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Characteristics of gut microbiota in patients with primary Sjögren’s syndrome in Northern China

This study analyzes and compares the structure and diversity of gut microbiota in patients with primary Sjögren’s syndrome (pSS) in Northern China to healthy individuals to identify clinical features associated with dysbiosis. We included 60 Chinese pSS patients and 50 age- and gender-matched health...

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Detalles Bibliográficos
Autores principales: Li, Yuyuan, Li, Zhi, Sun, Wenying, Wang, Meiling, Li, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648750/
https://www.ncbi.nlm.nih.gov/pubmed/36355832
http://dx.doi.org/10.1371/journal.pone.0277270
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author Li, Yuyuan
Li, Zhi
Sun, Wenying
Wang, Meiling
Li, Ming
author_facet Li, Yuyuan
Li, Zhi
Sun, Wenying
Wang, Meiling
Li, Ming
author_sort Li, Yuyuan
collection PubMed
description This study analyzes and compares the structure and diversity of gut microbiota in patients with primary Sjögren’s syndrome (pSS) in Northern China to healthy individuals to identify clinical features associated with dysbiosis. We included 60 Chinese pSS patients and 50 age- and gender-matched healthy controls. DNA was extracted from stool samples and subjected to 16S ribosomal RNA gene analysis (V3-V4) for intestinal dysbiosis. In addition, patients were examined for laboratory and serological pSS features. A Spearman’s correlation analysis was performed to assess correlations between individual bacteria taxa and clinical characteristics. The alpha-diversity (Chao1 and Shannon Index) and beta-diversity (unweighted UniFrac distances) of the gut microbiota differed significantly between pSS patients and healthy controls. Further analysis showed that several gut opportunistic pathogens (Bacteroides, Megamonas, and Veillonella) were significantly more abundant in pSS patients and positively correlated with their clinical indicators. In contrast, some probiotic genera (Collinsella, unidentified_Ruminococcaceae, Romboutsia, and Dorea) were significantly decreased in pSS patients and negatively correlated with their clinical indicators. Therefore, pSS patients in Northern China showed a dysbiotic intestinal microbiome enriched for potentially pathogenic genera that might be associated with autoimmune disease.
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spelling pubmed-96487502022-11-15 Characteristics of gut microbiota in patients with primary Sjögren’s syndrome in Northern China Li, Yuyuan Li, Zhi Sun, Wenying Wang, Meiling Li, Ming PLoS One Research Article This study analyzes and compares the structure and diversity of gut microbiota in patients with primary Sjögren’s syndrome (pSS) in Northern China to healthy individuals to identify clinical features associated with dysbiosis. We included 60 Chinese pSS patients and 50 age- and gender-matched healthy controls. DNA was extracted from stool samples and subjected to 16S ribosomal RNA gene analysis (V3-V4) for intestinal dysbiosis. In addition, patients were examined for laboratory and serological pSS features. A Spearman’s correlation analysis was performed to assess correlations between individual bacteria taxa and clinical characteristics. The alpha-diversity (Chao1 and Shannon Index) and beta-diversity (unweighted UniFrac distances) of the gut microbiota differed significantly between pSS patients and healthy controls. Further analysis showed that several gut opportunistic pathogens (Bacteroides, Megamonas, and Veillonella) were significantly more abundant in pSS patients and positively correlated with their clinical indicators. In contrast, some probiotic genera (Collinsella, unidentified_Ruminococcaceae, Romboutsia, and Dorea) were significantly decreased in pSS patients and negatively correlated with their clinical indicators. Therefore, pSS patients in Northern China showed a dysbiotic intestinal microbiome enriched for potentially pathogenic genera that might be associated with autoimmune disease. Public Library of Science 2022-11-10 /pmc/articles/PMC9648750/ /pubmed/36355832 http://dx.doi.org/10.1371/journal.pone.0277270 Text en © 2022 Li et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Li, Yuyuan
Li, Zhi
Sun, Wenying
Wang, Meiling
Li, Ming
Characteristics of gut microbiota in patients with primary Sjögren’s syndrome in Northern China
title Characteristics of gut microbiota in patients with primary Sjögren’s syndrome in Northern China
title_full Characteristics of gut microbiota in patients with primary Sjögren’s syndrome in Northern China
title_fullStr Characteristics of gut microbiota in patients with primary Sjögren’s syndrome in Northern China
title_full_unstemmed Characteristics of gut microbiota in patients with primary Sjögren’s syndrome in Northern China
title_short Characteristics of gut microbiota in patients with primary Sjögren’s syndrome in Northern China
title_sort characteristics of gut microbiota in patients with primary sjögren’s syndrome in northern china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648750/
https://www.ncbi.nlm.nih.gov/pubmed/36355832
http://dx.doi.org/10.1371/journal.pone.0277270
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