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The mixed liver and kidney transcriptome dataset of Darevskia valentini rock lizard

OBJECTIVES: This study is performed in the frame of a bigger study dedicated to genomics and transcriptomics of parthenogenesis in vertebrates. Among vertebrates, obligate parthenogenesis was first described in the lizards of the genus Darevskia. In this genus, all found parthenogenetic species orig...

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Autores principales: Ryakhovsky, Sergei S., Zhernakova, Daria V., Korchagin, Vitaly I., Vergun, Andrey A., Girnyk, Anastasiya E., Dikaya, Victoria A., Arakelyan, Marine S., Komissarov, Aleksey S., Ryskov, Alexey P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9644632/
https://www.ncbi.nlm.nih.gov/pubmed/36348468
http://dx.doi.org/10.1186/s13104-022-06228-4
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author Ryakhovsky, Sergei S.
Zhernakova, Daria V.
Korchagin, Vitaly I.
Vergun, Andrey A.
Girnyk, Anastasiya E.
Dikaya, Victoria A.
Arakelyan, Marine S.
Komissarov, Aleksey S.
Ryskov, Alexey P.
author_facet Ryakhovsky, Sergei S.
Zhernakova, Daria V.
Korchagin, Vitaly I.
Vergun, Andrey A.
Girnyk, Anastasiya E.
Dikaya, Victoria A.
Arakelyan, Marine S.
Komissarov, Aleksey S.
Ryskov, Alexey P.
author_sort Ryakhovsky, Sergei S.
collection PubMed
description OBJECTIVES: This study is performed in the frame of a bigger study dedicated to genomics and transcriptomics of parthenogenesis in vertebrates. Among vertebrates, obligate parthenogenesis was first described in the lizards of the genus Darevskia. In this genus, all found parthenogenetic species originated via interspecific hybridization. It remains unknown which genetic or genomic factors play a key role in the generation of parthenogenetic organisms. Comparative genomic and transcriptomic analysis of parthenogens and their parental species may elucidate this problem. Darevskia valentini is a paternal species for four (of seven) parthenogens of this genus, which we promote as a particularly important species for the generation of parthenogenetic forms. DATA DESCRIPTION: Total cellular RNA was isolated from kidney and liver tissues using the standard Trizol Tissue RNA Extraction protocol. Sequencing of transcriptome libraries prepared by random fragmentation of cDNA samples was performed on an Illumina HiSeq2500. Obtained raw sequences contained 117,6 million reads with the GC content of 47%. After preprocessing, raw data was assembled by Trinity and produced 491,482 contigs.
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spelling pubmed-96446322022-11-15 The mixed liver and kidney transcriptome dataset of Darevskia valentini rock lizard Ryakhovsky, Sergei S. Zhernakova, Daria V. Korchagin, Vitaly I. Vergun, Andrey A. Girnyk, Anastasiya E. Dikaya, Victoria A. Arakelyan, Marine S. Komissarov, Aleksey S. Ryskov, Alexey P. BMC Res Notes Data Note OBJECTIVES: This study is performed in the frame of a bigger study dedicated to genomics and transcriptomics of parthenogenesis in vertebrates. Among vertebrates, obligate parthenogenesis was first described in the lizards of the genus Darevskia. In this genus, all found parthenogenetic species originated via interspecific hybridization. It remains unknown which genetic or genomic factors play a key role in the generation of parthenogenetic organisms. Comparative genomic and transcriptomic analysis of parthenogens and their parental species may elucidate this problem. Darevskia valentini is a paternal species for four (of seven) parthenogens of this genus, which we promote as a particularly important species for the generation of parthenogenetic forms. DATA DESCRIPTION: Total cellular RNA was isolated from kidney and liver tissues using the standard Trizol Tissue RNA Extraction protocol. Sequencing of transcriptome libraries prepared by random fragmentation of cDNA samples was performed on an Illumina HiSeq2500. Obtained raw sequences contained 117,6 million reads with the GC content of 47%. After preprocessing, raw data was assembled by Trinity and produced 491,482 contigs. BioMed Central 2022-11-08 /pmc/articles/PMC9644632/ /pubmed/36348468 http://dx.doi.org/10.1186/s13104-022-06228-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Data Note
Ryakhovsky, Sergei S.
Zhernakova, Daria V.
Korchagin, Vitaly I.
Vergun, Andrey A.
Girnyk, Anastasiya E.
Dikaya, Victoria A.
Arakelyan, Marine S.
Komissarov, Aleksey S.
Ryskov, Alexey P.
The mixed liver and kidney transcriptome dataset of Darevskia valentini rock lizard
title The mixed liver and kidney transcriptome dataset of Darevskia valentini rock lizard
title_full The mixed liver and kidney transcriptome dataset of Darevskia valentini rock lizard
title_fullStr The mixed liver and kidney transcriptome dataset of Darevskia valentini rock lizard
title_full_unstemmed The mixed liver and kidney transcriptome dataset of Darevskia valentini rock lizard
title_short The mixed liver and kidney transcriptome dataset of Darevskia valentini rock lizard
title_sort mixed liver and kidney transcriptome dataset of darevskia valentini rock lizard
topic Data Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9644632/
https://www.ncbi.nlm.nih.gov/pubmed/36348468
http://dx.doi.org/10.1186/s13104-022-06228-4
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