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Application of the RAPD technique to identify genetic diversity in cultivated forms of Capsicum annuum L.

BACKGROUND: The extensive use of pepper fruit creates a constant demand for new cultivars with specific agromorphological properties. The wide variety of breeding materials of this species means that methods based on morphological traits descriptions are not always sufficient to allow for their iden...

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Autores principales: Niklas, Aleksandra, Olszewska, Dorota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9642924/
https://www.ncbi.nlm.nih.gov/pubmed/36606024
http://dx.doi.org/10.5114/bta.2021.106523
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author Niklas, Aleksandra
Olszewska, Dorota
author_facet Niklas, Aleksandra
Olszewska, Dorota
author_sort Niklas, Aleksandra
collection PubMed
description BACKGROUND: The extensive use of pepper fruit creates a constant demand for new cultivars with specific agromorphological properties. The wide variety of breeding materials of this species means that methods based on morphological traits descriptions are not always sufficient to allow for their identification. Genetic homogeneity must be guaranteed to ensure repeatability of phenotypic traits. Most often, molecular analyses characterizing diversity at the DNA level are used for this purpose. MATERIAL AND METHODS: The PCR-RAPD technique was used for molecular analysis of the generative offspring of three cultivars of pepper: “Anchi”, “Luba” and “Sono” and their forms of different fruit colour. The genetic distance between the tested genotypes was determined using the Nei and Li formula. RESULTS: The reaction with 26 RAPD primers resulted in a total of 262 products and 5.2% of them were polymorphic bands. Eight of the used primers generated 12 polymorphic products that differentiated the tested genotypes. The “Anchi” cultivar was identified by the primers A07, K10, Q07 and AE10. Starter Q07 identified as well the “Luba” cultivar. Reactions carried out with primers B10 and RAD1 identified the “Sono” cultivar. In addition, primer A15 generated products that made it possible to distinguish yellow-fruit and red-fruit forms within the “Luba” and “Sono” cultivars. CONCLUSION: The analyses showed a low degree of genetic distance between C. annuum L. cultivars confirming the genetic homogeneity of the examined groups of plants and creating and opportunity for molecular identification of the genetic diversity within the “Luba” and “Sono” cultivars.
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spelling pubmed-96429242023-01-04 Application of the RAPD technique to identify genetic diversity in cultivated forms of Capsicum annuum L. Niklas, Aleksandra Olszewska, Dorota BioTechnologia (Pozn) Research Papers BACKGROUND: The extensive use of pepper fruit creates a constant demand for new cultivars with specific agromorphological properties. The wide variety of breeding materials of this species means that methods based on morphological traits descriptions are not always sufficient to allow for their identification. Genetic homogeneity must be guaranteed to ensure repeatability of phenotypic traits. Most often, molecular analyses characterizing diversity at the DNA level are used for this purpose. MATERIAL AND METHODS: The PCR-RAPD technique was used for molecular analysis of the generative offspring of three cultivars of pepper: “Anchi”, “Luba” and “Sono” and their forms of different fruit colour. The genetic distance between the tested genotypes was determined using the Nei and Li formula. RESULTS: The reaction with 26 RAPD primers resulted in a total of 262 products and 5.2% of them were polymorphic bands. Eight of the used primers generated 12 polymorphic products that differentiated the tested genotypes. The “Anchi” cultivar was identified by the primers A07, K10, Q07 and AE10. Starter Q07 identified as well the “Luba” cultivar. Reactions carried out with primers B10 and RAD1 identified the “Sono” cultivar. In addition, primer A15 generated products that made it possible to distinguish yellow-fruit and red-fruit forms within the “Luba” and “Sono” cultivars. CONCLUSION: The analyses showed a low degree of genetic distance between C. annuum L. cultivars confirming the genetic homogeneity of the examined groups of plants and creating and opportunity for molecular identification of the genetic diversity within the “Luba” and “Sono” cultivars. Termedia Publishing House 2021-07-05 /pmc/articles/PMC9642924/ /pubmed/36606024 http://dx.doi.org/10.5114/bta.2021.106523 Text en © 2021 Institute of Bioorganic Chemistry, Polish Academy of Sciences https://creativecommons.org/licenses/by-nc-nd/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs (CC BY-NC-ND), allowing third parties to download and share its works but not commercially purposes or to create derivative works.
spellingShingle Research Papers
Niklas, Aleksandra
Olszewska, Dorota
Application of the RAPD technique to identify genetic diversity in cultivated forms of Capsicum annuum L.
title Application of the RAPD technique to identify genetic diversity in cultivated forms of Capsicum annuum L.
title_full Application of the RAPD technique to identify genetic diversity in cultivated forms of Capsicum annuum L.
title_fullStr Application of the RAPD technique to identify genetic diversity in cultivated forms of Capsicum annuum L.
title_full_unstemmed Application of the RAPD technique to identify genetic diversity in cultivated forms of Capsicum annuum L.
title_short Application of the RAPD technique to identify genetic diversity in cultivated forms of Capsicum annuum L.
title_sort application of the rapd technique to identify genetic diversity in cultivated forms of capsicum annuum l.
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9642924/
https://www.ncbi.nlm.nih.gov/pubmed/36606024
http://dx.doi.org/10.5114/bta.2021.106523
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