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A new combinatorial megaplasmid library assembly method designed to screen for minimal pathways by using SCRaMbLE
Human proteins expressed in yeast are common to enhance protein production while the expression of functional human pathways remain challenging. Here, we propose a simple and economical high-throughput gene assembly method to create a yeast megaplasmid library from human cDNA to screen for minimal h...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Caltech Library
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9647409/ https://www.ncbi.nlm.nih.gov/pubmed/36389121 http://dx.doi.org/10.17912/micropub.biology.000657 |