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The variants of polymyxin susceptibility in different species of genus Aeromonas
The aquatic environment is an important medium for the accumulation and dissemination of antibiotic-resistant bacteria as it is often closely related to human activities. Previous studies paid little attention to the prevalence and mechanism of polymyxin-resistant bacteria in the aquatic environment...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9642839/ https://www.ncbi.nlm.nih.gov/pubmed/36386612 http://dx.doi.org/10.3389/fmicb.2022.1030564 |
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author | Xu, Linna Fan, Junfeng Fu, Hao Yang, Yuyi Luo, Qixia Wan, Fen |
author_facet | Xu, Linna Fan, Junfeng Fu, Hao Yang, Yuyi Luo, Qixia Wan, Fen |
author_sort | Xu, Linna |
collection | PubMed |
description | The aquatic environment is an important medium for the accumulation and dissemination of antibiotic-resistant bacteria as it is often closely related to human activities. Previous studies paid little attention to the prevalence and mechanism of polymyxin-resistant bacteria in the aquatic environment. As a Gram-negative opportunistic pathogen widely distributed in aquatic ecosystems, the antibiotic-resistant profile of Aeromonas spp. deserves much attention. In this study, we identified 61 Aeromonas spp. isolates from water samples in the section of the Yangtze River. The total polymyxin B (PMB) resistance rate of these strains was 49.18% (30/61), showing a high level of polymyxin resistance in Aeromonas spp. The MIC(50) and MIC(90) for PMB exhibited a significant discrepancy among different species (p < 0.001). The MIC(50) and MIC(90) for PMB in the Aeromonas hydrophila were 128 mg/L and above 128 mg/L while in Aeromonas caviae and Aeromonas veronii, the MIC(50) and MIC(90) value were both 2 mg/L. Only two A. veronii strains (MIC = 2 mg/L) and one A. caviae strain (MIC = 0.5 mg/L) were identified as carrying mobilized polymyxin resistant gene mcr-3.42, and mcr-3.16. All mcr genes were located in the chromosome. This is the first report that the downstream region of mcr-3.42 was the truncated mcr-3-like gene separated by the insertion sequences of ISAs20 (1,674 bp) and ISAs2 (1,084 bp). Analysis of epidemiology of mcr-positive Aeromonas genomes from GenBank database showed that the genus Aeromonas and the aquatic environment might be the potential container and reservoir of mcr-3. By the whole-genome sequencing and qRT-PCR, we inferred that the sequence differences in the AAA domain of MlaF protein and its expression level among these three species might be involved in the development of polymyxin resistance. Our study provided evidences of the possible mechanism for the variety of polymyxin susceptibility in different species of the genus Aeromonas and a theoretical basis for the surveillance of the aquatic environment. |
format | Online Article Text |
id | pubmed-9642839 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96428392022-11-15 The variants of polymyxin susceptibility in different species of genus Aeromonas Xu, Linna Fan, Junfeng Fu, Hao Yang, Yuyi Luo, Qixia Wan, Fen Front Microbiol Microbiology The aquatic environment is an important medium for the accumulation and dissemination of antibiotic-resistant bacteria as it is often closely related to human activities. Previous studies paid little attention to the prevalence and mechanism of polymyxin-resistant bacteria in the aquatic environment. As a Gram-negative opportunistic pathogen widely distributed in aquatic ecosystems, the antibiotic-resistant profile of Aeromonas spp. deserves much attention. In this study, we identified 61 Aeromonas spp. isolates from water samples in the section of the Yangtze River. The total polymyxin B (PMB) resistance rate of these strains was 49.18% (30/61), showing a high level of polymyxin resistance in Aeromonas spp. The MIC(50) and MIC(90) for PMB exhibited a significant discrepancy among different species (p < 0.001). The MIC(50) and MIC(90) for PMB in the Aeromonas hydrophila were 128 mg/L and above 128 mg/L while in Aeromonas caviae and Aeromonas veronii, the MIC(50) and MIC(90) value were both 2 mg/L. Only two A. veronii strains (MIC = 2 mg/L) and one A. caviae strain (MIC = 0.5 mg/L) were identified as carrying mobilized polymyxin resistant gene mcr-3.42, and mcr-3.16. All mcr genes were located in the chromosome. This is the first report that the downstream region of mcr-3.42 was the truncated mcr-3-like gene separated by the insertion sequences of ISAs20 (1,674 bp) and ISAs2 (1,084 bp). Analysis of epidemiology of mcr-positive Aeromonas genomes from GenBank database showed that the genus Aeromonas and the aquatic environment might be the potential container and reservoir of mcr-3. By the whole-genome sequencing and qRT-PCR, we inferred that the sequence differences in the AAA domain of MlaF protein and its expression level among these three species might be involved in the development of polymyxin resistance. Our study provided evidences of the possible mechanism for the variety of polymyxin susceptibility in different species of the genus Aeromonas and a theoretical basis for the surveillance of the aquatic environment. Frontiers Media S.A. 2022-10-25 /pmc/articles/PMC9642839/ /pubmed/36386612 http://dx.doi.org/10.3389/fmicb.2022.1030564 Text en Copyright © 2022 Xu, Fan, Fu, Yang, Luo and Wan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Xu, Linna Fan, Junfeng Fu, Hao Yang, Yuyi Luo, Qixia Wan, Fen The variants of polymyxin susceptibility in different species of genus Aeromonas |
title | The variants of polymyxin susceptibility in different species of genus Aeromonas |
title_full | The variants of polymyxin susceptibility in different species of genus Aeromonas |
title_fullStr | The variants of polymyxin susceptibility in different species of genus Aeromonas |
title_full_unstemmed | The variants of polymyxin susceptibility in different species of genus Aeromonas |
title_short | The variants of polymyxin susceptibility in different species of genus Aeromonas |
title_sort | variants of polymyxin susceptibility in different species of genus aeromonas |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9642839/ https://www.ncbi.nlm.nih.gov/pubmed/36386612 http://dx.doi.org/10.3389/fmicb.2022.1030564 |
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