Protocol for the setup and use of a human choroid plexus endothelial-epithelial two-cell-type in vitro model

Choroid plexus, located in brain ventricles, is the site of blood-cerebrospinal fluid barrier that contains endothelial cells and an epithelial monolayer separated by stroma. We established a two-cell-type model of the human choroid plexus consisting of immortalized endothelial cells (iHCPEnC) and e...

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Detalles Bibliográficos
Autores principales: Herold, Rosanna, Borkowski, Julia, Schwerk, Christian, Stump-Guthier, Carolin, Lampe, Marko, Ishikawa, Hiroshi, Muranyi, Walter, Schroten, Horst
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9641265/
https://www.ncbi.nlm.nih.gov/pubmed/36386888
http://dx.doi.org/10.1016/j.xpro.2022.101816
Descripción
Sumario:Choroid plexus, located in brain ventricles, is the site of blood-cerebrospinal fluid barrier that contains endothelial cells and an epithelial monolayer separated by stroma. We established a two-cell-type model of the human choroid plexus consisting of immortalized endothelial cells (iHCPEnC) and epithelial papilloma (HIBCPP) cells grown on opposite sides of filter supports. In this protocol, we describe the preparation of this model, the measurement of transepithelial electrical resistance (TEER), and immunofluorescence imaging-based analysis to determine the barrier function. For complete details on the use and execution of this protocol, please refer to Muranyi et al. (2022).

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