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The effects of thymoquinone on steroid-induced femoral head osteonecrosis: An experimental study in rats

OBJECTIVES: In this study, we aimed to evaluate the antioxidant, anti-apoptotic, osteoblastic and hypolipidemic effects of thymoquinone (TQ) treatment on the steroid-induced osteonecrosis of femoral head (ONFH) model in rats. MATERIALS AND METHODS: A total of 24 rats were randomly divided into four...

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Autores principales: Dasci, Mustafa Fatih, Yaprak Sarac, Elif, Gok Yurttas, Asiye, Atci, Tolgahan, Uslu, Muhammed, Acar, Abdurrahman, Gulec, Mehmet Akif, Alagoz, Ender
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bayçınar Medical Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9647667/
https://www.ncbi.nlm.nih.gov/pubmed/36345183
http://dx.doi.org/10.52312/jdrs.2022.752
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author Dasci, Mustafa Fatih
Yaprak Sarac, Elif
Gok Yurttas, Asiye
Atci, Tolgahan
Uslu, Muhammed
Acar, Abdurrahman
Gulec, Mehmet Akif
Alagoz, Ender
author_facet Dasci, Mustafa Fatih
Yaprak Sarac, Elif
Gok Yurttas, Asiye
Atci, Tolgahan
Uslu, Muhammed
Acar, Abdurrahman
Gulec, Mehmet Akif
Alagoz, Ender
author_sort Dasci, Mustafa Fatih
collection PubMed
description OBJECTIVES: In this study, we aimed to evaluate the antioxidant, anti-apoptotic, osteoblastic and hypolipidemic effects of thymoquinone (TQ) treatment on the steroid-induced osteonecrosis of femoral head (ONFH) model in rats. MATERIALS AND METHODS: A total of 24 rats were randomly divided into four groups: the control group administered saline; the TQ group administered 10 mg/kg/day TQ orally; lipopolysaccharide/methylprednisolone (LPS/MPS) group administered 20 µg/kg intraperitoneally LPS and 40 mg/kg intramuscularly MPS to establish ONFH model; and the LPS/ MPS+TQ group administered both LPS/MPS and, then, TQ once daily for four weeks. All rats were sacrificed after intracardiac blood collection and their right femurs were removed. RESULTS: Micro-computed tomography showed a higher bone mineral density and lower porosity, Tr. Sp and Tr. Sep data were detected in the LPS/MPS+TQ group. In histopathology, osteonecrosis increased significantly in the LPS/MPS group and osteonecrosis decreased in the LPS/MPS+TQ group compared to the LPS/MPS group (p=0.0077). Histomorphometric examination revealed that the percentage of BV/TV in the LPS/MPS group was significantly lower compared to control and other groups (p<0.01 and p<0.05, respectively), while it reached normal rates in the LPS/MPS+TQ group. Immunohistochemically, antioxidant, anti-apoptotic, and angiogenesis indicators (8-hydroxy-20- deoxyguanosine [8-OHdG], malondialdehyde [MDA], B-cell lymphoma [Bcl-2], caspase-3, vascular endothelial growth factor [VEGF]) were significantly improved in tissue and serum with TQ. Furthermore, TQ significantly reduced low- and high-density lipoprotein cholesterol ratio and carboxy-terminal type 1 collagen crosslink (CTX) in serum. CONCLUSION: Vascular and hematopoietic cell damages that occur due to steroid-induced deoxyribonucleic acid (DNA) oxidative and lipid peroxidative damages in an ONFH model can be successfully ameliorated by TQ administration. This antioxidant and anti-apoptotic effects of TQ may be a promising treatment option for early stage of osteonecrosis.
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spelling pubmed-96476672022-11-21 The effects of thymoquinone on steroid-induced femoral head osteonecrosis: An experimental study in rats Dasci, Mustafa Fatih Yaprak Sarac, Elif Gok Yurttas, Asiye Atci, Tolgahan Uslu, Muhammed Acar, Abdurrahman Gulec, Mehmet Akif Alagoz, Ender Jt Dis Relat Surg Original Article OBJECTIVES: In this study, we aimed to evaluate the antioxidant, anti-apoptotic, osteoblastic and hypolipidemic effects of thymoquinone (TQ) treatment on the steroid-induced osteonecrosis of femoral head (ONFH) model in rats. MATERIALS AND METHODS: A total of 24 rats were randomly divided into four groups: the control group administered saline; the TQ group administered 10 mg/kg/day TQ orally; lipopolysaccharide/methylprednisolone (LPS/MPS) group administered 20 µg/kg intraperitoneally LPS and 40 mg/kg intramuscularly MPS to establish ONFH model; and the LPS/ MPS+TQ group administered both LPS/MPS and, then, TQ once daily for four weeks. All rats were sacrificed after intracardiac blood collection and their right femurs were removed. RESULTS: Micro-computed tomography showed a higher bone mineral density and lower porosity, Tr. Sp and Tr. Sep data were detected in the LPS/MPS+TQ group. In histopathology, osteonecrosis increased significantly in the LPS/MPS group and osteonecrosis decreased in the LPS/MPS+TQ group compared to the LPS/MPS group (p=0.0077). Histomorphometric examination revealed that the percentage of BV/TV in the LPS/MPS group was significantly lower compared to control and other groups (p<0.01 and p<0.05, respectively), while it reached normal rates in the LPS/MPS+TQ group. Immunohistochemically, antioxidant, anti-apoptotic, and angiogenesis indicators (8-hydroxy-20- deoxyguanosine [8-OHdG], malondialdehyde [MDA], B-cell lymphoma [Bcl-2], caspase-3, vascular endothelial growth factor [VEGF]) were significantly improved in tissue and serum with TQ. Furthermore, TQ significantly reduced low- and high-density lipoprotein cholesterol ratio and carboxy-terminal type 1 collagen crosslink (CTX) in serum. CONCLUSION: Vascular and hematopoietic cell damages that occur due to steroid-induced deoxyribonucleic acid (DNA) oxidative and lipid peroxidative damages in an ONFH model can be successfully ameliorated by TQ administration. This antioxidant and anti-apoptotic effects of TQ may be a promising treatment option for early stage of osteonecrosis. Bayçınar Medical Publishing 2022-10-21 /pmc/articles/PMC9647667/ /pubmed/36345183 http://dx.doi.org/10.52312/jdrs.2022.752 Text en Copyright © 2022, Turkish Joint Diseases Foundation https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Article
Dasci, Mustafa Fatih
Yaprak Sarac, Elif
Gok Yurttas, Asiye
Atci, Tolgahan
Uslu, Muhammed
Acar, Abdurrahman
Gulec, Mehmet Akif
Alagoz, Ender
The effects of thymoquinone on steroid-induced femoral head osteonecrosis: An experimental study in rats
title The effects of thymoquinone on steroid-induced femoral head osteonecrosis: An experimental study in rats
title_full The effects of thymoquinone on steroid-induced femoral head osteonecrosis: An experimental study in rats
title_fullStr The effects of thymoquinone on steroid-induced femoral head osteonecrosis: An experimental study in rats
title_full_unstemmed The effects of thymoquinone on steroid-induced femoral head osteonecrosis: An experimental study in rats
title_short The effects of thymoquinone on steroid-induced femoral head osteonecrosis: An experimental study in rats
title_sort effects of thymoquinone on steroid-induced femoral head osteonecrosis: an experimental study in rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9647667/
https://www.ncbi.nlm.nih.gov/pubmed/36345183
http://dx.doi.org/10.52312/jdrs.2022.752
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