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The N‐terminus of a Fusarium graminearum‐secreted protein enhances broad‐spectrum disease resistance in plants
Fusarium head blight is a destructive disease caused by Fusarium species. Little is known about the pathogenic molecular weapons of Fusarium graminearum. The gene encoding a small secreted protein, Fg02685, in F. graminearum was found to be upregulated during wheat head infection. Knockout mutation...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9644276/ https://www.ncbi.nlm.nih.gov/pubmed/35998056 http://dx.doi.org/10.1111/mpp.13262 |
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author | Xu, Qiang Hu, Su Jin, Minxia Xu, Yangjie Jiang, Qiantao Ma, Jian Zhang, Yazhou Qi, Pengfei Chen, Guoyue Jiang, Yunfeng Zheng, Youliang Wei, Yuming |
author_facet | Xu, Qiang Hu, Su Jin, Minxia Xu, Yangjie Jiang, Qiantao Ma, Jian Zhang, Yazhou Qi, Pengfei Chen, Guoyue Jiang, Yunfeng Zheng, Youliang Wei, Yuming |
author_sort | Xu, Qiang |
collection | PubMed |
description | Fusarium head blight is a destructive disease caused by Fusarium species. Little is known about the pathogenic molecular weapons of Fusarium graminearum. The gene encoding a small secreted protein, Fg02685, in F. graminearum was found to be upregulated during wheat head infection. Knockout mutation of Fg02685 reduced the growth and development of Fusarium in wheat spikes. Transient expression of Fg02685 or recombinant protein led to plant cell death in a BAK1‐ and SOBIR1‐independent system. Fg02685 was found to trigger plant basal immunity by increasing the deposition of callose, the accumulation of reactive oxygen species (ROS), and the expression of defence‐related genes. The Fg02685 signal peptide was required for the plant's apoplast accumulation and induces cell death, indicating Fg02685 is a novel conserved pathogen‐associated molecular pattern. Moreover, its homologues are widely distributed in oomycetes and fungal pathogens and induced cell death in tobacco. The conserved α‐helical motif at the N‐terminus was necessary for the induction of cell death. Moreover, a 32‐amino‐acid peptide, Fg02685 N‐terminus peptide 32 (FgNP32), was essential for the induction of oxidative burst, callose deposition, and mitogen‐activated protein kinase signal activation in plants. Prolonged exposure to FgNP32 enhanced the plant's resistance to Fusarium and Phytophthora. This study provides new approaches for an environment‐friendly control strategy for crop diseases by applying plant immune inducers to strengthen broad‐spectrum disease resistance in crops. |
format | Online Article Text |
id | pubmed-9644276 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96442762022-11-14 The N‐terminus of a Fusarium graminearum‐secreted protein enhances broad‐spectrum disease resistance in plants Xu, Qiang Hu, Su Jin, Minxia Xu, Yangjie Jiang, Qiantao Ma, Jian Zhang, Yazhou Qi, Pengfei Chen, Guoyue Jiang, Yunfeng Zheng, Youliang Wei, Yuming Mol Plant Pathol Original Articles Fusarium head blight is a destructive disease caused by Fusarium species. Little is known about the pathogenic molecular weapons of Fusarium graminearum. The gene encoding a small secreted protein, Fg02685, in F. graminearum was found to be upregulated during wheat head infection. Knockout mutation of Fg02685 reduced the growth and development of Fusarium in wheat spikes. Transient expression of Fg02685 or recombinant protein led to plant cell death in a BAK1‐ and SOBIR1‐independent system. Fg02685 was found to trigger plant basal immunity by increasing the deposition of callose, the accumulation of reactive oxygen species (ROS), and the expression of defence‐related genes. The Fg02685 signal peptide was required for the plant's apoplast accumulation and induces cell death, indicating Fg02685 is a novel conserved pathogen‐associated molecular pattern. Moreover, its homologues are widely distributed in oomycetes and fungal pathogens and induced cell death in tobacco. The conserved α‐helical motif at the N‐terminus was necessary for the induction of cell death. Moreover, a 32‐amino‐acid peptide, Fg02685 N‐terminus peptide 32 (FgNP32), was essential for the induction of oxidative burst, callose deposition, and mitogen‐activated protein kinase signal activation in plants. Prolonged exposure to FgNP32 enhanced the plant's resistance to Fusarium and Phytophthora. This study provides new approaches for an environment‐friendly control strategy for crop diseases by applying plant immune inducers to strengthen broad‐spectrum disease resistance in crops. John Wiley and Sons Inc. 2022-08-23 /pmc/articles/PMC9644276/ /pubmed/35998056 http://dx.doi.org/10.1111/mpp.13262 Text en © 2022 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Xu, Qiang Hu, Su Jin, Minxia Xu, Yangjie Jiang, Qiantao Ma, Jian Zhang, Yazhou Qi, Pengfei Chen, Guoyue Jiang, Yunfeng Zheng, Youliang Wei, Yuming The N‐terminus of a Fusarium graminearum‐secreted protein enhances broad‐spectrum disease resistance in plants |
title | The N‐terminus of a Fusarium graminearum‐secreted protein enhances broad‐spectrum disease resistance in plants |
title_full | The N‐terminus of a Fusarium graminearum‐secreted protein enhances broad‐spectrum disease resistance in plants |
title_fullStr | The N‐terminus of a Fusarium graminearum‐secreted protein enhances broad‐spectrum disease resistance in plants |
title_full_unstemmed | The N‐terminus of a Fusarium graminearum‐secreted protein enhances broad‐spectrum disease resistance in plants |
title_short | The N‐terminus of a Fusarium graminearum‐secreted protein enhances broad‐spectrum disease resistance in plants |
title_sort | n‐terminus of a fusarium graminearum‐secreted protein enhances broad‐spectrum disease resistance in plants |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9644276/ https://www.ncbi.nlm.nih.gov/pubmed/35998056 http://dx.doi.org/10.1111/mpp.13262 |
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