Development of a New Solid-Phase Extraction Base Method for Free Saccharide Content Estimation of Meningococcal Conjugate Vaccines

[Image: see text] GlaxoSmithKline (GSK) is currently developing a fully liquid presentation to ease the administration of the licensed quadrivalent conjugate vaccine (Menveo) against meningococcal serogroup A, C, W, and Y (MenACWY) infections. Herein, we report a new method for determining the free...

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Autores principales: De Ricco, Riccardo, Rech, Francesca, Onnis, Valeria, Coccone, Salvatore Sanna, Scalia, Giusy, Marcozzi, Cristina, Gavini, Massimiliano, Beni, Sara, Giannini, Sara, Nompari, Luca, Parlati, Chiara, Magagnoli, Claudia, Cianetti, Simona, Berti, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648139/
https://www.ncbi.nlm.nih.gov/pubmed/36385865
http://dx.doi.org/10.1021/acsomega.2c04013
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author De Ricco, Riccardo
Rech, Francesca
Onnis, Valeria
Coccone, Salvatore Sanna
Scalia, Giusy
Marcozzi, Cristina
Gavini, Massimiliano
Beni, Sara
Giannini, Sara
Nompari, Luca
Parlati, Chiara
Magagnoli, Claudia
Cianetti, Simona
Berti, Francesco
author_facet De Ricco, Riccardo
Rech, Francesca
Onnis, Valeria
Coccone, Salvatore Sanna
Scalia, Giusy
Marcozzi, Cristina
Gavini, Massimiliano
Beni, Sara
Giannini, Sara
Nompari, Luca
Parlati, Chiara
Magagnoli, Claudia
Cianetti, Simona
Berti, Francesco
author_sort De Ricco, Riccardo
collection PubMed
description [Image: see text] GlaxoSmithKline (GSK) is currently developing a fully liquid presentation to ease the administration of the licensed quadrivalent conjugate vaccine (Menveo) against meningococcal serogroup A, C, W, and Y (MenACWY) infections. Herein, we report a new method for determining the free saccharide (FS) content of CRM(197)-MenACWY conjugated antigens, with the aim of improving accuracy and reproducibility. Mathematical models have been used to support technical knowledge in reducing the need for experimental development. This results in an improved, faster, and platform-based technique for FS separation with one single pretreatment applicable to all antigens of the multivalent meningococcal vaccine.
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spelling pubmed-96481392022-11-15 Development of a New Solid-Phase Extraction Base Method for Free Saccharide Content Estimation of Meningococcal Conjugate Vaccines De Ricco, Riccardo Rech, Francesca Onnis, Valeria Coccone, Salvatore Sanna Scalia, Giusy Marcozzi, Cristina Gavini, Massimiliano Beni, Sara Giannini, Sara Nompari, Luca Parlati, Chiara Magagnoli, Claudia Cianetti, Simona Berti, Francesco ACS Omega [Image: see text] GlaxoSmithKline (GSK) is currently developing a fully liquid presentation to ease the administration of the licensed quadrivalent conjugate vaccine (Menveo) against meningococcal serogroup A, C, W, and Y (MenACWY) infections. Herein, we report a new method for determining the free saccharide (FS) content of CRM(197)-MenACWY conjugated antigens, with the aim of improving accuracy and reproducibility. Mathematical models have been used to support technical knowledge in reducing the need for experimental development. This results in an improved, faster, and platform-based technique for FS separation with one single pretreatment applicable to all antigens of the multivalent meningococcal vaccine. American Chemical Society 2022-10-27 /pmc/articles/PMC9648139/ /pubmed/36385865 http://dx.doi.org/10.1021/acsomega.2c04013 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle De Ricco, Riccardo
Rech, Francesca
Onnis, Valeria
Coccone, Salvatore Sanna
Scalia, Giusy
Marcozzi, Cristina
Gavini, Massimiliano
Beni, Sara
Giannini, Sara
Nompari, Luca
Parlati, Chiara
Magagnoli, Claudia
Cianetti, Simona
Berti, Francesco
Development of a New Solid-Phase Extraction Base Method for Free Saccharide Content Estimation of Meningococcal Conjugate Vaccines
title Development of a New Solid-Phase Extraction Base Method for Free Saccharide Content Estimation of Meningococcal Conjugate Vaccines
title_full Development of a New Solid-Phase Extraction Base Method for Free Saccharide Content Estimation of Meningococcal Conjugate Vaccines
title_fullStr Development of a New Solid-Phase Extraction Base Method for Free Saccharide Content Estimation of Meningococcal Conjugate Vaccines
title_full_unstemmed Development of a New Solid-Phase Extraction Base Method for Free Saccharide Content Estimation of Meningococcal Conjugate Vaccines
title_short Development of a New Solid-Phase Extraction Base Method for Free Saccharide Content Estimation of Meningococcal Conjugate Vaccines
title_sort development of a new solid-phase extraction base method for free saccharide content estimation of meningococcal conjugate vaccines
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648139/
https://www.ncbi.nlm.nih.gov/pubmed/36385865
http://dx.doi.org/10.1021/acsomega.2c04013
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