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In vitro regeneration of Vitellaria paradoxa from shoot tip explants

Shea tree (Vitellaria paradoxa) is an essential tree crop with great potential economic value mainly because of its seed oil (shea butter) which is of high demand for manufacturing assorted products in food, cosmetic, and rubber industries. Propagation of this species is, however, hindered by relati...

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Autores principales: Afful, Nusrat Tsemah, Abdulai, Iddrisu, Azu, Elaine, Elegba, Wilfred, Annor, Clement, Akama, Christian, Asare, Kwame, Dentey, John, Amoatey, Harry M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9642945/
https://www.ncbi.nlm.nih.gov/pubmed/36605379
http://dx.doi.org/10.5114/bta.2022.113917
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author Afful, Nusrat Tsemah
Abdulai, Iddrisu
Azu, Elaine
Elegba, Wilfred
Annor, Clement
Akama, Christian
Asare, Kwame
Dentey, John
Amoatey, Harry M.
author_facet Afful, Nusrat Tsemah
Abdulai, Iddrisu
Azu, Elaine
Elegba, Wilfred
Annor, Clement
Akama, Christian
Asare, Kwame
Dentey, John
Amoatey, Harry M.
author_sort Afful, Nusrat Tsemah
collection PubMed
description Shea tree (Vitellaria paradoxa) is an essential tree crop with great potential economic value mainly because of its seed oil (shea butter) which is of high demand for manufacturing assorted products in food, cosmetic, and rubber industries. Propagation of this species is, however, hindered by relative unavailability of seed (nuts), erratic seed germination, a long vegetative phase, and latex exudation from cuttings. Thus, another method of propagation through in vitro culture is recommended for rapid multiplication of shea genotypes for large-scale cultivation. In the present study, the effects of two cytokinins, namely, 6-benzylaminopurine (BAP) and kinetin (KIN), and one auxin, namely 1-naphthaleneacetic acid (NAA), on shoot and/or root induction in vitro were assessed at various combinations/concentrations. The inclusion of these growth regulators in the culture medium significantly improved (P < 0.05) shoot/root regeneration over the controls. The highest shoot regeneration percentage (100%) was obtained on Murashige and Skoog (MS) basal medium supplemented with 2 mg ∙ dm(−3) KIN + + 0.5 mg ∙ dm(−3) NAA or 1.5 mg ∙ dm(−3) KIN within 7/8 days of inoculation. This medium (2 mg ∙ dm(−3) KIN + + 0.5 mg ∙ dm(−3) NAA) showed the highest mean shoot length of 3.24 cm. Compared to KIN, BAP was more effective in inducing vigorous shoot growth. However, rooting was induced only on MS medium modified with 1 mg ∙ dm(−3) BAP + 0.5 mg ∙ dm(−3) NAA. These findings can serve as baseline information for in vitro, commercialscale propagation of shea tree.
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spelling pubmed-96429452023-01-04 In vitro regeneration of Vitellaria paradoxa from shoot tip explants Afful, Nusrat Tsemah Abdulai, Iddrisu Azu, Elaine Elegba, Wilfred Annor, Clement Akama, Christian Asare, Kwame Dentey, John Amoatey, Harry M. BioTechnologia (Pozn) Research Papers Shea tree (Vitellaria paradoxa) is an essential tree crop with great potential economic value mainly because of its seed oil (shea butter) which is of high demand for manufacturing assorted products in food, cosmetic, and rubber industries. Propagation of this species is, however, hindered by relative unavailability of seed (nuts), erratic seed germination, a long vegetative phase, and latex exudation from cuttings. Thus, another method of propagation through in vitro culture is recommended for rapid multiplication of shea genotypes for large-scale cultivation. In the present study, the effects of two cytokinins, namely, 6-benzylaminopurine (BAP) and kinetin (KIN), and one auxin, namely 1-naphthaleneacetic acid (NAA), on shoot and/or root induction in vitro were assessed at various combinations/concentrations. The inclusion of these growth regulators in the culture medium significantly improved (P < 0.05) shoot/root regeneration over the controls. The highest shoot regeneration percentage (100%) was obtained on Murashige and Skoog (MS) basal medium supplemented with 2 mg ∙ dm(−3) KIN + + 0.5 mg ∙ dm(−3) NAA or 1.5 mg ∙ dm(−3) KIN within 7/8 days of inoculation. This medium (2 mg ∙ dm(−3) KIN + + 0.5 mg ∙ dm(−3) NAA) showed the highest mean shoot length of 3.24 cm. Compared to KIN, BAP was more effective in inducing vigorous shoot growth. However, rooting was induced only on MS medium modified with 1 mg ∙ dm(−3) BAP + 0.5 mg ∙ dm(−3) NAA. These findings can serve as baseline information for in vitro, commercialscale propagation of shea tree. Termedia Publishing House 2022-03-24 /pmc/articles/PMC9642945/ /pubmed/36605379 http://dx.doi.org/10.5114/bta.2022.113917 Text en © 2022 Institute of Bioorganic Chemistry, Polish Academy of Sciences https://creativecommons.org/licenses/by-nc-nd/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs (CC BY-NC-ND), allowing third parties to download and share its works but not commercially purposes or to create derivative works.
spellingShingle Research Papers
Afful, Nusrat Tsemah
Abdulai, Iddrisu
Azu, Elaine
Elegba, Wilfred
Annor, Clement
Akama, Christian
Asare, Kwame
Dentey, John
Amoatey, Harry M.
In vitro regeneration of Vitellaria paradoxa from shoot tip explants
title In vitro regeneration of Vitellaria paradoxa from shoot tip explants
title_full In vitro regeneration of Vitellaria paradoxa from shoot tip explants
title_fullStr In vitro regeneration of Vitellaria paradoxa from shoot tip explants
title_full_unstemmed In vitro regeneration of Vitellaria paradoxa from shoot tip explants
title_short In vitro regeneration of Vitellaria paradoxa from shoot tip explants
title_sort in vitro regeneration of vitellaria paradoxa from shoot tip explants
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9642945/
https://www.ncbi.nlm.nih.gov/pubmed/36605379
http://dx.doi.org/10.5114/bta.2022.113917
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