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Characteristics of gut microbiota in patients with primary Sjögren’s syndrome in Northern China
This study analyzes and compares the structure and diversity of gut microbiota in patients with primary Sjögren’s syndrome (pSS) in Northern China to healthy individuals to identify clinical features associated with dysbiosis. We included 60 Chinese pSS patients and 50 age- and gender-matched health...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648750/ https://www.ncbi.nlm.nih.gov/pubmed/36355832 http://dx.doi.org/10.1371/journal.pone.0277270 |
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author | Li, Yuyuan Li, Zhi Sun, Wenying Wang, Meiling Li, Ming |
author_facet | Li, Yuyuan Li, Zhi Sun, Wenying Wang, Meiling Li, Ming |
author_sort | Li, Yuyuan |
collection | PubMed |
description | This study analyzes and compares the structure and diversity of gut microbiota in patients with primary Sjögren’s syndrome (pSS) in Northern China to healthy individuals to identify clinical features associated with dysbiosis. We included 60 Chinese pSS patients and 50 age- and gender-matched healthy controls. DNA was extracted from stool samples and subjected to 16S ribosomal RNA gene analysis (V3-V4) for intestinal dysbiosis. In addition, patients were examined for laboratory and serological pSS features. A Spearman’s correlation analysis was performed to assess correlations between individual bacteria taxa and clinical characteristics. The alpha-diversity (Chao1 and Shannon Index) and beta-diversity (unweighted UniFrac distances) of the gut microbiota differed significantly between pSS patients and healthy controls. Further analysis showed that several gut opportunistic pathogens (Bacteroides, Megamonas, and Veillonella) were significantly more abundant in pSS patients and positively correlated with their clinical indicators. In contrast, some probiotic genera (Collinsella, unidentified_Ruminococcaceae, Romboutsia, and Dorea) were significantly decreased in pSS patients and negatively correlated with their clinical indicators. Therefore, pSS patients in Northern China showed a dysbiotic intestinal microbiome enriched for potentially pathogenic genera that might be associated with autoimmune disease. |
format | Online Article Text |
id | pubmed-9648750 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-96487502022-11-15 Characteristics of gut microbiota in patients with primary Sjögren’s syndrome in Northern China Li, Yuyuan Li, Zhi Sun, Wenying Wang, Meiling Li, Ming PLoS One Research Article This study analyzes and compares the structure and diversity of gut microbiota in patients with primary Sjögren’s syndrome (pSS) in Northern China to healthy individuals to identify clinical features associated with dysbiosis. We included 60 Chinese pSS patients and 50 age- and gender-matched healthy controls. DNA was extracted from stool samples and subjected to 16S ribosomal RNA gene analysis (V3-V4) for intestinal dysbiosis. In addition, patients were examined for laboratory and serological pSS features. A Spearman’s correlation analysis was performed to assess correlations between individual bacteria taxa and clinical characteristics. The alpha-diversity (Chao1 and Shannon Index) and beta-diversity (unweighted UniFrac distances) of the gut microbiota differed significantly between pSS patients and healthy controls. Further analysis showed that several gut opportunistic pathogens (Bacteroides, Megamonas, and Veillonella) were significantly more abundant in pSS patients and positively correlated with their clinical indicators. In contrast, some probiotic genera (Collinsella, unidentified_Ruminococcaceae, Romboutsia, and Dorea) were significantly decreased in pSS patients and negatively correlated with their clinical indicators. Therefore, pSS patients in Northern China showed a dysbiotic intestinal microbiome enriched for potentially pathogenic genera that might be associated with autoimmune disease. Public Library of Science 2022-11-10 /pmc/articles/PMC9648750/ /pubmed/36355832 http://dx.doi.org/10.1371/journal.pone.0277270 Text en © 2022 Li et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Li, Yuyuan Li, Zhi Sun, Wenying Wang, Meiling Li, Ming Characteristics of gut microbiota in patients with primary Sjögren’s syndrome in Northern China |
title | Characteristics of gut microbiota in patients with primary Sjögren’s syndrome in Northern China |
title_full | Characteristics of gut microbiota in patients with primary Sjögren’s syndrome in Northern China |
title_fullStr | Characteristics of gut microbiota in patients with primary Sjögren’s syndrome in Northern China |
title_full_unstemmed | Characteristics of gut microbiota in patients with primary Sjögren’s syndrome in Northern China |
title_short | Characteristics of gut microbiota in patients with primary Sjögren’s syndrome in Northern China |
title_sort | characteristics of gut microbiota in patients with primary sjögren’s syndrome in northern china |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648750/ https://www.ncbi.nlm.nih.gov/pubmed/36355832 http://dx.doi.org/10.1371/journal.pone.0277270 |
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