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Theoretical analysis for the fluctuation in the electric parameters of the electroporated cells before and during the electrofusion

An electric pulse with a sufficient amplitude can lead to electroporation of intracellular organelles. Also, the electric field can lead to electrofusion of the neighboring cells. In this paper, a finite element mathematical model was used to simulate the distribution, radius, and density of the por...

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Autores principales: Sherif, Sameh, Ghallab, Yehya H., Ismail, Yehea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9646573/
https://www.ncbi.nlm.nih.gov/pubmed/36258107
http://dx.doi.org/10.1007/s11517-022-02683-0
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author Sherif, Sameh
Ghallab, Yehya H.
Ismail, Yehea
author_facet Sherif, Sameh
Ghallab, Yehya H.
Ismail, Yehea
author_sort Sherif, Sameh
collection PubMed
description An electric pulse with a sufficient amplitude can lead to electroporation of intracellular organelles. Also, the electric field can lead to electrofusion of the neighboring cells. In this paper, a finite element mathematical model was used to simulate the distribution, radius, and density of the pores. We simulated a mathematical model of the two neighbor cells to analyze the fluctuation in the electroporation parameters before the electrofusion under the ultra-shorted electric field pulse (i.e., impulse signal) for each cell separately and after the electrofusion under the ultra-shorted pulse. The analysis of the temporal and spatial distribution can lead to improving the mathematical models that are used to analyze both electroporation and electrofusion. The study combines the advantages of the nanosecond pulse to avoid the effect of the cell size on the electrofusion and the large-pore radius at the contact point between the cells. GRAPHICAL ABSTRACT: [Image: see text]
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spelling pubmed-96465732022-11-15 Theoretical analysis for the fluctuation in the electric parameters of the electroporated cells before and during the electrofusion Sherif, Sameh Ghallab, Yehya H. Ismail, Yehea Med Biol Eng Comput Original Article An electric pulse with a sufficient amplitude can lead to electroporation of intracellular organelles. Also, the electric field can lead to electrofusion of the neighboring cells. In this paper, a finite element mathematical model was used to simulate the distribution, radius, and density of the pores. We simulated a mathematical model of the two neighbor cells to analyze the fluctuation in the electroporation parameters before the electrofusion under the ultra-shorted electric field pulse (i.e., impulse signal) for each cell separately and after the electrofusion under the ultra-shorted pulse. The analysis of the temporal and spatial distribution can lead to improving the mathematical models that are used to analyze both electroporation and electrofusion. The study combines the advantages of the nanosecond pulse to avoid the effect of the cell size on the electrofusion and the large-pore radius at the contact point between the cells. GRAPHICAL ABSTRACT: [Image: see text] Springer Berlin Heidelberg 2022-10-19 2022 /pmc/articles/PMC9646573/ /pubmed/36258107 http://dx.doi.org/10.1007/s11517-022-02683-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Sherif, Sameh
Ghallab, Yehya H.
Ismail, Yehea
Theoretical analysis for the fluctuation in the electric parameters of the electroporated cells before and during the electrofusion
title Theoretical analysis for the fluctuation in the electric parameters of the electroporated cells before and during the electrofusion
title_full Theoretical analysis for the fluctuation in the electric parameters of the electroporated cells before and during the electrofusion
title_fullStr Theoretical analysis for the fluctuation in the electric parameters of the electroporated cells before and during the electrofusion
title_full_unstemmed Theoretical analysis for the fluctuation in the electric parameters of the electroporated cells before and during the electrofusion
title_short Theoretical analysis for the fluctuation in the electric parameters of the electroporated cells before and during the electrofusion
title_sort theoretical analysis for the fluctuation in the electric parameters of the electroporated cells before and during the electrofusion
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9646573/
https://www.ncbi.nlm.nih.gov/pubmed/36258107
http://dx.doi.org/10.1007/s11517-022-02683-0
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