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Acellular Vascular Scaffolds Preloaded With Heparin and Hepatocyte Growth Factor for Small-Diameter Vascular Grafts Might Inhibit Intimal Hyperplasia
To develop small-diameter (<6 mm) scaffolds capable of accelerating rapid endothelialization and improving long-term patency rate, we created acellular vascular scaffolds preloaded with heparin and hepatocyte growth factor (HGF). Heparin was conjugated to suppress thrombogenic responses, and HGF...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9643765/ https://www.ncbi.nlm.nih.gov/pubmed/36341505 http://dx.doi.org/10.1177/09636897221134541 |
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author | Cai, Zhiwen Tan, Zhengli Tian, Ran Chen, Xin Miao, Peng Yao, Chenliang Wang, Cong Yu, Zhengya Gu, Yongquan |
author_facet | Cai, Zhiwen Tan, Zhengli Tian, Ran Chen, Xin Miao, Peng Yao, Chenliang Wang, Cong Yu, Zhengya Gu, Yongquan |
author_sort | Cai, Zhiwen |
collection | PubMed |
description | To develop small-diameter (<6 mm) scaffolds capable of accelerating rapid endothelialization and improving long-term patency rate, we created acellular vascular scaffolds preloaded with heparin and hepatocyte growth factor (HGF). Heparin was conjugated to suppress thrombogenic responses, and HGF was immobilized to induce endothelial cells (ECs) proliferation and migration. The scaffolds immobilized with heparin exhibited highly effective localization and sustained release of HGF for 30 days in vitro. We implanted this modified scaffold into the carotid artery of a rabbit model to investigate the efficacy in vivo. The acellular vascular scaffold with heparin only was used as control. After transplantation, the patency of this modified scaffold was 91.67% at 1, 3, 6, and 12 months, while the patency rate in the group with grafted heparin only was 83.33% at 1, 3, 6, and 12 months. This modified scaffold significantly stimulated ECs proliferation and the endothelium aligned in the direction of flow after 12 months. In addition, intimal hyperplasia was significantly reduced in the grafts coated with HGF compared with the control grafts. The small-diameter vascular grafts with an inner diameter of 2.5 mm preloaded with heparin and HGF may be a substitute for autologous blood vessels in clinic. |
format | Online Article Text |
id | pubmed-9643765 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-96437652022-11-15 Acellular Vascular Scaffolds Preloaded With Heparin and Hepatocyte Growth Factor for Small-Diameter Vascular Grafts Might Inhibit Intimal Hyperplasia Cai, Zhiwen Tan, Zhengli Tian, Ran Chen, Xin Miao, Peng Yao, Chenliang Wang, Cong Yu, Zhengya Gu, Yongquan Cell Transplant Original Article To develop small-diameter (<6 mm) scaffolds capable of accelerating rapid endothelialization and improving long-term patency rate, we created acellular vascular scaffolds preloaded with heparin and hepatocyte growth factor (HGF). Heparin was conjugated to suppress thrombogenic responses, and HGF was immobilized to induce endothelial cells (ECs) proliferation and migration. The scaffolds immobilized with heparin exhibited highly effective localization and sustained release of HGF for 30 days in vitro. We implanted this modified scaffold into the carotid artery of a rabbit model to investigate the efficacy in vivo. The acellular vascular scaffold with heparin only was used as control. After transplantation, the patency of this modified scaffold was 91.67% at 1, 3, 6, and 12 months, while the patency rate in the group with grafted heparin only was 83.33% at 1, 3, 6, and 12 months. This modified scaffold significantly stimulated ECs proliferation and the endothelium aligned in the direction of flow after 12 months. In addition, intimal hyperplasia was significantly reduced in the grafts coated with HGF compared with the control grafts. The small-diameter vascular grafts with an inner diameter of 2.5 mm preloaded with heparin and HGF may be a substitute for autologous blood vessels in clinic. SAGE Publications 2022-11-06 /pmc/articles/PMC9643765/ /pubmed/36341505 http://dx.doi.org/10.1177/09636897221134541 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Cai, Zhiwen Tan, Zhengli Tian, Ran Chen, Xin Miao, Peng Yao, Chenliang Wang, Cong Yu, Zhengya Gu, Yongquan Acellular Vascular Scaffolds Preloaded With Heparin and Hepatocyte Growth Factor for Small-Diameter Vascular Grafts Might Inhibit Intimal Hyperplasia |
title | Acellular Vascular Scaffolds Preloaded With Heparin and Hepatocyte
Growth Factor for Small-Diameter Vascular Grafts Might Inhibit Intimal
Hyperplasia |
title_full | Acellular Vascular Scaffolds Preloaded With Heparin and Hepatocyte
Growth Factor for Small-Diameter Vascular Grafts Might Inhibit Intimal
Hyperplasia |
title_fullStr | Acellular Vascular Scaffolds Preloaded With Heparin and Hepatocyte
Growth Factor for Small-Diameter Vascular Grafts Might Inhibit Intimal
Hyperplasia |
title_full_unstemmed | Acellular Vascular Scaffolds Preloaded With Heparin and Hepatocyte
Growth Factor for Small-Diameter Vascular Grafts Might Inhibit Intimal
Hyperplasia |
title_short | Acellular Vascular Scaffolds Preloaded With Heparin and Hepatocyte
Growth Factor for Small-Diameter Vascular Grafts Might Inhibit Intimal
Hyperplasia |
title_sort | acellular vascular scaffolds preloaded with heparin and hepatocyte
growth factor for small-diameter vascular grafts might inhibit intimal
hyperplasia |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9643765/ https://www.ncbi.nlm.nih.gov/pubmed/36341505 http://dx.doi.org/10.1177/09636897221134541 |
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