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Mutations in the SmAPRR2 transcription factor suppressing chlorophyll pigmentation in the eggplant fruit peel are key drivers of a diversified colour palette

Understanding the mechanisms by which chlorophylls are synthesized in the eggplant (Solanum melongena) fruit peel is of great relevance for eggplant breeding. A multi-parent advanced generation inter-cross (MAGIC) population and a germplasm collection have been screened for green pigmentation in the...

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Autores principales: Arrones, Andrea, Mangino, Giulio, Alonso, David, Plazas, Mariola, Prohens, Jaime, Portis, Ezio, Barchi, Lorenzo, Giuliano, Giovanni, Vilanova, Santiago, Gramazio, Pietro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9647125/
https://www.ncbi.nlm.nih.gov/pubmed/36388476
http://dx.doi.org/10.3389/fpls.2022.1025951
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author Arrones, Andrea
Mangino, Giulio
Alonso, David
Plazas, Mariola
Prohens, Jaime
Portis, Ezio
Barchi, Lorenzo
Giuliano, Giovanni
Vilanova, Santiago
Gramazio, Pietro
author_facet Arrones, Andrea
Mangino, Giulio
Alonso, David
Plazas, Mariola
Prohens, Jaime
Portis, Ezio
Barchi, Lorenzo
Giuliano, Giovanni
Vilanova, Santiago
Gramazio, Pietro
author_sort Arrones, Andrea
collection PubMed
description Understanding the mechanisms by which chlorophylls are synthesized in the eggplant (Solanum melongena) fruit peel is of great relevance for eggplant breeding. A multi-parent advanced generation inter-cross (MAGIC) population and a germplasm collection have been screened for green pigmentation in the fruit peel and used to identify candidate genes for this trait. A genome-wide association study (GWAS) performed with 420 MAGIC individuals revealed a major association on chromosome 8 close to a gene similar to APRR2. Two variants in SmAPRR2, predicted as having a high impact effect, were associated with the absence of fruit chlorophyll pigmentation in the MAGIC population, and a large deletion of 5.27 kb was found in two reference genomes of accessions without chlorophyll in the fruit peel. The validation of the candidate gene SmAPRR2 was performed by its sequencing in a set of MAGIC individuals and through its de novo assembly in 277 accessions from the G2P-SOL eggplant core collection. Two additional mutations in SmAPRR2 associated with the lack of chlorophyll were identified in the core collection set. The phylogenetic analysis of APRR2 reveals orthology within Solanaceae and suggests that specialization of APRR2-like genes occurred independently in Cucurbitaceae and Solanaceae. A strong geographical differentiation was observed in the frequency of predominant mutations in SmAPRR2, resulting in a lack of fruit chlorophyll pigmentation and suggesting that this phenotype may have arisen and been selected independently several times. This study represents the first identification of a major gene for fruit chlorophyll pigmentation in the eggplant fruit.
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spelling pubmed-96471252022-11-15 Mutations in the SmAPRR2 transcription factor suppressing chlorophyll pigmentation in the eggplant fruit peel are key drivers of a diversified colour palette Arrones, Andrea Mangino, Giulio Alonso, David Plazas, Mariola Prohens, Jaime Portis, Ezio Barchi, Lorenzo Giuliano, Giovanni Vilanova, Santiago Gramazio, Pietro Front Plant Sci Plant Science Understanding the mechanisms by which chlorophylls are synthesized in the eggplant (Solanum melongena) fruit peel is of great relevance for eggplant breeding. A multi-parent advanced generation inter-cross (MAGIC) population and a germplasm collection have been screened for green pigmentation in the fruit peel and used to identify candidate genes for this trait. A genome-wide association study (GWAS) performed with 420 MAGIC individuals revealed a major association on chromosome 8 close to a gene similar to APRR2. Two variants in SmAPRR2, predicted as having a high impact effect, were associated with the absence of fruit chlorophyll pigmentation in the MAGIC population, and a large deletion of 5.27 kb was found in two reference genomes of accessions without chlorophyll in the fruit peel. The validation of the candidate gene SmAPRR2 was performed by its sequencing in a set of MAGIC individuals and through its de novo assembly in 277 accessions from the G2P-SOL eggplant core collection. Two additional mutations in SmAPRR2 associated with the lack of chlorophyll were identified in the core collection set. The phylogenetic analysis of APRR2 reveals orthology within Solanaceae and suggests that specialization of APRR2-like genes occurred independently in Cucurbitaceae and Solanaceae. A strong geographical differentiation was observed in the frequency of predominant mutations in SmAPRR2, resulting in a lack of fruit chlorophyll pigmentation and suggesting that this phenotype may have arisen and been selected independently several times. This study represents the first identification of a major gene for fruit chlorophyll pigmentation in the eggplant fruit. Frontiers Media S.A. 2022-10-27 /pmc/articles/PMC9647125/ /pubmed/36388476 http://dx.doi.org/10.3389/fpls.2022.1025951 Text en Copyright © 2022 Arrones, Mangino, Alonso, Plazas, Prohens, Portis, Barchi, Giuliano, Vilanova and Gramazio https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Arrones, Andrea
Mangino, Giulio
Alonso, David
Plazas, Mariola
Prohens, Jaime
Portis, Ezio
Barchi, Lorenzo
Giuliano, Giovanni
Vilanova, Santiago
Gramazio, Pietro
Mutations in the SmAPRR2 transcription factor suppressing chlorophyll pigmentation in the eggplant fruit peel are key drivers of a diversified colour palette
title Mutations in the SmAPRR2 transcription factor suppressing chlorophyll pigmentation in the eggplant fruit peel are key drivers of a diversified colour palette
title_full Mutations in the SmAPRR2 transcription factor suppressing chlorophyll pigmentation in the eggplant fruit peel are key drivers of a diversified colour palette
title_fullStr Mutations in the SmAPRR2 transcription factor suppressing chlorophyll pigmentation in the eggplant fruit peel are key drivers of a diversified colour palette
title_full_unstemmed Mutations in the SmAPRR2 transcription factor suppressing chlorophyll pigmentation in the eggplant fruit peel are key drivers of a diversified colour palette
title_short Mutations in the SmAPRR2 transcription factor suppressing chlorophyll pigmentation in the eggplant fruit peel are key drivers of a diversified colour palette
title_sort mutations in the smaprr2 transcription factor suppressing chlorophyll pigmentation in the eggplant fruit peel are key drivers of a diversified colour palette
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9647125/
https://www.ncbi.nlm.nih.gov/pubmed/36388476
http://dx.doi.org/10.3389/fpls.2022.1025951
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