Cargando…
In silico comparative genomic analysis unravels a new candidate protein arsenal specifically associated with Fusarium oxysporum f. sp. albedinis pathogenesis
Fusarium oxysporum f. sp albedinis (Foa) is a devastating fungus of date palms. To unravel the genetic characteristics associated with its pathogenesis, the two available genomes of Foa 133 and Foa 9 were compared with 49 genomes of 29 other pathogenic formae speciales belonging to Fusarium oxysporu...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9646873/ https://www.ncbi.nlm.nih.gov/pubmed/36351932 http://dx.doi.org/10.1038/s41598-022-21858-1 |
_version_ | 1784827262924750848 |
---|---|
author | Ayada, Hafida Dhioui, Boutayna Mazouz, Hamid El harrak, Abdelhay Jaiti, Fatima Ouhmidou, Bouchra Diouri, Mohammed Moumni, Mohieddine |
author_facet | Ayada, Hafida Dhioui, Boutayna Mazouz, Hamid El harrak, Abdelhay Jaiti, Fatima Ouhmidou, Bouchra Diouri, Mohammed Moumni, Mohieddine |
author_sort | Ayada, Hafida |
collection | PubMed |
description | Fusarium oxysporum f. sp albedinis (Foa) is a devastating fungus of date palms. To unravel the genetic characteristics associated with its pathogenesis, the two available genomes of Foa 133 and Foa 9 were compared with 49 genomes of 29 other pathogenic formae speciales belonging to Fusarium oxysporum species complex (FOSC). Foa 133 and Foa 9 have genomes of 56.23 Mb and 65.56 Mb with 17460 and 19514 putative coding genes. Of these genes, 30% lack functional annotation with no similarity to characterized proteins. The remaining genes were involved in pathways essential to the fungi's life and their adaptation. Foa secretome analysis revealed that both Foa strains possess an expanded number of secreted effectors (3003 in Foa 133 and 2418 in Foa 9). Those include effectors encoded by Foa unique genes that are involved in Foa penetration (Egh16-like family), host defense mechanisms suppression (lysM family) and pathogen protection (cysteine-rich protein family). The accessory protein SIX6, which induces plant cell death, was also predicted in Foa. Further analysis of secreted CAZymes revealed an arsenal of enzymes involved in plant cell wall degradation. This arsenal includes an exclusively Foa-specific CAZyme (GH5-7). Transcription factors and membrane transporters (MFS) involved in fungicide efflux have been predicted in Foa, in addition to a variety of secondary metabolites. These comprise mycotoxins as well as chrysogin, the latter provides Foa with resistance against adverse environmental conditions. Our results revealed new Foa proteins that could be targeted in future research in order to manage Bayoud disease. |
format | Online Article Text |
id | pubmed-9646873 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-96468732022-11-15 In silico comparative genomic analysis unravels a new candidate protein arsenal specifically associated with Fusarium oxysporum f. sp. albedinis pathogenesis Ayada, Hafida Dhioui, Boutayna Mazouz, Hamid El harrak, Abdelhay Jaiti, Fatima Ouhmidou, Bouchra Diouri, Mohammed Moumni, Mohieddine Sci Rep Article Fusarium oxysporum f. sp albedinis (Foa) is a devastating fungus of date palms. To unravel the genetic characteristics associated with its pathogenesis, the two available genomes of Foa 133 and Foa 9 were compared with 49 genomes of 29 other pathogenic formae speciales belonging to Fusarium oxysporum species complex (FOSC). Foa 133 and Foa 9 have genomes of 56.23 Mb and 65.56 Mb with 17460 and 19514 putative coding genes. Of these genes, 30% lack functional annotation with no similarity to characterized proteins. The remaining genes were involved in pathways essential to the fungi's life and their adaptation. Foa secretome analysis revealed that both Foa strains possess an expanded number of secreted effectors (3003 in