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Gelatin coating enhances therapeutic cell adhesion to the infarcted myocardium via ECM binding

Acute myocardial infarction (AMI) results in weakening of the heart muscle and an increased risk for chronic heart failure. Therapeutic stem cells have been shown to reduce inflammatory signaling and scar tissue expansion, despite most of these studies being limited by poor retention of cells. Gelat...

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Autores principales: Davis, Kara A., Gottipatti, Anuhya, Peng, Hsuan, Donahue, Renee, Chelvarajan, Lakshman, Cahall, Calvin, Tripathi, Himi, Al-Darraji, Ahmed, Ye, Shaojing, Abdel-Latif, Ahmed, Berron, Brad J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648752/
https://www.ncbi.nlm.nih.gov/pubmed/36355857
http://dx.doi.org/10.1371/journal.pone.0277561
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author Davis, Kara A.
Gottipatti, Anuhya
Peng, Hsuan
Donahue, Renee
Chelvarajan, Lakshman
Cahall, Calvin
Tripathi, Himi
Al-Darraji, Ahmed
Ye, Shaojing
Abdel-Latif, Ahmed
Berron, Brad J.
author_facet Davis, Kara A.
Gottipatti, Anuhya
Peng, Hsuan
Donahue, Renee
Chelvarajan, Lakshman
Cahall, Calvin
Tripathi, Himi
Al-Darraji, Ahmed
Ye, Shaojing
Abdel-Latif, Ahmed
Berron, Brad J.
author_sort Davis, Kara A.
collection PubMed
description Acute myocardial infarction (AMI) results in weakening of the heart muscle and an increased risk for chronic heart failure. Therapeutic stem cells have been shown to reduce inflammatory signaling and scar tissue expansion, despite most of these studies being limited by poor retention of cells. Gelatin methacrylate (GelMA) coatings have been shown to increase the retention of these therapeutic cells near the infarct. In this work, we evaluate two different potential binding partners for GelMA-coated bone marrow cells (BMCs) and myocardial tissue: the extracellular matrix (ECM) and interstitial non-cardiomyocytes. While cells containing β1 integrins mediate cell-ECM adhesion in vivo, these cells do not promote binding to our collagen-degraded, GelMA coating. Specifically, microscopic imagining shows that even with high integrin expression, GelMA-coated BMCs do not bind to cells within the myocardium. Alternatively, BMC incubation with decellularized heart tissue results in higher adhesion of coated cells versus uncoated cells supporting our GelMA-ECM binding mode. To further evaluate the ECM binding mode, cells were incubated on slides modified with one of three different major heart ECM components: collagen, laminin, or fibronectin. While all three components promoted higher adhesion than unmodified glass, collagen-coated slides resulted in a significantly higher adhesion of GelMA-coated BMCs over laminin and fibronectin. Incubation with unmodified BMCs confirmed that without a GelMA coating minimal adhesion of BMCs occurred. We conclude that GelMA cellular coatings significantly increase the binding of cells to collagen within the ECM. Our results provide progress towards a biocompatible and easily translatable method to enhance the retention of transplanted cells in human studies.
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spelling pubmed-96487522022-11-15 Gelatin coating enhances therapeutic cell adhesion to the infarcted myocardium via ECM binding Davis, Kara A. Gottipatti, Anuhya Peng, Hsuan Donahue, Renee Chelvarajan, Lakshman Cahall, Calvin Tripathi, Himi Al-Darraji, Ahmed Ye, Shaojing Abdel-Latif, Ahmed Berron, Brad J. PLoS One Research Article Acute myocardial infarction (AMI) results in weakening of the heart muscle and an increased risk for chronic heart failure. Therapeutic stem cells have been shown to reduce inflammatory signaling and scar tissue expansion, despite most of these studies being limited by poor retention of cells. Gelatin methacrylate (GelMA) coatings have been shown to increase the retention of these therapeutic cells near the infarct. In this work, we evaluate two different potential binding partners for GelMA-coated bone marrow cells (BMCs) and myocardial tissue: the extracellular matrix (ECM) and interstitial non-cardiomyocytes. While cells containing β1 integrins mediate cell-ECM adhesion in vivo, these cells do not promote binding to our collagen-degraded, GelMA coating. Specifically, microscopic imagining shows that even with high integrin expression, GelMA-coated BMCs do not bind to cells within the myocardium. Alternatively, BMC incubation with decellularized heart tissue results in higher adhesion of coated cells versus uncoated cells supporting our GelMA-ECM binding mode. To further evaluate the ECM binding mode, cells were incubated on slides modified with one of three different major heart ECM components: collagen, laminin, or fibronectin. While all three components promoted higher adhesion than unmodified glass, collagen-coated slides resulted in a significantly higher adhesion of GelMA-coated BMCs over laminin and fibronectin. Incubation with unmodified BMCs confirmed that without a GelMA coating minimal adhesion of BMCs occurred. We conclude that GelMA cellular coatings significantly increase the binding of cells to collagen within the ECM. Our results provide progress towards a biocompatible and easily translatable method to enhance the retention of transplanted cells in human studies. Public Library of Science 2022-11-10 /pmc/articles/PMC9648752/ /pubmed/36355857 http://dx.doi.org/10.1371/journal.pone.0277561 Text en © 2022 Davis et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Davis, Kara A.
Gottipatti, Anuhya
Peng, Hsuan
Donahue, Renee
Chelvarajan, Lakshman
Cahall, Calvin
Tripathi, Himi
Al-Darraji, Ahmed
Ye, Shaojing
Abdel-Latif, Ahmed
Berron, Brad J.
Gelatin coating enhances therapeutic cell adhesion to the infarcted myocardium via ECM binding
title Gelatin coating enhances therapeutic cell adhesion to the infarcted myocardium via ECM binding
title_full Gelatin coating enhances therapeutic cell adhesion to the infarcted myocardium via ECM binding
title_fullStr Gelatin coating enhances therapeutic cell adhesion to the infarcted myocardium via ECM binding
title_full_unstemmed Gelatin coating enhances therapeutic cell adhesion to the infarcted myocardium via ECM binding
title_short Gelatin coating enhances therapeutic cell adhesion to the infarcted myocardium via ECM binding
title_sort gelatin coating enhances therapeutic cell adhesion to the infarcted myocardium via ecm binding
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648752/
https://www.ncbi.nlm.nih.gov/pubmed/36355857
http://dx.doi.org/10.1371/journal.pone.0277561
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