Cargando…

Modulation of STAT-1, STAT-3, and STAT-6 activities in THP-1 derived macrophages infected with two Trypanosoma cruzi strains

Trypanosoma cruzi is the causative protozoan of Chagas’ Disease, a neglected tropical disease that affects 6−7 million people worldwide. Interaction of the parasite with the host immune system is a key factor in disease progression and chronic symptoms. Although the human immune system is capable of...

Descripción completa

Detalles Bibliográficos
Autores principales: Oliveira, Melissa Martins, Bonturi, Camila Ramalho, Salu, Bruno Ramos, Oliva, Maria Luiza Vilela, Mortara, Renato Arruda, Orikaza, Cristina Mary
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9643828/
https://www.ncbi.nlm.nih.gov/pubmed/36389843
http://dx.doi.org/10.3389/fimmu.2022.1038332
_version_ 1784826605254737920
author Oliveira, Melissa Martins
Bonturi, Camila Ramalho
Salu, Bruno Ramos
Oliva, Maria Luiza Vilela
Mortara, Renato Arruda
Orikaza, Cristina Mary
author_facet Oliveira, Melissa Martins
Bonturi, Camila Ramalho
Salu, Bruno Ramos
Oliva, Maria Luiza Vilela
Mortara, Renato Arruda
Orikaza, Cristina Mary
author_sort Oliveira, Melissa Martins
collection PubMed
description Trypanosoma cruzi is the causative protozoan of Chagas’ Disease, a neglected tropical disease that affects 6−7 million people worldwide. Interaction of the parasite with the host immune system is a key factor in disease progression and chronic symptoms. Although the human immune system is capable of controlling the disease, the parasite has numerous evasion mechanisms that aim to maintain intracellular persistence and survival. Due to the pronounced genetic variability of T. cruzi, co-infections or mixed infections with more than one parasite strain have been reported in the literature. The intermodulation in such cases is unclear. This study aimed to evaluate the co-infection of T. cruzi strains G and CL compared to their individual infections in human macrophages derived from THP-1 cells activated by classical or alternative pathways. Flow cytometry analysis demonstrated that trypomastigotes were more infective than extracellular amastigotes (EAs) and that strain G could infect more macrophages than strain CL. Classically activated macrophages showed lower number of infected cells and IL-4-stimulated cells displayed increased CL-infected macrophages. However, co-infection was a rare event. CL EAs decreased the production of reactive oxygen species (ROS), whereas G trypomastigotes displayed increased ROS detection in classically activated cells. Co-infection did not affect ROS production. Monoinfection by strain G or CL mainly induced an anti-inflammatory cytokine profile by decreasing inflammatory cytokines (IFN-γ, TNF-α, IL-1β) and/or increasing IL-4, IL-10, and TGF-β. Co-infection led to a predominant inflammatory milieu, with reduced IL-10 and TGF-β, and/or promotion of IFN-γ and IL-1β release. Infection by strain G reduced activation of intracellular signal transducer and activator of transcription (STAT) factors. In EAs, monoinfections impaired STAT-1 activity and promoted phosphorylation of STAT-3, both changes may prolong cell survival. Coinfected macrophages displayed pronounced activation of all STATs examined. These activations likely promoted parasite persistence and survival of infected cells. The collective results demonstrate that although macrophages respond to both strains, T. cruzi can modulate the intracellular environment, inducing different responses depending on the strain, parasite infective form, and co-infection or monoinfection. The modulation influences parasite persistence and survival of infected cells.
format Online
Article
Text
id pubmed-9643828
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-96438282022-11-15 Modulation of STAT-1, STAT-3, and STAT-6 activities in THP-1 derived macrophages infected with two Trypanosoma cruzi strains Oliveira, Melissa Martins Bonturi, Camila Ramalho Salu, Bruno Ramos Oliva, Maria Luiza Vilela Mortara, Renato Arruda Orikaza, Cristina Mary Front Immunol Immunology Trypanosoma cruzi is the causative protozoan of Chagas’ Disease, a neglected tropical disease that affects 6−7 million people worldwide. Interaction of the parasite with the host immune system is a key factor in disease progression and chronic symptoms. Although the human immune system is capable of controlling the disease, the