Cargando…

Metabolites produced by inoculated Vigna radiata during bacterial assisted phytoremediation of Pb, Ni and Cr polluted soil

Phytoremediation assisted with plant growth promoting bacteria (PGPB) is a green technology to remediate metal contaminated soils. Plants usually produce secondary metabolites to tolerate metal toxicity. Present study was designed to explore the phytoremediation potential of Vigna radiata var. NM-II...

Descripción completa

Detalles Bibliográficos
Autores principales: Zulfiqar, Uzma, Yasmin, Azra, Fariq, Anila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648758/
https://www.ncbi.nlm.nih.gov/pubmed/36355807
http://dx.doi.org/10.1371/journal.pone.0277101
_version_ 1784827646987730944
author Zulfiqar, Uzma
Yasmin, Azra
Fariq, Anila
author_facet Zulfiqar, Uzma
Yasmin, Azra
Fariq, Anila
author_sort Zulfiqar, Uzma
collection PubMed
description Phytoremediation assisted with plant growth promoting bacteria (PGPB) is a green technology to remediate metal contaminated soils. Plants usually produce secondary metabolites to tolerate metal toxicity. Present study was designed to explore the phytoremediation potential of Vigna radiata var. NM-II in the presence of metal resistant PGPB and comparison of metabolites produced under heavy metal stresses (Pb, Ni, Cr). Three PGPB selected for present study include Bacillus pumilus MB246, Serratia nematodiphila MB307 and Delftia Lacustris MB322. Pot experiments were conducted with inoculated V. radiata NM-II seeds grown in soil artificially contaminated with lead (Pb), Nickle (Ni) and chromium (Cr) at a concentration of 300, 200 and 100 mg/kg respectively. After harvesting various growth parameters were studied (root length, shoot length, fresh weight and dry weight). Bacterial colonization on root surfaces of harvested plants was observed through Scanning electron microscopy (SEM) and Elemental composition was recorded through Energy dispersive X-ray spectroscopy (EDX) attached with SEM. Metabolic response of harvested plants was studied through Gas chromatography Mass spectrophotometry (GC-MS) analysis. Metal accumulation in roots, shoots and soil was analysed by acid digestion method from which Bioaccumulation factor (BF) and Translocation factor (TF) of metal from soil to plant was calculated. Results revealed stimulatory effect of PGPB on growth and phytoextraction ability of V. radiata. Soil metal removal efficiency was in the order Pb>Ni>Cr, whereas metal distribution in each part of plant was root>stem>leaf. The BF and TF values suggested V. radiata as Pb and Ni excluder while moderate accumulator for Cr. Elemental analysis through Energy Dispersive X- ray spectroscopy (EDX) found potassium (K(+))and calcium (Ca(+))as highly abundant nutrients with least accumulation of sulphur (S). Metabolites study through GC-MS revealed variety of compounds (carbohydrates, amino acids, fatty acids, steroids etc) detected differentially under each metal treatment and their concentration was influenced by different bacterial inoculations. Overall 9-Octadecenamide was found as commonly present lipid compound in most of the treatments which is required for detoxification in plants. The study concluded beneficial role of PGPB for successful phytoremediation of heavy metals and differential response of metabolites towards each metal stress that is related to metal tolerance ability of V. radiata.
format Online
Article
Text
id pubmed-9648758
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-96487582022-11-15 Metabolites produced by inoculated Vigna radiata during bacterial assisted phytoremediation of Pb, Ni and Cr polluted soil Zulfiqar, Uzma Yasmin, Azra Fariq, Anila PLoS One Research Article Phytoremediation assisted with plant growth promoting bacteria (PGPB) is a green technology to remediate metal contaminated soils. Plants usually produce secondary metabolites to tolerate metal toxicity. Present study was designed to explore the phytoremediation potential of Vigna radiata var. NM-II in the presence of metal resistant PGPB and comparison of metabolites produced under heavy metal stresses (Pb, Ni, Cr). Three PGPB selected for present study include Bacillus pumilus MB246, Serratia nematodiphila MB307 and Delftia Lacustris MB322. Pot experiments were conducted with inoculated V. radiata NM-II seeds grown in soil artificially contaminated with lead (Pb), Nickle (Ni) and chromium (Cr) at a concentration of 300, 200 and 100 mg/kg respectively. After harvesting various growth parameters were studied (root length, shoot length, fresh weight and dry weight). Bacterial colonization on root surfaces of harvested plants was observed through Scanning electron microscopy (SEM) and Elemental composition was recorded through Energy dispersive X-ray spectroscopy (EDX) attached with SEM. Metabolic response of harvested plants was studied through Gas chromatography Mass spectrophotometry (GC-MS) analysis. Metal accumulation in roots, shoots and soil was analysed by acid digestion method from which Bioaccumulation factor (BF) and Translocation factor (TF) of metal from soil to plant was calculated. Results revealed stimulatory effect of PGPB on growth and phytoextraction ability of V. radiata. Soil metal removal efficiency was in the order Pb>Ni>Cr, whereas metal distribution in each part of plant was root>stem>leaf. The BF and TF values suggested V. radiata as Pb and Ni excluder while moderate accumulator for Cr. Elemental analysis through Energy Dispersive X- ray spectroscopy (EDX) found potassium (K(+))and calcium (Ca(+))as highly abundant nutrients with least accumulation of sulphur (S). Metabolites study through GC-MS revealed variety of compounds (carbohydrates, amino acids, fatty acids, steroids etc) detected differentially under each metal treatment and their concentration was influenced by different bacterial inoculations. Overall 9-Octadecenamide was found as commonly present lipid compound in most of the treatments which is required for detoxification in plants. The study concluded beneficial role of PGPB for successful phytoremediation of heavy metals and differential response of metabolites towards each metal stress that is related to metal tolerance ability of V. radiata. Public Library of Science 2022-11-10 /pmc/articles/PMC9648758/ /pubmed/36355807 http://dx.doi.org/10.1371/journal.pone.0277101 Text en © 2022 Zulfiqar et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zulfiqar, Uzma
Yasmin, Azra
Fariq, Anila
Metabolites produced by inoculated Vigna radiata during bacterial assisted phytoremediation of Pb, Ni and Cr polluted soil
title Metabolites produced by inoculated Vigna radiata during bacterial assisted phytoremediation of Pb, Ni and Cr polluted soil
title_full Metabolites produced by inoculated Vigna radiata during bacterial assisted phytoremediation of Pb, Ni and Cr polluted soil
title_fullStr Metabolites produced by inoculated Vigna radiata during bacterial assisted phytoremediation of Pb, Ni and Cr polluted soil
title_full_unstemmed Metabolites produced by inoculated Vigna radiata during bacterial assisted phytoremediation of Pb, Ni and Cr polluted soil
title_short Metabolites produced by inoculated Vigna radiata during bacterial assisted phytoremediation of Pb, Ni and Cr polluted soil
title_sort metabolites produced by inoculated vigna radiata during bacterial assisted phytoremediation of pb, ni and cr polluted soil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648758/
https://www.ncbi.nlm.nih.gov/pubmed/36355807
http://dx.doi.org/10.1371/journal.pone.0277101
work_keys_str_mv AT zulfiqaruzma metabolitesproducedbyinoculatedvignaradiataduringbacterialassistedphytoremediationofpbniandcrpollutedsoil
AT yasminazra metabolitesproducedbyinoculatedvignaradiataduringbacterialassistedphytoremediationofpbniandcrpollutedsoil
AT fariqanila metabolitesproducedbyinoculatedvignaradiataduringbacterialassistedphytoremediationofpbniandcrpollutedsoil