Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation

It is technically challenging to generate large doses of regulatory T cells (Tregs) engineered to express a chimeric antigen receptor (CAR) in non-human primates (NHP). Here, we have optimized the manufacturing of CAR Tregs by stringent sorting of Tregs, stimulation by artificial antigen-presenting...

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Autores principales: Ellis, Gavin I., Deng, Mosha Z., Winn, Delaine W., Coker, Kimberly E., Shukla, Divanshu, Bhoj, Vijay, Milone, Michael C., Duran-Struuck, Raimon, Riley, James L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9641266/
https://www.ncbi.nlm.nih.gov/pubmed/36386869
http://dx.doi.org/10.1016/j.xpro.2022.101784
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author Ellis, Gavin I.
Deng, Mosha Z.
Winn, Delaine W.
Coker, Kimberly E.
Shukla, Divanshu
Bhoj, Vijay
Milone, Michael C.
Duran-Struuck, Raimon
Riley, James L.
author_facet Ellis, Gavin I.
Deng, Mosha Z.
Winn, Delaine W.
Coker, Kimberly E.
Shukla, Divanshu
Bhoj, Vijay
Milone, Michael C.
Duran-Struuck, Raimon
Riley, James L.
author_sort Ellis, Gavin I.
collection PubMed
description It is technically challenging to generate large doses of regulatory T cells (Tregs) engineered to express a chimeric antigen receptor (CAR) in non-human primates (NHP). Here, we have optimized the manufacturing of CAR Tregs by stringent sorting of Tregs, stimulation by artificial antigen-presenting cells, transduction by simian tropic lentiviral vectors, and antigen-specific expansion. The result of this method is highly suppressive CAR Tregs for use in a pre-clinical, large animal model of transplant tolerance. For complete details on the use and execution of this protocol, please refer to Ellis et al. (2022).
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spelling pubmed-96412662022-11-15 Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation Ellis, Gavin I. Deng, Mosha Z. Winn, Delaine W. Coker, Kimberly E. Shukla, Divanshu Bhoj, Vijay Milone, Michael C. Duran-Struuck, Raimon Riley, James L. STAR Protoc Protocol It is technically challenging to generate large doses of regulatory T cells (Tregs) engineered to express a chimeric antigen receptor (CAR) in non-human primates (NHP). Here, we have optimized the manufacturing of CAR Tregs by stringent sorting of Tregs, stimulation by artificial antigen-presenting cells, transduction by simian tropic lentiviral vectors, and antigen-specific expansion. The result of this method is highly suppressive CAR Tregs for use in a pre-clinical, large animal model of transplant tolerance. For complete details on the use and execution of this protocol, please refer to Ellis et al. (2022). Elsevier 2022-11-05 /pmc/articles/PMC9641266/ /pubmed/36386869 http://dx.doi.org/10.1016/j.xpro.2022.101784 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Ellis, Gavin I.
Deng, Mosha Z.
Winn, Delaine W.
Coker, Kimberly E.
Shukla, Divanshu
Bhoj, Vijay
Milone, Michael C.
Duran-Struuck, Raimon
Riley, James L.
Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
title Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
title_full Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
title_fullStr Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
title_full_unstemmed Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
title_short Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
title_sort generation of non-human primate car tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9641266/
https://www.ncbi.nlm.nih.gov/pubmed/36386869
http://dx.doi.org/10.1016/j.xpro.2022.101784
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