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Mitochondrial DNA barcoding of mosquito species (Diptera: Culicidae) in Thailand
The correct identification of mosquito species is important for effective mosquito vector control. However, the standard morphological identification of mosquito species based on the available keys is not easy with specimens in the field due to missing or damaged morphological features during mosqui...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9642330/ https://www.ncbi.nlm.nih.gov/pubmed/36137118 http://dx.doi.org/10.1371/journal.pone.0275090 |
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author | Chaiphongpachara, Tanawat Changbunjong, Tanasak Laojun, Sedthapong Nutepsu, Teerayoot Suwandittakul, Nantana Kuntawong, Kewarin Sumruayphol, Suchada Ruangsittichai, Jiraporn |
author_facet | Chaiphongpachara, Tanawat Changbunjong, Tanasak Laojun, Sedthapong Nutepsu, Teerayoot Suwandittakul, Nantana Kuntawong, Kewarin Sumruayphol, Suchada Ruangsittichai, Jiraporn |
author_sort | Chaiphongpachara, Tanawat |
collection | PubMed |
description | The correct identification of mosquito species is important for effective mosquito vector control. However, the standard morphological identification of mosquito species based on the available keys is not easy with specimens in the field due to missing or damaged morphological features during mosquito collections, often leading to the misidentification of morphologically indistinguishable. To resolve this problem, we collected mosquito species across Thailand to gather genetic information, and evaluated the DNA barcoding efficacy for mosquito species identification in Thailand. A total of 310 mosquito samples, representing 73 mosquito species, were amplified using mitochondrial cytochrome c oxidase subunit I (COI) primers. The average maximum intraspecific genetic variation of the 73 mosquito species was 1% ranged from 0–5.7%. While, average minimum interspecific genetic variation (the distance to the nearest neighbour) of the 73 mosquito species was 7% ranged from 0.3–12.9%. The identification of success rates based on the “Best Match,” “Best Close Match,” and “All Species Barcodes” methods were 97.7%, 91.6%, and 81%, respectively. Phylogenetic analyses of Anopheles COI sequences demonstrated a clear separation between almost all species (except for those between An. baimaii and An. dirus), with high bootstrap support values (97%–99%). Furthermore, phylogenetic analyses revealed potential sibling species of An. annularis, An. tessellatus, and An. subpictus in Thailand. Our results indicated that DNA barcoding is an effective molecular approach for the accurate identification of mosquitoes in Thailand. |
format | Online Article Text |
id | pubmed-9642330 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-96423302022-11-15 Mitochondrial DNA barcoding of mosquito species (Diptera: Culicidae) in Thailand Chaiphongpachara, Tanawat Changbunjong, Tanasak Laojun, Sedthapong Nutepsu, Teerayoot Suwandittakul, Nantana Kuntawong, Kewarin Sumruayphol, Suchada Ruangsittichai, Jiraporn PLoS One Research Article The correct identification of mosquito species is important for effective mosquito vector control. However, the standard morphological identification of mosquito species based on the available keys is not easy with specimens in the field due to missing or damaged morphological features during mosquito collections, often leading to the misidentification of morphologically indistinguishable. To resolve this problem, we collected mosquito species across Thailand to gather genetic information, and evaluated the DNA barcoding efficacy for mosquito species identification in Thailand. A total of 310 mosquito samples, representing 73 mosquito species, were amplified using mitochondrial cytochrome c oxidase subunit I (COI) primers. The average maximum intraspecific genetic variation of the 73 mosquito species was 1% ranged from 0–5.7%. While, average minimum interspecific genetic variation (the distance to the nearest neighbour) of the 73 mosquito species was 7% ranged from 0.3–12.9%. The identification of success rates based on the “Best Match,” “Best Close Match,” and “All Species Barcodes” methods were 97.7%, 91.6%, and 81%, respectively. Phylogenetic analyses of Anopheles COI sequences demonstrated a clear separation between almost all species (except for those between An. baimaii and An. dirus), with high bootstrap support values (97%–99%). Furthermore, phylogenetic analyses revealed potential sibling species of An. annularis, An. tessellatus, and An. subpictus in Thailand. Our results indicated that DNA barcoding is an effective molecular approach for the accurate identification of mosquitoes in Thailand. Public Library of Science 2022-09-22 /pmc/articles/PMC9642330/ /pubmed/36137118 http://dx.doi.org/10.1371/journal.pone.0275090 Text en © 2022 Chaiphongpachara et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Chaiphongpachara, Tanawat Changbunjong, Tanasak Laojun, Sedthapong Nutepsu, Teerayoot Suwandittakul, Nantana Kuntawong, Kewarin Sumruayphol, Suchada Ruangsittichai, Jiraporn Mitochondrial DNA barcoding of mosquito species (Diptera: Culicidae) in Thailand |
title | Mitochondrial DNA barcoding of mosquito species (Diptera: Culicidae)
in Thailand |
title_full | Mitochondrial DNA barcoding of mosquito species (Diptera: Culicidae)
in Thailand |
title_fullStr | Mitochondrial DNA barcoding of mosquito species (Diptera: Culicidae)
in Thailand |
title_full_unstemmed | Mitochondrial DNA barcoding of mosquito species (Diptera: Culicidae)
in Thailand |
title_short | Mitochondrial DNA barcoding of mosquito species (Diptera: Culicidae)
in Thailand |
title_sort | mitochondrial dna barcoding of mosquito species (diptera: culicidae)
in thailand |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9642330/ https://www.ncbi.nlm.nih.gov/pubmed/36137118 http://dx.doi.org/10.1371/journal.pone.0275090 |
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