parasite has numerous evasion mechanisms that aim to maintain intracellular persistence and survival. Due to the pronounced genetic variability of T. cruzi, co-infections or mixed infections with more than one parasite strain have been reported in the literature. The intermodulation in such cases is unclear. This study aimed to evaluate the co-infection of T. cruzi strains G and CL compared to their individual infections in human macrophages derived from THP-1 cells activated by classical or alternative pathways. Flow cytometry analysis demonstrated that trypomastigotes were more infective than extracellular amastigotes (EAs) and that strain G could infect more macrophages than strain CL. Classically activated macrophages showed lower number of infected cells and IL-4-stimulated cells displayed increased CL-infected macrophages. However, co-infection was a rare event. CL EAs decreased the production of reactive oxygen species (ROS), whereas G trypomastigotes displayed increased ROS detection in classically activated cells. Co-infection did not affect ROS production. Monoinfection by strain G or CL mainly induced an anti-inflammatory cytokine profile by decreasing inflammatory cytokines (IFN-γ, TNF-α, IL-1β) and/or increasing IL-4, IL-10, and TGF-β. Co-infection led to a predominant inflammatory milieu, with reduced IL-10 and TGF-β, and/or promotion of IFN-γ and IL-1β release. Infection by strain G reduced activation of intracellular signal transducer and activator of transcription (STAT) factors. In EAs, monoinfections impaired STAT-1 activity and promoted phosphorylation of STAT-3, both changes may prolong cell survival. Coinfected macrophages displayed pronounced activation of all STATs examined. These activations likely promoted parasite persistence and survival of infected cells. The collective results demonstrate that although macrophages respond to both strains, T. cruzi can modulate the intracellular environment, inducing different responses depending on the strain, parasite infective form, and co-infection or monoinfection. The modulation influences parasite persistence and survival of infected cells. Frontiers Media S.A. 2022-10-26 /pmc/articles/PMC9643828/ /pubmed/36389843 http://dx.doi.org/10.3389/fimmu.2022.1038332 Text en Copyright © 2022 Oliveira, Bonturi, Salu, Oliva, Mortara and Orikaza https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Oliveira, Melissa Martins
Bonturi, Camila Ramalho
Salu, Bruno Ramos
Oliva, Maria Luiza Vilela
Mortara, Renato Arruda
Orikaza, Cristina Mary
Modulation of STAT-1, STAT-3, and STAT-6 activities in THP-1 derived macrophages infected with two Trypanosoma cruzi strains
title Modulation of STAT-1, STAT-3, and STAT-6 activities in THP-1 derived macrophages infected with two Trypanosoma cruzi strains
title_full Modulation of STAT-1, STAT-3, and STAT-6 activities in THP-1 derived macrophages infected with two Trypanosoma cruzi strains
title_fullStr Modulation of STAT-1, STAT-3, and STAT-6 activities in THP-1 derived macrophages infected with two Trypanosoma cruzi strains
title_full_unstemmed Modulation of STAT-1, STAT-3, and STAT-6 activities in THP-1 derived macrophages infected with two Trypanosoma cruzi strains
title_short Modulation of STAT-1, STAT-3, and STAT-6 activities in THP-1 derived macrophages infected with two Trypanosoma cruzi strains
title_sort modulation of stat-1, stat-3, and stat-6 activities in thp-1 derived macrophages infected with two trypanosoma cruzi strains
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9643828/
https://www.ncbi.nlm.nih.gov/pubmed/36389843
http://dx.doi.org/10.3389/fimmu.2022.1038332
work_keys_str_mv AT oliveiramelissamartins modulationofstat1stat3andstat6activitiesinthp1derivedmacrophagesinfectedwithtwotrypanosomacruzistrains
AT bonturicamilaramalho modulationofstat1stat3andstat6activitiesinthp1derivedmacrophagesinfectedwithtwotrypanosomacruzistrains
AT salubrunoramos modulationofstat1stat3andstat6activitiesinthp1derivedmacrophagesinfectedwithtwotrypanosomacruzistrains
AT olivamarialuizavilela modulationofstat1stat3andstat6activitiesinthp1derivedmacrophagesinfectedwithtwotrypanosomacruzistrains
AT mortararenatoarruda modulationofstat1stat3andstat6activitiesinthp1derivedmacrophagesinfectedwithtwotrypanosomacruzistrains
AT orikazacristinamary modulationofstat1stat3andstat6activitiesinthp1derivedmacrophagesinfectedwithtwotrypanosomacruzistrains