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Modified Silver Impregnation Method for Basal Membranes in Renal Biopsies
Silver impregnation methods are essential in biopsy interpretation in nephropathology with regard to visualizing the basal lamina and its associated changes. The most widely used methods, mainly Jones methenamine impregnation, are time-consuming in their protocols and require multiple microscopy con...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cureus
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648111/ https://www.ncbi.nlm.nih.gov/pubmed/36397906 http://dx.doi.org/10.7759/cureus.30171 |
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author | Popov, Hristo Stoyanov, George S Ghenev, Peter |
author_facet | Popov, Hristo Stoyanov, George S Ghenev, Peter |
author_sort | Popov, Hristo |
collection | PubMed |
description | Silver impregnation methods are essential in biopsy interpretation in nephropathology with regard to visualizing the basal lamina and its associated changes. The most widely used methods, mainly Jones methenamine impregnation, are time-consuming in their protocols and require multiple microscopy control points. In this report, we propose an alternative, modified method for silver impregnation with methenamine solution with a significantly shorter protocol time and good staining quality, allowing for proper interpretation of basal lamina changes in the glomeruli and blood vessels. Furthermore, unlike some other modified techniques, our proposed protocol does not include microwaving of the solutions but rather a thermostat is used, thereby reducing fire hazards. Implementing the protocol in our everyday practice has reduced sample processing time while not negatively impacting biopsy interpretation. |
format | Online Article Text |
id | pubmed-9648111 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Cureus |
record_format | MEDLINE/PubMed |
spelling | pubmed-96481112022-11-16 Modified Silver Impregnation Method for Basal Membranes in Renal Biopsies Popov, Hristo Stoyanov, George S Ghenev, Peter Cureus Pathology Silver impregnation methods are essential in biopsy interpretation in nephropathology with regard to visualizing the basal lamina and its associated changes. The most widely used methods, mainly Jones methenamine impregnation, are time-consuming in their protocols and require multiple microscopy control points. In this report, we propose an alternative, modified method for silver impregnation with methenamine solution with a significantly shorter protocol time and good staining quality, allowing for proper interpretation of basal lamina changes in the glomeruli and blood vessels. Furthermore, unlike some other modified techniques, our proposed protocol does not include microwaving of the solutions but rather a thermostat is used, thereby reducing fire hazards. Implementing the protocol in our everyday practice has reduced sample processing time while not negatively impacting biopsy interpretation. Cureus 2022-10-11 /pmc/articles/PMC9648111/ /pubmed/36397906 http://dx.doi.org/10.7759/cureus.30171 Text en Copyright © 2022, Popov et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Pathology Popov, Hristo Stoyanov, George S Ghenev, Peter Modified Silver Impregnation Method for Basal Membranes in Renal Biopsies |
title | Modified Silver Impregnation Method for Basal Membranes in Renal Biopsies |
title_full | Modified Silver Impregnation Method for Basal Membranes in Renal Biopsies |
title_fullStr | Modified Silver Impregnation Method for Basal Membranes in Renal Biopsies |
title_full_unstemmed | Modified Silver Impregnation Method for Basal Membranes in Renal Biopsies |
title_short | Modified Silver Impregnation Method for Basal Membranes in Renal Biopsies |
title_sort | modified silver impregnation method for basal membranes in renal biopsies |
topic | Pathology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648111/ https://www.ncbi.nlm.nih.gov/pubmed/36397906 http://dx.doi.org/10.7759/cureus.30171 |
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