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Integrating transcriptomics and metabolomics to analyze quinoa (Chenopodium quinoa Willd.) responses to drought stress and rewatering

The crop production of quinoa (Chenopodium quinoa Willd.), the only plant meeting basic human nutritional requirements, is affected by drought stress. To better understand the drought tolerance mechanism of quinoa, we screened the drought-tolerant quinoa genotype “Dianli 129” and studied the seedlin...

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Autores principales: Huan, Xiuju, Li, Li, Liu, Yongjiang, Kong, Zhiyou, Liu, Yeju, Wang, Qianchao, Liu, Junna, Zhang, Ping, Guo, Yirui, Qin, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9645111/
https://www.ncbi.nlm.nih.gov/pubmed/36388589
http://dx.doi.org/10.3389/fpls.2022.988861
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author Huan, Xiuju
Li, Li
Liu, Yongjiang
Kong, Zhiyou
Liu, Yeju
Wang, Qianchao
Liu, Junna
Zhang, Ping
Guo, Yirui
Qin, Peng
author_facet Huan, Xiuju
Li, Li
Liu, Yongjiang
Kong, Zhiyou
Liu, Yeju
Wang, Qianchao
Liu, Junna
Zhang, Ping
Guo, Yirui
Qin, Peng
author_sort Huan, Xiuju
collection PubMed
description The crop production of quinoa (Chenopodium quinoa Willd.), the only plant meeting basic human nutritional requirements, is affected by drought stress. To better understand the drought tolerance mechanism of quinoa, we screened the drought-tolerant quinoa genotype “Dianli 129” and studied the seedling leaves of the drought-tolerant quinoa genotype after drought and rewatering treatments using transcriptomics and targeted metabolomics. Drought-treatment, drought control, rewatering-treated, and rewatered control were named as DR, DC, RW, and RC, respectively. Among four comparison groups, DC vs. DR, RC vs. RW, RW vs. DR, and RC vs. DC, we identified 10,292, 2,307, 12,368, and 3 differentially expressed genes (DEGs), and 215, 192, 132, and 19 differentially expressed metabolites (DEMs), respectively. A total of 38,670 genes and 142 pathways were annotated. The results of transcriptome and metabolome association analysis showed that gene-LOC110713661 and gene-LOC110738152 may be the key genes for drought tolerance in quinoa. Some metabolites accumulated in quinoa leaves in response to drought stress, and the plants recovered after rewatering. DEGs and DEMs participate in starch and sucrose metabolism and flavonoid biosynthesis, which are vital for improving drought tolerance in quinoa. Drought tolerance of quinoa was correlated with gene expression differences, metabolite accumulation and good recovery after rewatering. These findings improve our understanding of drought and rewatering responses in quinoa and have implications for the breeding of new drought-tolerance varieties while providing a theoretical basis for drought-tolerance varieties identification.
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spelling pubmed-96451112022-11-15 Integrating transcriptomics and metabolomics to analyze quinoa (Chenopodium quinoa Willd.) responses to drought stress and rewatering Huan, Xiuju Li, Li Liu, Yongjiang Kong, Zhiyou Liu, Yeju Wang, Qianchao Liu, Junna Zhang, Ping Guo, Yirui Qin, Peng Front Plant Sci Plant Science The crop production of quinoa (Chenopodium quinoa Willd.), the only plant meeting basic human nutritional requirements, is affected by drought stress. To better understand the drought tolerance mechanism of quinoa, we screened the drought-tolerant quinoa genotype “Dianli 129” and studied the seedling leaves of the drought-tolerant quinoa genotype after drought and rewatering treatments using transcriptomics and targeted metabolomics. Drought-treatment, drought control, rewatering-treated, and rewatered control were named as DR, DC, RW, and RC, respectively. Among four comparison groups, DC vs. DR, RC vs. RW, RW vs. DR, and RC vs. DC, we identified 10,292, 2,307, 12,368, and 3 differentially expressed genes (DEGs), and 215, 192, 132, and 19 differentially expressed metabolites (DEMs), respectively. A total of 38,670 genes and 142 pathways were annotated. The results of transcriptome and metabolome association analysis showed that gene-LOC110713661 and gene-LOC110738152 may be the key genes for drought tolerance in quinoa. Some metabolites accumulated in quinoa leaves in response to drought stress, and the plants recovered after rewatering. DEGs and DEMs participate in starch and sucrose metabolism and flavonoid biosynthesis, which are vital for improving drought tolerance in quinoa. Drought tolerance of quinoa was correlated with gene expression differences, metabolite accumulation and good recovery after rewatering. These findings improve our understanding of drought and rewatering responses in quinoa and have implications for the breeding of new drought-tolerance varieties while providing a theoretical basis for drought-tolerance varieties identification. Frontiers Media S.A. 2022-10-26 /pmc/articles/PMC9645111/ /pubmed/36388589 http://dx.doi.org/10.3389/fpls.2022.988861 Text en Copyright © 2022 Huan, Li, Liu, Kong, Liu, Wang, Liu, Zhang, Guo and Qin https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Huan, Xiuju
Li, Li
Liu, Yongjiang
Kong, Zhiyou
Liu, Yeju
Wang, Qianchao
Liu, Junna
Zhang, Ping
Guo, Yirui
Qin, Peng
Integrating transcriptomics and metabolomics to analyze quinoa (Chenopodium quinoa Willd.) responses to drought stress and rewatering
title Integrating transcriptomics and metabolomics to analyze quinoa (Chenopodium quinoa Willd.) responses to drought stress and rewatering
title_full Integrating transcriptomics and metabolomics to analyze quinoa (Chenopodium quinoa Willd.) responses to drought stress and rewatering
title_fullStr Integrating transcriptomics and metabolomics to analyze quinoa (Chenopodium quinoa Willd.) responses to drought stress and rewatering
title_full_unstemmed Integrating transcriptomics and metabolomics to analyze quinoa (Chenopodium quinoa Willd.) responses to drought stress and rewatering
title_short Integrating transcriptomics and metabolomics to analyze quinoa (Chenopodium quinoa Willd.) responses to drought stress and rewatering
title_sort integrating transcriptomics and metabolomics to analyze quinoa (chenopodium quinoa willd.) responses to drought stress and rewatering
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9645111/
https://www.ncbi.nlm.nih.gov/pubmed/36388589
http://dx.doi.org/10.3389/fpls.2022.988861
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