Lncrna FGD5-AS1 Aggravates Myocardial Ischemia-Reperfusion Injury by Sponging Mir-129-5p

BACKGROUND: LncRNA FGD5-AS1 regulates the pathogenesis of many human diseases. We aimed to elucidate the function of lncRNA FGD5-AS1 and the regulatory mechanism of lncRNA FGD5-AS1/miR-129-5p in myocardial ischemia-reperfusion (I/R) injury. METHODS: Myocardial I/R injury mice model and H/R treated H...

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Autores principales: Wei, Peng, Dong, Zhifeng, Lou, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9647623/
https://www.ncbi.nlm.nih.gov/pubmed/36415802
http://dx.doi.org/10.18502/ijph.v51i10.10986
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author Wei, Peng
Dong, Zhifeng
Lou, Ming
author_facet Wei, Peng
Dong, Zhifeng
Lou, Ming
author_sort Wei, Peng
collection PubMed
description BACKGROUND: LncRNA FGD5-AS1 regulates the pathogenesis of many human diseases. We aimed to elucidate the function of lncRNA FGD5-AS1 and the regulatory mechanism of lncRNA FGD5-AS1/miR-129-5p in myocardial ischemia-reperfusion (I/R) injury. METHODS: Myocardial I/R injury mice model and H/R treated H9c2 cells were established. RT-qPCR and Western blot analysis were used to detect the mRNA and protein expression. Cell viability was detected by MTT assay. Dual luciferase reporter assay was applied to confirm the relationship between lncRNA FGD5-AS1 and miR-129-5p. RESULTS: LncRNA FGD5-AS1 was upregulated in myocardial I/R injury mice models and H/R treated H9c2 cells. Functionally, knockdown of lncRNA FGD5-AS1 promoted cell viability and inhibited apoptosis in H/R treated H9c2 cells. In addition, lncRNA FGD5-AS1 directly targets miR-129-5p. Upregulation of lncRNA FGD5-AS1 weakened the protective effect of miR-129-5p on myocardial I/R injury. CONCLUSION: LncRNA FGD5-AS1 aggravates myocardial I/R injury by downregulating miR-129-5p.
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spelling pubmed-96476232022-11-21 Lncrna FGD5-AS1 Aggravates Myocardial Ischemia-Reperfusion Injury by Sponging Mir-129-5p Wei, Peng Dong, Zhifeng Lou, Ming Iran J Public Health Original Article BACKGROUND: LncRNA FGD5-AS1 regulates the pathogenesis of many human diseases. We aimed to elucidate the function of lncRNA FGD5-AS1 and the regulatory mechanism of lncRNA FGD5-AS1/miR-129-5p in myocardial ischemia-reperfusion (I/R) injury. METHODS: Myocardial I/R injury mice model and H/R treated H9c2 cells were established. RT-qPCR and Western blot analysis were used to detect the mRNA and protein expression. Cell viability was detected by MTT assay. Dual luciferase reporter assay was applied to confirm the relationship between lncRNA FGD5-AS1 and miR-129-5p. RESULTS: LncRNA FGD5-AS1 was upregulated in myocardial I/R injury mice models and H/R treated H9c2 cells. Functionally, knockdown of lncRNA FGD5-AS1 promoted cell viability and inhibited apoptosis in H/R treated H9c2 cells. In addition, lncRNA FGD5-AS1 directly targets miR-129-5p. Upregulation of lncRNA FGD5-AS1 weakened the protective effect of miR-129-5p on myocardial I/R injury. CONCLUSION: LncRNA FGD5-AS1 aggravates myocardial I/R injury by downregulating miR-129-5p. Tehran University of Medical Sciences 2022-10 /pmc/articles/PMC9647623/ /pubmed/36415802 http://dx.doi.org/10.18502/ijph.v51i10.10986 Text en Copyright © 2022 Wei et al. Published by Tehran University of Medical Sciences https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (https://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Article
Wei, Peng
Dong, Zhifeng
Lou, Ming
Lncrna FGD5-AS1 Aggravates Myocardial Ischemia-Reperfusion Injury by Sponging Mir-129-5p
title Lncrna FGD5-AS1 Aggravates Myocardial Ischemia-Reperfusion Injury by Sponging Mir-129-5p
title_full Lncrna FGD5-AS1 Aggravates Myocardial Ischemia-Reperfusion Injury by Sponging Mir-129-5p
title_fullStr Lncrna FGD5-AS1 Aggravates Myocardial Ischemia-Reperfusion Injury by Sponging Mir-129-5p
title_full_unstemmed Lncrna FGD5-AS1 Aggravates Myocardial Ischemia-Reperfusion Injury by Sponging Mir-129-5p
title_short Lncrna FGD5-AS1 Aggravates Myocardial Ischemia-Reperfusion Injury by Sponging Mir-129-5p
title_sort lncrna fgd5-as1 aggravates myocardial ischemia-reperfusion injury by sponging mir-129-5p
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9647623/
https://www.ncbi.nlm.nih.gov/pubmed/36415802
http://dx.doi.org/10.18502/ijph.v51i10.10986
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AT louming lncrnafgd5as1aggravatesmyocardialischemiareperfusioninjurybyspongingmir1295p

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