Foa 133 and 2418 in Foa 9). Those include effectors encoded by Foa unique genes that are involved in Foa penetration (Egh16-like family), host defense mechanisms suppression (lysM family) and pathogen protection (cysteine-rich protein family). The accessory protein SIX6, which induces plant cell death, was also predicted in Foa. Further analysis of secreted CAZymes revealed an arsenal of enzymes involved in plant cell wall degradation. This arsenal includes an exclusively Foa-specific CAZyme (GH5-7). Transcription factors and membrane transporters (MFS) involved in fungicide efflux have been predicted in Foa, in addition to a variety of secondary metabolites. These comprise mycotoxins as well as chrysogin, the latter provides Foa with resistance against adverse environmental conditions. Our results revealed new Foa proteins that could be targeted in future research in order to manage Bayoud disease. Nature Publishing Group UK 2022-11-09 /pmc/articles/PMC9646873/ /pubmed/36351932 http://dx.doi.org/10.1038/s41598-022-21858-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Ayada, Hafida Dhioui, Boutayna Mazouz, Hamid El harrak, Abdelhay Jaiti, Fatima Ouhmidou, Bouchra Diouri, Mohammed Moumni, Mohieddine In silico comparative genomic analysis unravels a new candidate protein arsenal specifically associated with Fusarium oxysporum f. sp. albedinis pathogenesis |
title | In silico comparative genomic analysis unravels a new candidate protein arsenal specifically associated with Fusarium oxysporum f. sp. albedinis pathogenesis |
title_full | In silico comparative genomic analysis unravels a new candidate protein arsenal specifically associated with Fusarium oxysporum f. sp. albedinis pathogenesis |
title_fullStr | In silico comparative genomic analysis unravels a new candidate protein arsenal specifically associated with Fusarium oxysporum f. sp. albedinis pathogenesis |
title_full_unstemmed | In silico comparative genomic analysis unravels a new candidate protein arsenal specifically associated with Fusarium oxysporum f. sp. albedinis pathogenesis |
title_short | In silico comparative genomic analysis unravels a new candidate protein arsenal specifically associated with Fusarium oxysporum f. sp. albedinis pathogenesis |
title_sort | in silico comparative genomic analysis unravels a new candidate protein arsenal specifically associated with fusarium oxysporum f. sp. albedinis pathogenesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9646873/ https://www.ncbi.nlm.nih.gov/pubmed/36351932 http://dx.doi.org/10.1038/s41598-022-21858-1 |
work_keys_str_mv | AT ayadahafida insilicocomparativegenomicanalysisunravelsanewcandidateproteinarsenalspecificallyassociatedwithfusariumoxysporumfspalbedinispathogenesis AT dhiouiboutayna insilicocomparativegenomicanalysisunravelsanewcandidateproteinarsenalspecificallyassociatedwithfusariumoxysporumfspalbedinispathogenesis AT mazouzhamid insilicocomparativegenomicanalysisunravelsanewcandidateproteinarsenalspecificallyassociatedwithfusariumoxysporumfspalbedinispathogenesis AT elharrakabdelhay insilicocomparativegenomicanalysisunravelsanewcandidateproteinarsenalspecificallyassociatedwithfusariumoxysporumfspalbedinispathogenesis AT jaitifatima insilicocomparativegenomicanalysisunravelsanewcandidateproteinarsenalspecificallyassociatedwithfusariumoxysporumfspalbedinispathogenesis AT ouhmidoubouchra insilicocomparativegenomicanalysisunravelsanewcandidateproteinarsenalspecificallyassociatedwithfusariumoxysporumfspalbedinispathogenesis AT diourimohammed insilicocomparativegenomicanalysisunravelsanewcandidateproteinarsenalspecificallyassociatedwithfusariumoxysporumfspalbedinispathogenesis AT moumnimohieddine insilicocomparativegenomicanalysisunravelsanewcandidateproteinarsenalspecificallyassociatedwithfusariumoxysporumfspalbedinispathogenesis |