a host-associated probiotic in red sea bream (Pagrus major) aquaculture |
title_fullStr | Characteristics and biological control functions of Bacillus sp. PM8313 as a host-associated probiotic in red sea bream (Pagrus major) aquaculture |
title_full_unstemmed | Characteristics and biological control functions of Bacillus sp. PM8313 as a host-associated probiotic in red sea bream (Pagrus major) aquaculture |
title_short | Characteristics and biological control functions of Bacillus sp. PM8313 as a host-associated probiotic in red sea bream (Pagrus major) aquaculture |
title_sort | characteristics and biological control functions of bacillus sp. pm8313 as a host-associated probiotic in red sea bream (pagrus major) aquaculture |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9641168/ https://www.ncbi.nlm.nih.gov/pubmed/36381063 http://dx.doi.org/10.1016/j.aninu.2022.08.011 |
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