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Reliable reference genes and abiotic stress marker genes in Klebsormidium nitens
Microalgae have recently emerged as a key research topic, especially as biological models. Among them, the green alga Klebsormidium nitens, thanks to its particular adaptation to environmental stresses, represents an interesting photosynthetic eukaryote for studying the transition stages leading to...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9643330/ https://www.ncbi.nlm.nih.gov/pubmed/36348043 http://dx.doi.org/10.1038/s41598-022-23783-9 |
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author | Chatelain, Pauline Blanchard, Cécile Astier, Jeremy Klinguer, Agnès Wendehenne, David Jeandroz, Sylvain Rosnoblet, Claire |
author_facet | Chatelain, Pauline Blanchard, Cécile Astier, Jeremy Klinguer, Agnès Wendehenne, David Jeandroz, Sylvain Rosnoblet, Claire |
author_sort | Chatelain, Pauline |
collection | PubMed |
description | Microalgae have recently emerged as a key research topic, especially as biological models. Among them, the green alga Klebsormidium nitens, thanks to its particular adaptation to environmental stresses, represents an interesting photosynthetic eukaryote for studying the transition stages leading to the colonization of terrestrial life. The tolerance to different stresses is manifested by changes in gene expression, which can be monitored by quantifying the amounts of transcripts by RT-qPCR. The identification of optimal reference genes for experiment normalization was therefore necessary. In this study, using four statistical algorithms followed by the RankAggreg package, we determined the best reference gene pairs suitable for normalizing RT-qPCR data in K. nitens in response to three abiotic stresses: high salinity, PEG-induced dehydration and heat shock. Based on these reference genes, we were able to identify marker genes in response to the three abiotic stresses in K. nitens. |
format | Online Article Text |
id | pubmed-9643330 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-96433302022-11-15 Reliable reference genes and abiotic stress marker genes in Klebsormidium nitens Chatelain, Pauline Blanchard, Cécile Astier, Jeremy Klinguer, Agnès Wendehenne, David Jeandroz, Sylvain Rosnoblet, Claire Sci Rep Article Microalgae have recently emerged as a key research topic, especially as biological models. Among them, the green alga Klebsormidium nitens, thanks to its particular adaptation to environmental stresses, represents an interesting photosynthetic eukaryote for studying the transition stages leading to the colonization of terrestrial life. The tolerance to different stresses is manifested by changes in gene expression, which can be monitored by quantifying the amounts of transcripts by RT-qPCR. The identification of optimal reference genes for experiment normalization was therefore necessary. In this study, using four statistical algorithms followed by the RankAggreg package, we determined the best reference gene pairs suitable for normalizing RT-qPCR data in K. nitens in response to three abiotic stresses: high salinity, PEG-induced dehydration and heat shock. Based on these reference genes, we were able to identify marker genes in response to the three abiotic stresses in K. nitens. Nature Publishing Group UK 2022-11-08 /pmc/articles/PMC9643330/ /pubmed/36348043 http://dx.doi.org/10.1038/s41598-022-23783-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Chatelain, Pauline Blanchard, Cécile Astier, Jeremy Klinguer, Agnès Wendehenne, David Jeandroz, Sylvain Rosnoblet, Claire Reliable reference genes and abiotic stress marker genes in Klebsormidium nitens |
title | Reliable reference genes and abiotic stress marker genes in Klebsormidium nitens |
title_full | Reliable reference genes and abiotic stress marker genes in Klebsormidium nitens |
title_fullStr | Reliable reference genes and abiotic stress marker genes in Klebsormidium nitens |
title_full_unstemmed | Reliable reference genes and abiotic stress marker genes in Klebsormidium nitens |
title_short | Reliable reference genes and abiotic stress marker genes in Klebsormidium nitens |
title_sort | reliable reference genes and abiotic stress marker genes in klebsormidium nitens |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9643330/ https://www.ncbi.nlm.nih.gov/pubmed/36348043 http://dx.doi.org/10.1038/s41598-022-23783-9 |
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