Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly

SARS-CoV-2 is a lipid-enveloped Betacoronavirus and cause of the Covid-19 pandemic. To study the three-dimensional architecture of the virus, we perform electron cryotomography (cryo-ET) on SARS-Cov-2 virions and three variants revealing particles of regular cylindrical morphology. The ribonucleopro...

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Autores principales: Calder, Lesley J., Calcraft, Thomas, Hussain, Saira, Harvey, Ruth, Rosenthal, Peter B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648435/
https://www.ncbi.nlm.nih.gov/pubmed/36357779
http://dx.doi.org/10.1038/s42003-022-04183-1
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author Calder, Lesley J.
Calcraft, Thomas
Hussain, Saira
Harvey, Ruth
Rosenthal, Peter B.
author_facet Calder, Lesley J.
Calcraft, Thomas
Hussain, Saira
Harvey, Ruth
Rosenthal, Peter B.
author_sort Calder, Lesley J.
collection PubMed
description SARS-CoV-2 is a lipid-enveloped Betacoronavirus and cause of the Covid-19 pandemic. To study the three-dimensional architecture of the virus, we perform electron cryotomography (cryo-ET) on SARS-Cov-2 virions and three variants revealing particles of regular cylindrical morphology. The ribonucleoprotein particles packaging the genome in the virion interior form a dense, double layer assembly with a cylindrical shape related to the overall particle morphology. This organisation suggests structural interactions important to virus assembly.
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spelling pubmed-96484352022-11-14 Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly Calder, Lesley J. Calcraft, Thomas Hussain, Saira Harvey, Ruth Rosenthal, Peter B. Commun Biol Article SARS-CoV-2 is a lipid-enveloped Betacoronavirus and cause of the Covid-19 pandemic. To study the three-dimensional architecture of the virus, we perform electron cryotomography (cryo-ET) on SARS-Cov-2 virions and three variants revealing particles of regular cylindrical morphology. The ribonucleoprotein particles packaging the genome in the virion interior form a dense, double layer assembly with a cylindrical shape related to the overall particle morphology. This organisation suggests structural interactions important to virus assembly. Nature Publishing Group UK 2022-11-10 /pmc/articles/PMC9648435/ /pubmed/36357779 http://dx.doi.org/10.1038/s42003-022-04183-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Calder, Lesley J.
Calcraft, Thomas
Hussain, Saira
Harvey, Ruth
Rosenthal, Peter B.
Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly
title Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly
title_full Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly
title_fullStr Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly
title_full_unstemmed Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly
title_short Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly
title_sort electron cryotomography of sars-cov-2 virions reveals cylinder-shaped particles with a double layer rnp assembly
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648435/
https://www.ncbi.nlm.nih.gov/pubmed/36357779
http://dx.doi.org/10.1038/s42003-022-04183-1
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